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Biological fluorescent probe for detecting nickel ions and preparation method thereof

A fluorescent probe and nickel ion technology, applied in the field of fluorescence detection, can solve the problems of insufficient response speed, weak selectivity, complex synthesis, etc., and achieve the effects of convenient operation, extensive industrial value, and simple synthesis

Inactive Publication Date: 2018-07-27
GUANGXI TEACHERS EDUCATION UNIV
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0004] However, the currently reported fluorescent probes for detection still have some problems, such as poor selectivity, insufficient response speed, complex synthesis, poor water solubility, etc.

Method used

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  • Biological fluorescent probe for detecting nickel ions and preparation method thereof
  • Biological fluorescent probe for detecting nickel ions and preparation method thereof
  • Biological fluorescent probe for detecting nickel ions and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0042] The invention provides a biological fluorescent probe for detecting nickel ions and a preparation method thereof.

[0043] The preparation method of the bioluminescent probe for detecting nickel ions comprises the following steps:

[0044] S1: Mix 2,6-dichloro-3-cyano-4picoline and hydrazine hydrate until dissolved to obtain a mixture;

[0045] S2: After condensing and refluxing the mixture, the crystals were precipitated by cooling, filtered, washed, and dried to obtain a light yellow intermediate product A;

[0046] S3: Weighing 1-phenyl-1,3-butanedione, dissolving it in an ethanol solution and dropping it into the intermediate product A, cooling to room temperature after the reaction, filtering, washing, and drying to obtain a bioluminescence probe L;

[0047] The reaction process is as follows:

[0048]

[0049] see figure 1 , is the bioluminescent probe (10μM) to different metals (50μM, Li + , Na + , K + , Mg 2+ , Ca 2+ , Ba 2+ , Mn 2+ , Fe 2+ ,Co 2+...

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Abstract

The invention provides a biological fluorescent probe for detecting nickel ions and a preparation method thereof. The biological fluorescent probe is a Ni2+ fluorescent probe with rapid and high sensitivity, the probe has high anti-interference capability for other common metal ions, and the probe is used for qualitative and quantitative determination of nickel ions in environment and cells. The preparation method is simple, operation is convenient, result stability is good, and the method is easy to carry out and promote. The probe and the method have good application values for nickel ion detection. Convenience of detection process is greatly improved. The demands in practical application processes are accorded with.

Description

technical field [0001] The invention relates to the technical field of fluorescence detection, in particular to a bioluminescent probe for detecting nickel ions and a preparation method thereof. Background technique [0002] Metal ions widely present in nature play important roles in environmental, medical, biological, and chemical sciences. Although some of these heavy metals and transition metal ions are low in the organism, most of them exist in the form of metalloproteins and metalloenzymes, and participate in many important reactions, information transmission, and energy transfer in the organism. It has an important influence on the development of metabolism and also occupies an important position in medicinal chemistry. Nickel ions are essential elements for the human body and certain crops. Although the toxicity of nickel and its salts is low, due to its biochemical activity, it can activate or inhibit a series of enzymes (arginase, carboxylase, acid phosphatase and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D471/14C09K11/06G01N21/64
Inventor 顾运琼胡飞龙
Owner GUANGXI TEACHERS EDUCATION UNIV
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