Method for preparing gamma-aminobutyric acid by feeding substrate enzyme

A technology of aminobutyric acid and substrates, which is applied in the field of feeding substrate enzymes to prepare γ-aminobutyric acid, can solve the problems of low catalytic reaction stability, unfavorable industrial production, and prone to substrate inhibition, etc. Inhibition of substances, effective control, and good reproducibility

Inactive Publication Date: 2018-07-20
NANTONG LICHENG BIOLOGICAL ENG
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  • Description
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  • Application Information

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Problems solved by technology

[0005] It can be seen that most of the methods in the prior art are to add substrates to the buffer system containing bacteria, which has the disadvantages of low catalytic reaction stability, poor enzyme activity stability, easy substrate inhibition, and easy pollution, which is not conducive to large-scale production. chemical industrial production

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  • Method for preparing gamma-aminobutyric acid by feeding substrate enzyme

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Embodiment 1

[0032] The invention discloses a method for preparing gamma-aminobutyric acid (GABA) by feeding substrate enzymes, respectively using citric acid-sodium citrate, acetic acid-sodium acetate and disodium hydrogen phosphate of 0.05mol / L and pH=4.5 -Three kinds of buffer solutions of citric acid dissolve the substrate sodium glutamate, prepare a mass fraction of 4% sodium glutamate solution; then adopt a membrane filter to filter the sodium glutamate solution to remove impurities and remove bacteria; After the enrichment culture, the bacterial slurry was obtained by membrane filtration; the bacterial slurry was divided into three parts, and transferred to three enzyme-catalyzed reaction tanks for stirring. The reaction temperature was controlled at 35°C, the pH was 4.5, and the stirring speed was adjusted to 100rpm, add three kinds of sodium glutamate solutions after impurity removal to the three reaction tanks continuously, control the concentration of residual sodium glutamate so...

Embodiment 2

[0036] The invention discloses a method for preparing gamma-aminobutyric acid (GABA) by feeding substrate enzymes. The substrate sodium glutamate is dissolved in a disodium hydrogen phosphate-citric acid buffer solution of 0.05 mol / L and pH=4.5. Prepare sodium glutamate solutions with mass fractions of 2%, 4% and 6% respectively; then use a membrane filter to filter the sodium glutamate solution to remove impurities and eliminate bacteria; enrich and cultivate microorganisms, and pass through the membrane Filter to obtain the bacterial slurry; divide the bacterial slurry into 3 parts, and transfer to three enzyme-catalyzed reaction tanks for stirring respectively. Three kinds of sodium glutamate solutions with different mass fractions after impurity removal are added continuously in the internal flow, and the concentration of residual sodium glutamate solution is controlled between 10-45g / L. After each reaction for 36 hours, the bacterial cells are removed by membrane filtratio...

Embodiment 3

[0039] The invention discloses a method for preparing gamma-aminobutyric acid (GABA) by feeding substrate enzymes. The substrate sodium glutamate is dissolved in a disodium hydrogen phosphate-citric acid buffer solution of 0.05 mol / L and pH=4.5. Prepare a sodium glutamate solution with a mass fraction of 4%; then use a membrane filter to filter the sodium glutamate solution to remove impurities and remove bacteria; enrich and cultivate the microorganisms, and obtain a bacterium slurry by membrane filtration after the end; Divide the slurry into 4 parts and transfer them to four enzyme-catalyzed reaction tanks for stirring respectively. Continuously add 4% sodium glutamate solution after removal of impurities in the tank, control the concentration of residual sodium glutamate solution between 10-45g / L, after each reaction for 36 hours, remove bacteria by membrane filtration, and obtain enzymolysis clear liquid , then detect the content of GABA prepared in four reaction tanks by...

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Abstract

The invention discloses a method for preparing gamma-aminobutyric acid by feeding a substrate enzyme. A substrate monosodium glutamate is added into a buffer solution to prepare a monosodium glutamatesolution, the monosodium glutamate solution is filtered, and the filtered monosodium glutamate solution is fed to a bacterial slurry, so the fluctuation of the substrate is reduced, and the stable proceeding of an enzymatic reaction is facilitated; and the substrate dissolved buffer solution is used to continuously dilute a reaction system, so the enzyme content in per unit volume stably changes,and the enzyme activity is continuously stable. The method for preparing gamma-aminobutyric acid by feeding the substrate enzyme has the advantages of good buffering effect in the buffer system, andhigh yield.

Description

technical field [0001] The invention belongs to the field of biocatalysis, and in particular relates to a method for preparing gamma-aminobutyric acid by feeding substrate enzymes. Background technique [0002] Gamma-aminobutyric acid (GABA), also known as aminobutyric acid, is an amino acid widely distributed in nature as the main inhibitory transmission substance of nerves. For example, it exists in tomatoes, mandarin oranges, grapes, potatoes, eggplants, pumpkins and other vegetables and fruits, and many fermented or germinated foods and grains also contain GABA, such as pickles, pickles, germinated rice, etc. It has physiological effects such as stress relief, sleep improvement, brain strengthening, blood pressure lowering, beauty treatment, and weight loss. [0003] The production methods of GABA include chemical synthesis, enzymatic method, fermentation method and so on. The production of GABA by chemical synthesis, which accounts for the main output, does not comply...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P13/00
CPCC12P13/005
Inventor 金锋顾钦青李江波孙怡夏洪志尹忠燕
Owner NANTONG LICHENG BIOLOGICAL ENG
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