Preparation of soybean molasses biological cellulose moisture mask
A technology of biological cellulose and soybean molasses, applied in the directions of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve problems such as no reports, and achieve the effects of reducing cost, prolonging action time, and being difficult to evapotranspiration.
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Embodiment 1
[0043] A kind of preparation of embodiment 1 soybean molasses biological cellulose moisturizing facial mask, its steps are:
[0044] (1) Preparation of soybean molasses biocellulose base film
[0045] 1) Preparation of medium
[0046] Seed medium: 2% glucose, 0.5% yeast extract powder, 0.5% peptone, 0.27% disodium hydrogen phosphate, 0.115% citric acid, make up the rest with water, adjust pH=6;
[0047] Molasses medium: soybean molasses solid content 15Brix, corn gluten powder 0.15%, zinc sulfate 0.15%, malic acid 0.4%, adjust PH=6;
[0048] The culture medium was sterilized by high pressure steam, irradiated with ultraviolet light and cooled to room temperature;
[0049] 2) Inoculation static culture
[0050] Komagataeibacter intermedius CGMCC12562 (Komagataeibacter intermedius CGMCC12562) were inoculated in the seed medium for expansion, cultured in a shaker incubator (30°C, 160r) for 22h, and then inoculated in molasses medium, when the bacterial cellulose membrane Take i...
Embodiment 2
[0068] The preparation of embodiment 2 a kind of soybean molasses biological cellulose moisturizing mask, its steps are:
[0069] (1) Preparation of soybean molasses biocellulose base film
[0070] 1) Preparation of medium
[0071] Seed medium: 2% glucose, 0.5% yeast extract powder, 0.5% peptone, 0.27% disodium hydrogen phosphate, 0.115% citric acid, make up the rest with water, adjust pH=6;
[0072] Molasses medium: soybean molasses solid content 15Brix, corn gluten powder 0.15%, zinc sulfate 0.15%, malic acid 0.4%, adjust PH=6;
[0073] The culture medium was sterilized by high pressure steam, irradiated with ultraviolet light and cooled to room temperature;
[0074] 2) Inoculation static culture
[0075] Komagataeibacter intermedius CGMCC12562 (Komagataeibacter intermedius CGMCC12562) were inoculated in the seed medium for expansion, cultured in a shaker incubator (30°C, 160r) for 22h, and then inoculated in molasses medium, when the bacterial cellulose membrane Take it...
Embodiment 3
[0093] Embodiment 3 A kind of preparation of soybean molasses biological cellulose moisturizing mask, its steps are:
[0094] (1) Preparation of soybean molasses biocellulose base film
[0095] 1) Preparation of medium
[0096] Seed medium: 2% glucose, 0.5% yeast extract powder, 0.5% peptone, 0.27% disodium hydrogen phosphate, 0.115% citric acid, make up the rest with water, adjust pH=6;
[0097] Molasses medium: soybean molasses solid content 15Brix, corn gluten powder 0.15%, zinc sulfate 0.15%, malic acid 0.4%, adjust PH=6;
[0098] The culture medium was sterilized by high pressure steam, irradiated with ultraviolet light and cooled to room temperature;
[0099] 2) Inoculation static culture
[0100] Komagataeibacter intermedius CGMCC12562 (Komagataeibacter intermedius CGMCC12562) were inoculated in the seed medium for expansion, cultured in a shaker incubator (30°C, 160r) for 22h, and then inoculated in molasses medium, when the bacterial cellulose membrane Take it out...
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