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Ginsenoside-insulin nano gel as well as preparation method and application thereof

A technology of ginsenoside and nanogel, which is applied in the direction of medical formula, medical preparations with non-active ingredients, and medical preparations containing active ingredients. release, the effect of small particle size

Pending Publication Date: 2018-07-13
FUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to reduce the side effects caused by existing insulin injections, solve the problems of physical and psychological pain and high price of insulin injections to patients, the present invention provides a ginsenoside-insulin nanogel and its preparation method and application, It has the advantages of long duration of drug effect and economic convenience

Method used

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  • Ginsenoside-insulin nano gel as well as preparation method and application thereof
  • Ginsenoside-insulin nano gel as well as preparation method and application thereof
  • Ginsenoside-insulin nano gel as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] 1) Weigh 5g of total ginsenosides, add acetic acid solution with pH 1.8 to 25ml, decompose at 90°C for 4h, leave the reactant at room temperature for 12h, filter with a 0.45μm filter membrane to remove insoluble matter, and use 10% sodium carbonate for the filtrate Neutralize to a pH of 8, then filter with a 2 μm filter membrane, and collect the precipitate; add the precipitate to 20 mL of absolute ethanol, heat to 60°C to dissolve it, leave it to cool at 4°C for 2 hours, and then filter it with a 2 μm filter paper. The filtrate was concentrated under reduced pressure and dried to obtain ginsenoside gel powder;

[0036] 2) Take insulin and ginsenoside gel powder, add secondary water, so that the final concentration of ginsenoside in the solution is 1~40 mg / mL, and the concentration of insulin is 1~25U / mL, and ultrasonically shake for 15 minutes to obtain ginsenoside / Insulin nanogel nanoparticle solution;

[0037] 3) Dissolve 0.5g of carbomer, 0.35g of gelatin, 0.5mg o...

Embodiment 2

[0038] Embodiment 2 Characterization of ginsenoside-insulin nanogel:

[0039] 1) Use dynamic light scattering (DLS) to measure the particle size (size, nm) and potential (zeta potential, mv) of the nanogel, the results are as follows figure 1 shown.

[0040] Depend on figure 1 It can be seen that the nanogel prepared by the present invention has a small particle size and a negative potential.

[0041] 2) The three-dimensional structure of the nanogel was determined by atomic force microscopy (AFM), and the results are as follows figure 2 shown.

[0042] Depend on figure 2 It can be seen that the ginsenoside / insulin prepared by the present invention is in the form of gel.

Embodiment 3

[0043]Example 3 In vitro percutaneous absorption test:

[0044] 1) In vitro percutaneous absorption experiment in mice

[0045] At room temperature, prepare an appropriate amount of BCA working solution by adding 50 volumes of BCA reagent A and 1 volume of BCA reagent B (50:1), and mix well. BCA working solution is stable at room temperature for 24 hours. Completely dissolve the protein standard, take 10 μL and dilute to 100 μL to make the final concentration 0.5mg / ml, and dilute the diluted standard (0.5mg / ml) at 0, 2, 4, 6, 8, 12, 16 , 20 μL were added to EP tubes, and PBS was added to make up to 20 μL. Add 200 μL of BCA working solution to each tube, place it at 37°C for 30 minutes, and draw the standard curve of protein quantification according to the absorbance value at the wavelength between 562nm and the results are as follows: image 3 shown.

[0046] Depend on image 3 It can be seen that the linearity of the protein content determined by the BCA method is good. ...

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Abstract

The invention discloses ginsenoside-insulin nano gel as well as a preparation method and application thereof. A hypoglycemic drug insulin used as a drug effect ingredient is combined with ginsenosidegel nano-particles having an effect of reducing blood sugar, so that the ginsenoside-insulin nano gel having double blood sugar reduction effects is prepared. By using the ginsenoside gel nano-particles, slow-release transdermal drug administration of insulin can be realized, and a side effect caused by injecting the insulin is avoided. The ginsenoside-insulin nano gel disclosed by the invention is used for preparing a transdermal drug administration preparation and has the characteristics of quick transdermal absorption rate, security, stability, no pain, convenience and the like; moreover, the preparation method has the advantages of simpleness, low production cost, and easiness for amplification.

Description

technical field [0001] The invention belongs to the technical field of pharmaceutical preparations, and in particular relates to a ginsenoside-insulin nanogel and its preparation method and application. Background technique [0002] Diabetes is a metabolic disease characterized by high blood sugar, and has become the "third killer" that endangers human health after cardiovascular and cerebrovascular diseases and tumors. According to the report of the International Diabetes Federation (IDF), there were 425 million people with diabetes worldwide in 2017, among which the number of people with diabetes in China was as high as 114.4 million, ranking first in the world, and it is expected to increase to 150.7 million by 2040. For diabetic patients, once the blood sugar is not well controlled for a long time, it is likely to lead to serious complications, such as diabetic retinopathy will lead to blindness, diabetic nephropathy will lead to chronic renal failure and other complicat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/28A61K36/258A61K9/06A61K47/42A61K47/32A61P3/10
CPCA61K9/0014A61K9/06A61K36/258A61K38/28A61K47/32A61K47/42A61K2300/00
Inventor 邵敬伟郭燕李超
Owner FUZHOU UNIV
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