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A biomimetic composite scaffold material doped with graphene and its construction method

A technology of composite scaffold and construction method, applied in tissue regeneration, prosthesis, medical science, etc.

Inactive Publication Date: 2020-10-20
DALIAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no relevant report on the composite of chitosan, β-sodium glycerophosphate, gelatin, and graphene for cartilage tissue engineering biomimetic repair scaffolds.

Method used

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  • A biomimetic composite scaffold material doped with graphene and its construction method
  • A biomimetic composite scaffold material doped with graphene and its construction method
  • A biomimetic composite scaffold material doped with graphene and its construction method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1 Preparation of Cs / GP / Gel biomimetic cartilage composite scaffold material (Cs / GP / Gel mass ratio 1:15.2:3.7) (as a comparative example)

[0030] Weigh 2.2 g of chitosan powder, add it into 100 mL of 0.1 mol / L acetic acid solution, and stir it magnetically for 2 h at 40° C. to make it evenly mixed. Centrifuge at 1000rpm for 0.5h to degas and deslag the chitosan acetic acid solution. Weigh 20.0 g of sodium β-glycerophosphate powder, add it into 20 mL of deionized water, and stir magnetically at room temperature for 30 min to dissolve it evenly. Weigh 6.0 g of gelatin particles, add to 100 mL of deionized water, and stir magnetically for 30 min at 40°C to make them evenly mixed. At room temperature, the prepared chitosan acetic acid solution was stirred rapidly on a magnetic stirrer. During the stirring process, the β-sodium glycerophosphate solution was slowly added drop by drop, and the solution was fully stirred for 30 minutes to prepare a Cs / GP solution. Obs...

Embodiment 2

[0031] Example 2 Preparation of Cs / GP / Gel biomimetic cartilage composite scaffold material (Cs / GP / Gel mass ratio 1:6.7:1.6) (as a comparative example)

[0032] Weigh 2.5 g of chitosan powder, add it into 100 mL of 0.1 mol / L acetic acid solution, and stir magnetically for 3 h at 50° C. to make it evenly mixed. Centrifuge at 1000rpm for 0.5h to degas and deslag the chitosan acetic acid solution. Weigh 24.0 g of sodium β-glycerophosphate powder, add it to 16 mL of deionized water, and stir magnetically at room temperature for 40 min to dissolve it evenly. Weigh 7.0 g of gelatin particles, add to 100 mL of deionized water, and stir magnetically for 40 min at 50°C to mix evenly. At room temperature, the prepared chitosan acetic acid solution was stirred rapidly on a magnetic stirrer. During the stirring process, the β-sodium glycerophosphate solution was slowly added drop by drop, and the solution was fully stirred for 30 minutes to prepare a Cs / GP solution. Observe that there is...

Embodiment 3

[0033] Example 3 Preparation of Cs / GP / Gel biomimetic cartilage composite scaffold material (Cs / GP / Gel mass ratio 1:6.7:1.0) (as a comparative example)

[0034] Weigh 3.0 g of chitosan powder, add it into 100 mL of 0.1 mol / L acetic acid solution, and stir it magnetically for 4 hours at 50° C. to make it evenly mixed. Centrifuge at 1000rpm for 1h to degas and deslag the chitosan acetic acid solution. Weigh 30.0 g of sodium β-glycerophosphate powder, add it to 10 mL of deionized water, and stir magnetically at room temperature for 60 min to dissolve it evenly. Weigh 8.0 g of gelatin particles, add to 100 mL of deionized water, and mix evenly with a magnetic stirrer at 60°C for 60 min. At room temperature, the prepared chitosan acetic acid solution was stirred rapidly on a magnetic stirrer. During the stirring process, the β-sodium glycerophosphate solution was slowly added drop by drop, and the solution was fully stirred for 60 minutes to prepare a Cs / GP solution. Observe that ...

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Abstract

The invention belongs to the field of tissue engineering scaffold materials, and provides a bionic composite scaffold material doped with graphene and an establishment method of the bionic composite support material. The establishment method comprises the following steps: firstly preparing a Cs acetic acid solution, a GP aqueous solution, a Gel aqueous solution and a graphene suspension, and thenmixing the Cs acetic acid solution and the GP aqueous solution to prepare a Cs / GP solution A; adding the Gel aqueous solution into the Cs / GP solution A to prepare a Cs / GP / Gel solution B; and adding the graphene suspension into the Cs / GP / Gel solution B to prepare a Cs / GP / Gel / Gr solution C, transferring the solution C into a low-temperature mold, freeze drying at a low temperature, and performing the subsequent treatment, thus obtaining the bionic cartilage composite support material. A mass ratio of chitosan Cs to beta-sodium glycerophosphate GP to gelatin Gel to graphene Gr in the bionic cartilage composite scaffold material is 1: (5 to 20): (1 to 5): (0.02 to 0.08). The bionic composite scaffold material has the advantages of simple process, easy control and low production cost; and a produced support product is uniform in porosity, has an inter-connective pore structure and has an excellent bionic effect.

Description

technical field [0001] The invention belongs to the field of tissue engineering scaffold materials, in particular to a method for constructing tissue engineering bionic cartilage composite scaffold materials by chitosan, sodium β-glycerophosphate, gelatin and graphene. Background technique [0002] The osteochondral tissue structure of normal joints is mainly composed of three parts: hyaline cartilage, calcified cartilage layer and subchondral bone. Due to the huge amount of articular cartilage used in various activities, it is very easy to cause damage in trauma and acute and chronic inflammation, mostly cartilage wear, tear, fracture and comminuted damage. Joint prosthesis replacement is currently one of the effective treatment methods, but this method is not only expensive, but also has the risk of complications. In addition, the articular cartilage is extremely fragile and has low self-repair ability. The cartilage itself has no blood supply, and its nutrition mainly co...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/44A61L27/46A61L27/48A61L27/52A61L27/58A61L27/50
CPCA61L27/443A61L27/46A61L27/48A61L27/50A61L27/52A61L27/58A61L2430/06C08L5/08C08L89/00
Inventor 宋克东卢延国李丽颖李文芳胡雪岩刘天庆
Owner DALIAN UNIV OF TECH
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