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A kind of supramolecular hydrogel of chitosan and gelatin and its preparation method and application

A supramolecular hydrogel, chitosan technology, applied in the field of biological materials, can solve the problems of low critical dissolution temperature of gelatin, non-reversible sol and gel transition and injectability, etc., and achieve good biocompatibility , the effect of reducing the wound

Active Publication Date: 2019-09-24
HUAZHONG UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The present invention aims at the problem that chitosan and gelatin composite hydrogel contains toxic chemical cross-linking agent, does not have reversible sol and gel transition and injectability, and the critical solution temperature on gelatin is too low, and provides a solution Supramolecular hydrogel of chitosan and gelatin, preparation method and application thereof

Method used

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  • A kind of supramolecular hydrogel of chitosan and gelatin and its preparation method and application
  • A kind of supramolecular hydrogel of chitosan and gelatin and its preparation method and application
  • A kind of supramolecular hydrogel of chitosan and gelatin and its preparation method and application

Examples

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Embodiment 1

[0030] A preparation method of a supramolecular hydrogel of chitosan and gelatin, comprising the following steps:

[0031](1) Synthesis of chitosan modified by carboxylated derivatives of β-cyclodextrin: Weigh 4.0 g of carboxymethyl β-cyclodextrin and dissolve it in 80 mL of deionized water, add 3.6 g of 1-(3-dimethylaminopropyl base)-3-ethylcarbodiimide hydrochloride and 2.2g N-hydroxysuccinimide mixture, fully stirred and mixed for 2h to activate the carboxyl group of carboxymethyl β-cyclodextrin to obtain activated Carboxymethyl β-cyclodextrin solution: Weigh 4g chitosan and dissolve it in 400mL 1% acetic acid solution, add to the activated carboxymethyl β-cyclodextrin solution, mix well, and stir at room temperature for 24h. Dialyze with a dialysis bag with a molecular weight cut-off of 2,000 for 3 days, then centrifuge at 10,000 rpm for 5 minutes, collect the supernatant, and freeze-dry to obtain carboxymethyl β-cyclodextrin-modified chitosan;

[0032] (2) Preparation of...

Embodiment 2

[0034] Based on Example 1, the difference is: the amount of chitosan modified by carboxymethyl β-cyclodextrin in step (2) is changed to 10 mg, and all the others are the same as in Example 1.

Embodiment 3

[0036] Based on Example 1, the difference is: the amount of chitosan modified by carboxymethyl β-cyclodextrin in step (2) is changed to 20 mg, and all the other are the same as in Example 1.

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Abstract

The invention provides supermolecular hydrogel containing chitosan and gelatin as well as a preparation method and an application of the supermolecular hydrogel and belongs to the field of biomaterials. The supermolecular hydrogel contains a part formed by combining chitosan modified with a beta-cyclodextrin carboxylated derivative and gelatin under the host-guest interaction; chitosan is graftedwith the beta-cyclodextrin carboxylated derivative through an amide group. The preparation method comprises the steps as follows: the chitosan is grafted with the activated beta-cyclodextrin carboxylated derivative through the amide group, then, the gelatin is added, so that the grafted beta-cyclodextrin carboxylated derivative and an aromatic group of the gelatin are in host-guest interaction, and the supermolecular hydrogel containing the chitosan and the gelatin is obtained. The supermolecular hydrogel containing the chitosan and the gelatin has shear thinning and self-healing characteristics and can be used as an injectable cell or drug carrier. The beta-cyclodextrin group in hydrogel and the aromatic group in the gelatin can be used in cell carriers and tissue engineering materials through the host-guest interaction.

Description

technical field [0001] The invention belongs to the field of biological materials, and in particular relates to a supramolecular hydrogel of chitosan and gelatin, a preparation method and application thereof. Background technique [0002] Chitosan is a kind of polysaccharide with abundant resources, which has good biocompatibility and degradability, and is an excellent material for preparing hydrogels. At present, the preparation method of chitosan hydrogel is mainly the chemical cross-linking method, through the reaction of chemical cross-linking agent with chitosan amino, hydroxyl and other active groups to form a hydrogel network; but chemical cross-linking agents are often cytotoxic, The application of chitosan hydrogel in cell carrier and tissue engineering is limited. At the same time, the chemically cross-linked chitosan hydrogel does not have thixotropy and reversibility, and cannot enter the body by injection. [0003] Gelatin is a decomposition product of collage...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08J3/075C08L89/00C08L5/08C08B37/08
Inventor 杨光肖林周凤黄丽霞刘莉
Owner HUAZHONG UNIV OF SCI & TECH
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