Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of realgar microbiological leaching liquid in preparation of drug for inhibiting angiogenesis

A technology of microbial leaching and angiogenesis, applied in the field of medicine, can solve the problems of unsatisfactory effect, transformation, and high toxicity of nano-realgar, and achieve the effects of reducing angiogenesis, inhibiting proliferation, and inhibiting angiogenesis

Inactive Publication Date: 2018-07-03
LANZHOU UNIVERSITY
View PDF9 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Mineral drug realgar has anti-tumor effect, because realgar is difficult to dissolve in water, so in order to solve the solubility problem of realgar, the patent "Nano-realgar and its preparation method" with the patent authorization announcement number of CN1116028C involves a kind of nano-realgar, the nano-realgar At present, it is a realgar product that inhibits tumor angiogenesis better than other realgar products. However, because nano-realgar only changes in physical properties, it cannot dissolve or transform the effective chemical components in realgar. Therefore, nano-realgar inhibits tumor angiogenesis. The effect of blood vessels is still not ideal, and the toxicity is relatively high

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of realgar microbiological leaching liquid in preparation of drug for inhibiting angiogenesis
  • Application of realgar microbiological leaching liquid in preparation of drug for inhibiting angiogenesis
  • Application of realgar microbiological leaching liquid in preparation of drug for inhibiting angiogenesis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] The preparation of embodiment 1 realgar microbial leachate

[0042] Get Acidithiobacillus ferrooxidans (preserved in China Center for Type Culture Collection on July 23, 2004, preservation number: CCTCC-M 204057, preservation name: Acidithiobacillus ferrooxidans BY3-1), use 200 μg sub Sodium arsenate / 100ml 9K medium, 400μg sodium arsenite / 100ml 9K medium, 600μg sodium arsenite / 100ml 9K medium, 800μg sodium arsenite / 100ml 9K medium, 1600μg sodium arsenite / 100ml 9K medium Medium gradient acclimation, and then take 0.1g realgar / 100ml 9K medium, 0.2g realgar / 100ml 9K medium, 0.3g realgar / 100ml 9K medium, 0.5g realgar / 100ml 9K medium, 1.0g realgar / 100ml 9K culture Then gradually reduce the amount of ferrous sulfate in the medium. When the amount of ferrous sulfate is 3K, the domesticated Thiobacillus ferrooxidans can be obtained.

[0043] Take 1g / L Fe 2+ Add 0.5 g of realgar powder to 90 ml of OK culture medium of ferrous sulfate, inoculate 10 ml of domesticated Thiobacill...

Embodiment 2

[0045] Example 2 Comparing the effects of realgar microbial leachate and nano-realgar with different arsenic concentrations on the viability of HUVEC cells

[0046] Purpose and principle: MTT method was used to detect the proliferation of HUVEC. MTT (3-(4,5-dimethylthiazole-2)-2,5-diphenyltetrazolium bromide) is a yellow dye that accepts hydrogen ions and can act on respiration in the mitochondria of living cells chain, under the action of succinate dehydrogenase and cytochrome C, the tetrazole ring cracks to generate blue formazan crystals (Formazan), and the amount of formazan crystals is only proportional to the number of living cells (succinic acid in dead cells Dehydrogenase disappears, MTT cannot be reduced). Formazan crystals generated by reduction can be dissolved in the MTT solution containing 10% (m / v) sodium lauryl sulfate, 5% (m / v) isobutanol and 0.012mol / L hydrochloric acid, and can be determined by microplate reader The optical density OD value at 570nm can ind...

Embodiment 3

[0050] Example 3 Realgar Microbial Leaching Liquid Inhibits HUVEC Cell Migration Experiment

[0051] Purpose and principle: The cell scratch test is an easy-to-operate and cheap in vitro experimental method for studying cell migration, by artificially creating a blank area (called scratch) on the fused monolayer cells. Gradual migration of cells at the edge of the scratch into the clear area "heals" the scratch.

[0052] Method: HUVEC cells were seeded in a 6-well plate, and after 80% of the cells reached confluence. Use a pipette tip to make a light scratch in the middle of each hole. Add the cell culture solution containing 0, 0.5, 1.0 and 2.0 μg / ml realgar microbial leaching solution, place in CO 2 After 12 h of culture in the cell culture incubator, the fresh medium was replaced. Take pictures under a microscope to observe and count the migration of HUVECs. The cell migration rate was calculated according to the following formula:

[0053] Cell migration rate (%) = nu...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Apertureaaaaaaaaaa
Login to View More

Abstract

The invention mainly relates to application of realgar microbiological leaching liquid in the preparation of a drug for inhibiting angiogenesis. The realgar microbiological leaching liquid is obtainedby biologically converting realgar in acclimatized ferrous oxide thiobacillus and is capable of effectively inhibiting the proliferation, migration, invasion and microtubule formation of vascular endothelial cells, reducing the angiogenesis in chick chorioallantoic membranes and remarkably inhibiting the angiogenesis of tumor bodies of H22 solid tumor mice so as to inhibit the growth of tumors; and the realgar microbiological leaching liquid can be used for treating new vessel tolerance diseases and related diseases such as tumors, arthritis, psoriasis, ophthalmic diseases and atherosclerosis.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to the application of realgar microbiological leachate in the preparation of drugs for inhibiting angiogenesis. Background technique [0002] Angiogenesis refers to the process in which new blood vessels form mature blood vessels at all levels through remodeling and expansion. New vessels often sprout out from existing mature tissue, or split into smaller daughter vessels from a large vasculature. Angiogenesis plays an important role in the body's normal physiological processes such as embryonic development, reproduction, wound repair, and the female menstrual cycle [1] . Angiogenesis is a complex process involving a variety of molecules from a variety of cells. At present, the dynamic balance between angiogenesis-inhibiting molecules and angiogenesis-promoting molecules is considered to be a "switch" to regulate angiogenesis. Under normal physiological conditions, the two are ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K33/36A61P35/00A61P35/02A61P17/06A61P9/10A61P27/02A61P19/02A61P29/00
CPCA61K33/36
Inventor 李红玉宋鹏李洋蒋云龙
Owner LANZHOU UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com