Establishing method of monkey testosterone deficiency model

A technology for establishing a method and a model is applied in the field of establishing a monkey testosterone deficiency model, which can solve the problems of side effects and complications, long-term and frequent medication, inconvenient use, etc., and achieve the effect of reducing toxic and side effects.

Inactive Publication Date: 2018-09-14
SUN YAT SEN UNIV
View PDF0 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are obvious defects in exogenous testosterone drug supplement therapy: (1) Due to individual differences in the level of testosterone decline in patients, it is difficult to control the dose of testosterone supplementation, and it is difficult to be effective if the dose is too small, and side effects and complications are prone to occur if the dose is too large (2) will cause the testosterone level in the body to lose the original circadian rhythm changes, causing multiple complications; (3) inconvenient to use, need long-term frequent medication; (4) use of exogenous testosterone drugs will inhibit the growth of testis Sperm function, long-term use can cause infertility such as oligospermia or azoospermia
However, in primate testes, there are many connective tissue septa emanating from the testicular mediastinum, which divide the testicular parenchyma into many cone-shaped testicular lobules. It is difficult to ensure the uniform distribution and modeling effect of EDS by local injection.
In addition, EDS has obvious hepatotoxicity, and it is also very important to choose the appropriate dosage and administration method for modeling and evaluate the drug damage after modeling, but there are no related reports at home and abroad so far

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Establishing method of monkey testosterone deficiency model
  • Establishing method of monkey testosterone deficiency model
  • Establishing method of monkey testosterone deficiency model

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1: Injecting EDS into the testicular artery combined with blocking the blood flow of the spermatic cord, clearing the Leydig cells of the testis, and establishing a model of testosterone deficiency.

[0020] The animals used in this experiment were adult male cynomolgus monkeys, aged >6 years old, weighing 8-10 kg, a total of 8 adult male cynomolgus monkeys were randomly divided into 4 groups, 2 in each group.

[0021] In the first group (also called "vehicle group"), two cynomolgus monkeys were intra-arterially injected with vehicle (DMSO:H2O, 1:3, V / V) in both testes. EDS was dissolved in vehicle, and 2 cynomolgus monkeys in the second group were injected with 50 mg of EDS into the testicular artery; 2 cynomolgus monkeys in the third group were injected with 100 mg of EDS into the testicular artery; EDS.

[0022] On the day of the experiment, after cynomolgus monkeys were anesthetized, the lower abdomen and perineum were disinfected, and the bilateral spermat...

Embodiment 2

[0027] Example 2: Comparison of the effects of local injection of EDS into the testis of cynomolgus monkeys and injection of EDS into the testicular artery combined with blocking the blood flow of the spermatic cord.

[0028] In addition, 4 adult male cynomolgus monkeys, aged >6 years old and weighing 8-10 kg were selected and randomly divided into 2 groups with 2 monkeys in each group.

[0029] On the day of the experiment, after the cynomolgus monkeys were anesthetized, the lower abdomen and perineum were disinfected, and 2 cynomolgus monkeys in the first group (also called "local group") were locally injected with 100 mg / testis EDS in bilateral testes; Combined group") Two cynomolgus monkeys were exposed to both sides of the spermatic cord by an incision in the inner groin ring, and the blood flow of the spermatic cord was blocked for 20-30 minutes with non-injured forceps; The injection time is 1-5 minutes.

[0030] 0, 1, 2, 3, 4, 5, 7, 9, 11, 13, 15, 20, 25, 30, 35, 40, ...

Embodiment 3

[0032] Example 3: Comparison of the effects of injecting EDS into the testicular artery of cynomolgus monkeys and injecting EDS into the testicular artery combined with blocking the blood flow of the spermatic cord.

[0033] In addition, 4 adult male cynomolgus monkeys were selected, aged >6 years old, weighing 8-10kg, and the 4 adult male cynomolgus monkeys were randomly divided into 2 groups, 2 in each group.

[0034] On the day of the experiment, after the cynomolgus monkeys were anesthetized, the lower abdomen and perineum were disinfected, and 2 cynomolgus monkeys in the first group were injected with 100mg / testis EDS in the bilateral testicular artery; The incision was made to expose the bilateral spermatic cords, and the blood flow of the spermatic cords was blocked for 20-30 minutes with non-invasive forceps; the testis was exposed by an incision in the scrotum, and an equal amount of EDS was slowly injected into the testicular artery for 1-5 minutes.

[0035] 0, 1, 2,...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides an establishing method of a monkey testosterone deficiency model, which adopts a way of arterially injecting dimethyl sulfonate ethane to testicle of an adult monkey and blocking the spermatic cord blood flow. By adopting the method, a safe and stable testosterone deficiency animal model can be provided.

Description

technical field [0001] The invention belongs to the field of animal models, and in particular relates to a method for establishing a monkey testosterone deficiency model. Background technique [0002] Testosterone deficiency syndrome (TDS) refers to the lack of testosterone levels in the body due to various reasons in different periods of a man's life, resulting in abnormal morphology and function of his target organs, which in turn causes corresponding clinical symptoms. It is a male reproductive endocrine disease that seriously affects male health, life expectancy and quality of life. TDS mainly includes late-onset hypogonadism (late-onset hypogonadism (LOH)), congenital hypogonadism, testicular damage and other diseases. With the aging process of Chinese society, the incidence of TDS is increasing year by year, and the medical market demand is very huge. It has become a major disease that seriously affects the physical and mental health of our people. At present, the cl...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/255
CPCA61K31/255A61K31/10A01K2207/20A01K2227/106A01K2267/0306A01K67/027
Inventor 项鹏柯琼夏凯邓春华毛富祥
Owner SUN YAT SEN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap