Detection method of traditional Chinese medicine ingredients in Chenxianglubailu tablets
A detection method, the technology of Bailu Tablets, is applied in the direction of measuring devices, material separation, and analysis of materials. The effect of promoting intestinal peristalsis
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[0028] Experimental example: content determination of liquiritin, rutin naringin and hesperidin
[0029] (1) Selection of detection wavelength
[0030] Referring to the relevant literature and using high-performance liquid chromatography, an appropriate amount of mixed reference substance was taken for injection, and a diode array detector was used to scan in the wavelength range of 190-800nm. The results showed that the maximum absorption wavelength of liquiritin was 277nm, and the maximum absorption wavelength of rutin naringin was 283nm, the maximum absorption wavelength of hesperidin is 284nm, choose a wavelength that can detect the above three active ingredients at the same time, and finally determine the detection method for the simultaneous determination of liquiritin, rutin naringin and hesperidin in Chenxianglu Bailu Tablets The wavelength is 277nm.
[0031] (2) Selection of mobile phase
[0032] Mobile phase 1: methanol-0.5% acetic acid (32:68);
[0033] Mobile ph...
Embodiment
[0096] A detection method of traditional Chinese medicine components in Chenxianglu Bailu Tablets, comprising:
[0097](1) Chromatographic conditions and system suitability experiment: the chromatographic column is Phenomenex Gemini C18 (250mm×4.6mm, 5μm); with acetonitrile-0.2% acetic acid (19:81) as the mobile phase; the detection wavelength is 277nm; the column temperature is 30 ℃; the flow rate is 1ml / min; the number of theoretical plates should not be less than 5000 based on the peak of liquiritin.
[0098] (2) Preparation of reference solution: Accurately weigh the appropriate amount of reference substances of liquiritin, rutin naringin, and hesperidin, add methanol to make a solution containing 20 μg of liquiritin, 20 μg of rutin and 100 μg of hesperidin per 1 ml , that is;
[0099] (3) Preparation of the test solution: Grind Chenxianglu Bailu Tablets finely, take about 0.5g, accurately weigh it, put it in a stoppered conical flask, add 50ml of methanol precisely, weig...
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