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Pseudomonas aeruginosa, method for screening same and application of pseudomonas aeruginosa to lactic acid fermentation on straw

A Pseudomonas aeruginosa, screening method technology, applied in the directions of fermentation, microorganism-based methods, biochemical equipment and methods, etc., can solve the problems of loss, damage to cell membrane, activity reduction, etc., to improve straw conversion rate, reduce environmental Pollution, the effect of ensuring food security

Active Publication Date: 2018-08-10
HUAZHONG AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the conversion rate of lactic acid to straw in this invention is not high, and crop straw needs to be pretreated before it can be used by microorganisms. During the pretreatment process, straw will produce a variety of by-products, which inhibit the metabolism of microorganisms. The main sources are lignin and Decomposition of hemicellulose, including small molecule organic acids, furans and phenols, etc.
Most microorganisms cannot utilize lignin, and lignin and some of its degradation products will adsorb cellulase during the fermentation process, forming co-precipitation, reducing its activity to loss; lignin decomposition produces ferulic acid, coniferous aldehyde and other phenols These compounds can improve the fluidity of microbial cell membranes, lead to a large loss of potassium ions, damage the cell membranes, and inhibit the metabolism of microorganisms, thus affecting the conversion rate of lactic acid to straw

Method used

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  • Pseudomonas aeruginosa, method for screening same and application of pseudomonas aeruginosa to lactic acid fermentation on straw
  • Pseudomonas aeruginosa, method for screening same and application of pseudomonas aeruginosa to lactic acid fermentation on straw
  • Pseudomonas aeruginosa, method for screening same and application of pseudomonas aeruginosa to lactic acid fermentation on straw

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1 Screening of strain FL02

[0026] 1. Add 90ml enrichment medium (1g / L vanillin+1g / L p-hydroxybenzaldehyde+1g / L syringaldehyde+1g / L yeast extract powder, pH7.0) into a 250ml Erlenmeyer flask, and then add them separately The sludge in the second settling tank of the water treatment plant and the soil near the outlet of the printing and dyeing plant were each 10g, sealed and placed on a shaker at 37°C, shaking at 150r / min for 24h to obtain an enriched culture solution.

[0027] 2. Fill a 250ml Erlenmeyer flask with 100ml screening medium (1g / L vanillin+1g / L p-hydroxybenzaldehyde+1g / L syringaldehyde+1g / L guaiacol+1g / L ferulic acid+1g / L coumaric acid+0.5g / L yeast extract powder+0.2g / L MgSO 4 7H 2 O,0.1g / L CaCl 2 ,1g / L(NH 4 ) 2 SO 4 ,1g / L K 2 HPO 4 ,0.1g / L NaCl,0.02g / L FeCl 3 , PH 7.0), respectively add 1ml of enriched culture solution, seal it and place it on a shaker at 37°C, shake culture at 150r / min for 48h to obtain a screening seed solution.

[0028] 3. After gra...

Embodiment 2

[0029] Example 2. Identification of strain FL02

[0030] The 16S rRNA sequence of strain FL02 is shown in SEQ ID NO:1, and the 16S rRNA gene of the strain was amplified by PCR using primers 27F (5'-AGAGTTTGATCCTGGCTCAG-3') and 1492R (5'-TAC GGCTACCTTGTTACGACTT-3'). Sequence analysis and homology comparison of 16S rRNA sequence in GenBank showed that the similarity between strain FL02 and Pseudomonas aeruginosa was 99%. Strain FL02, after morphological identification, physiological and biochemical experiments, 16S rRNA sequence analysis and homology comparison, the similarity between strain FL02 and Pseudomonas aeruginosa was 99%, so the strain Pseudomonas aeruginosa was determined to be Pseudomonas aeruginosa.

[0031] The microbiological characteristics of strain FL02 are as follows: the bacteria are slender and vary in length, sometimes in the shape of a ball or thread, arranged in pairs or short chains. One end of the bacterial body has a single flagella. Observed under a dark...

Embodiment 3

[0035] Example 3. Degradation effect of strain FL02 on coumaric acid, p-hydroxybenzaldehyde, vanillin, ferulic acid and syringaldehyde

[0036] The degradation or utilization status of Pseudomonas aeruginosa FL02 screened by the present invention on coumaric acid, p-hydroxybenzaldehyde, vanillin, ferulic acid and syringaldehyde is detected.

[0037] The specific test process is as follows: Take 5 equal parts of the Pseudomonas aeruginosa strain (Pseudomonas aeruginosa) FL02 screened by the present invention and add them to the medium 1-5, each of which contains 0.5g / L Yeast extract powder, 0.2g / L MgSO 4 .7H 2 O,0.1g / L CaCl 2 ,1g / L(NH 4 ) 2 SO 4 ,1g / L K 2 HPO 4 ,0.1g / L NaCl,0.02g / L FeCl 3 , And the medium 1-5 contains 1g / L vanillin, 1g / L p-hydroxybenzaldehyde, 1g / L syringaldehyde, 1g / L coumaric acid, 1g / L ferulic acid, and Pseudomonas aeruginosa FL02 can use the above compounds as the sole carbon source, cultured at 150r / min and 37°C for 36 hours. The results show that Pseudomonas a...

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Abstract

The invention provides pseudomonas aeruginosa, a method for screening the same and application of the pseudomonas aeruginosa to lactic acid fermentation on straw. A preservation number of the pseudomonas aeruginosa is CCTCC NO:M2018169. The pseudomonas aeruginosa, the method and the application have the advantages that the pseudomonas aeruginosa is acquired from activated sludge in aerobic ponds of municipal sewage treatment plants by means of screening, diversified typical products generated in lignin pretreatment procedures can be efficiently degraded by the pseudomonas aeruginosa, and the degradation rates of the pseudomonas aeruginosa for various substrates including cumaric acid, parahydroxybenzaldehyde, vanillin, ferulic acid and syringaldehyde can reach 100% after culture is carriedout under the condition of the temperatures of 37 DEG C for 36 hours; the typical products can be prevented from inhibiting microbial metabolism during the lactic acid fermentation on the straw whenthe pseudomonas aeruginosa is used for carrying out the lactic acid fermentation on the straw, accordingly, the straw conversion rate of lactic acid can be increased, the pseudomonas aeruginosa is short in fermentation period, and the fermentation is accomplished after culture is carried out for 48-72 h; the plant straw is used as a fermentation raw material, accordingly, waste can be turned intowealth, environmental pollution can be reduced, and the pseudomonas aeruginosa, the method and the application have positive significance in guaranteeing the food safety.

Description

Technical field [0001] The invention relates to the field of microbiology and straw lactic acid fermentation, in particular to Pseudomonas aeruginosa and its screening method, and the application of Pseudomonas aeruginosa in straw lactic acid fermentation. Background technique [0002] China is a large agricultural country, and the annual output of crop straw exceeds 800 million tons. Crop stalks are mainly used for returning to the field, silage and yellow storage, biogas, biochar, biomass fuel and bioenergy, etc., but the direct return of straws to the field is slow to decompose and does not significantly improve the quality of cultivated land; as silage or yellow storage , The production cycle is long, and the feed conversion rate is not high; the fermentation of straw to produce biogas will produce a large amount of biogas slurry and biogas residue, and the large amount of straw incineration causes serious haze. All in all, crop straw resources are used in a large amount, bu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/02C12P7/56C12R1/385
CPCC12N1/02C12P7/56C12N1/205C12R2001/385
Inventor 胡金龙彭楠李红蔡玉缘谢月娇周文兵梁运祥朱端卫
Owner HUAZHONG AGRI UNIV
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