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Identification method of mussaenda pubescens

A technology of Jade Leaf Golden Flower and identification method, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., and can solve the problems of low accuracy of identification results

Inactive Publication Date: 2018-07-10
SINOPHARM GRP DEZHONG (FOSHAN) PHARM CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In the prior art, the identification method of Yuye Jinhua is that an experienced Chinese pharmacist compares it with the above-mentioned traits after manual observation and smell, and the accuracy of the identification result is low.

Method used

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  • Identification method of mussaenda pubescens
  • Identification method of mussaenda pubescens
  • Identification method of mussaenda pubescens

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] The preparation of need testing solution: get need testing powder 4g, add ethanol 20mL, heat and reflux 30min, filter while hot, take filtrate and obtain need testing solution;

[0060] Preparation of reference substance solution: Take 4 g of Yuye Jinhua reference substance powder, add 20 mL of ethanol, heat to reflux for 30 minutes, filter while hot, and take the filtrate to obtain the reference substance solution;

[0061] Thin-layer chromatography plate: ordinary silica gel G prefabricated plate.

[0062] Spotting: Spot 4 μL of the test solution and the reference solution respectively.

[0063] Developing solvent: cyclohexane-acetone-ethyl acetate (volume ratio 4:2:1).

[0064] Expansion method: upward expansion, the expansion distance is 8cm.

[0065] Color development: spray 10% sulfuric acid ethanol solution, and bake at 105°C until the spots are clear.

[0066] Inspection: View under ultraviolet light (365nm).

[0067] Chromatogram identification: In the chro...

Embodiment 2

[0071] Preparation of the test solution: take 2 g of the test powder, add 20 mL of ethanol, heat to reflux for 20 min, filter while hot, and take the filtrate to obtain the test solution;

[0072] Preparation of reference substance solution: Take 2 g of Yuye Jinhua reference substance powder, add 20 mL of ethanol, heat to reflux for 20 minutes, filter while hot, and take the filtrate to obtain the reference substance solution;

[0073] Thin-layer chromatography plate: ordinary silica gel G prefabricated plate.

[0074] Spotting: Spot 4 μL of the test solution and the reference solution respectively.

[0075] Developing solvent: cyclohexane-acetone-ethyl acetate (volume ratio 4:2:1).

[0076] Expansion method: upward expansion, the expansion distance is 8cm.

[0077] Color development: spray 10% sulfuric acid ethanol solution, and bake at 105°C until the spots are clear.

[0078] Inspection: View under ultraviolet light (365nm).

[0079] Chromatogram identification: In the ...

Embodiment 3

[0081] Preparation of the test solution: take 3 g of the test powder, add 20 mL of ethanol, heat to reflux for 25 min, filter while hot, and take the filtrate to obtain the test solution;

[0082] Preparation of reference substance solution: Take 3 g of Yuye Jinhua reference substance powder, add 20 mL of ethanol, heat to reflux for 25 minutes, filter while hot, and take the filtrate to obtain the reference substance solution;

[0083] Thin-layer chromatography plate: ordinary silica gel G prefabricated plate.

[0084] Spotting: Spot 4 μL of the test solution and the reference solution respectively.

[0085] Developing solvent: cyclohexane-acetone-ethyl acetate (volume ratio 4:2:1).

[0086] Expansion method: upward expansion, the expansion distance is 8cm.

[0087] Color development: spray 10% sulfuric acid ethanol solution, and bake at 105°C until the spots are clear.

[0088] Inspection: View under ultraviolet light (365nm).

[0089] Chromatogram identification: In the ...

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Abstract

The invention discloses an identification method of mussaenda pubescens. The identification method of the mussaenda pubescens comprises the following steps: sucking 2-12mu L of test sample solution and 2-12mu L of mussaenda pubescens reference solution, separately dripping the test sample solution and the reference solution onto the same thin layer chromatography plate, spreading the test sample solution and the reference solution on the thin layer chromatography plate by using a developing solvent, taking out the thin layer chromatography plate, and drying in the air; spraying the thin layerchromatography plate with an ethanol sulfate solution containing 10% developer, heating until the spots on the thin layer chromatography plate are chromogenic and clear, and viewing the thin layer chromatography plate under the sunlight or lamp light; when the spots of the same color in the chromatogram of a test sample is displayed at the position corresponding to the chromatogram of the reference substance of the mussaenda pubescens, determining that the test sample is the mussaenda pubescens. The treatment method of the test sample is completely the same as that of the mussaenda pubescens reference substance, and the test sample and the mussaenda pubescens reference substance are spread for developing in the same thin layer chromatography plate, so that whether the test sample is the mussaenda pubescens or not can be precisely determined according to the comparison result; therefore, the identification method, provided by the invention, of the mussaenda pubescens is high in accuracyrate of identification results.

Description

technical field [0001] The invention relates to the technical field of identification of traditional Chinese medicines, in particular to an identification method of Jade Leaf Golden Flower. Background technique [0002] Jade leaf golden flower is a commonly used Chinese herbal medicine. The medicinal part is its tender leaves. It has the functions of clearing heat and detoxifying, promoting dampness and reducing swelling. It can be used for the treatment of colds, heatstroke, enteritis, nephritis edema, sore throat and bronchitis. Dried young leaves of Yuye Jinhua are often shrunken, curled and broken, and the appearance is taupe or light black. The complete leaves are ovate-oblong or ovate-lanceolate after being flattened, with a length of 5-8cm and a width of 2-2.5cm , apex acuminate, base cuneate, top glabrous or sparsely hairy, bottom densely pubescent, veins more obvious on the back; crisp, slightly fragrant, slightly bitter taste. [0003] In the prior art, the identi...

Claims

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Application Information

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IPC IPC(8): G01N30/90
CPCG01N30/90
Inventor 杜蔼媚
Owner SINOPHARM GRP DEZHONG (FOSHAN) PHARM CO LTD
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