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High-density culture method of saccharomyces cerevisiae and pH regulation and control method of saccharomyces cerevisiae

A technology for high-density culture and Saccharomyces cerevisiae, which is applied in the field of high-density culture of Saccharomyces cerevisiae and pH regulation in the fermentation process, can solve problems such as hysteresis, potential safety hazards, long cycle, etc. sufficient effect

Active Publication Date: 2018-06-29
ANGELYEAST CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0015] The technical problem solved by the present invention is: the high-density culture period in the prior art is long, and the off-line parameter alcohol concentration is used as the signal for feedback regulation and feeding feeding, so there is a hysteresis defect, the utilization rate of raw materials is low, and the cell density of the product is low; In the prior art, it is necessary to use acid and alkali to adjust the pH, which has potential safety hazards

Method used

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  • High-density culture method of saccharomyces cerevisiae and pH regulation and control method of saccharomyces cerevisiae
  • High-density culture method of saccharomyces cerevisiae and pH regulation and control method of saccharomyces cerevisiae
  • High-density culture method of saccharomyces cerevisiae and pH regulation and control method of saccharomyces cerevisiae

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Effect test

Embodiment 1

[0082] This embodiment is a method for regulating the high-density cultivation of Saccharomyces cerevisiae based on online physiological parameters, and the steps include:

[0083] a. Treatment of fermented raw materials: remove slag and precipitate molasses, add concentrated sulfuric acid to acidify to pH 2.0, and hydrolyze molasses to obtain fermented molasses with reducing sugar weight accounting for 20%; yeast extract powder is added with water to make a solution with a concentration of 20%; The molasses and yeast infusion solution was sterilized at 110°C for 10 minutes.

[0084] b. Seed culture: first-level seed culture, insert a ring of bacteria (Saccharomyces cerevisiae FX-2) from the strain storage slope into 200ml first-level medium, and cultivate at 30°C and 220rpm for 18 hours; second-level seed culture According to 10% of the volume of the secondary medium, the seeds of the primary culture were inserted into 1L of the secondary medium, and cultivated at 28°C and 20...

Embodiment 2

[0091] This embodiment is a high-density culture method for regulating Saccharomyces cerevisiae based on online physiological parameters, and the steps include:

[0092] a. Fermentation raw material treatment: molasses removes slag and precipitates, adds concentrated hydrochloric acid to acidify to pH2.5, hydrolyzes molasses to obtain fermented molasses with reducing sugar weight accounting for 25%, yeast extract powder is added with water to form a solution with a concentration of 25%; The molasses and yeast infusion solution was sterilized at 110°C for 10 minutes.

[0093] b. Seed culture: first-level seed culture, insert a ring of bacteria (Saccharomyces cerevisiae FX-2) from the strain storage slope into 200ml first-level medium, and cultivate at 28°C and 200rpm for 20h; second-level seed culture Inoculate 1L of the secondary medium according to the inoculation amount of 12% of the volume of the secondary medium, and culture at 30°C and 220rpm for 20h;

[0094] By weight,...

Embodiment 3

[0100] This embodiment is a high-density culture method for regulating Saccharomyces cerevisiae based on online physiological parameters, and the steps include:

[0101] a. Fermentation raw material treatment: molasses deslagging and precipitation, adding concentrated nitric acid to acidify to pH 3.5, so that molasses is hydrolyzed to obtain fermented molasses with reducing sugar weight accounting for 22%, yeast extract powder is added with water to form a solution with a concentration of 22%; The molasses and yeast infusion solution was sterilized at 110°C for 10 minutes.

[0102] b. Seed culture: first-level seed culture, insert a ring of bacteria (Saccharomyces cerevisiae FX-2) from the strain storage slope into 250ml first-level medium, and cultivate at 32°C for 26h at 300rpm; second-level seed culture , according to the inoculum amount of 15% of the volume of the secondary medium, 1L of the secondary medium was inserted, and cultured at 32° C. and 300 rpm for 25 hours.

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Abstract

The invention provides a high-density culture method of saccharomyces cerevisiae and a pH regulation and control method of the saccharomyces cerevisiae. The high-density culture method comprises the following steps: performing first-stage and second-stage seed enlarging cultivation; centrifugating, washing and enriching seeds; putting the seeds into a fermentation tank filled with water for fermental cultivation, and feeding molasses and a yeast extract powder solution during fermentation. The pH regulation and control method comprises the following steps: monitoring a physiological state of thallus in the fermentation tank on line and in real time, and regulating and controlling the speed of feeding molasses and yeast extract powder according to a carbon dioxide release speed, an oxygen intake speed and changes of a respiratory quotient, so as to regulate and control the pH value in the fermentation tank, wherein no inorganic salt, acid, alkali and trace elements are required to be added in the whole fermentation, regulation and control process; at the end of fermentation, performing separation and washing, and cooling saccharomyces cerevisiae thallus. The method disclosed by theinvention is easy to operate and low in cost; the prepared saccharomyces cerevisiae is edible and has high protein content; a fermentation raw material is high in conversion rate; no acid and alkali are added, so that the operation safety is improved.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a high-density cultivation method of brewer's yeast and a pH regulation method in the fermentation process. Background technique [0002] Yeast is a pure natural, pollution-free, biological health source of nutrition, with more than 50 kinds of nutrients, including complete protein, vitamin B complex, nucleic acid, etc. necessary for the human body, and is praised as the most attractive nutrition by the nutritional circles Taste. The water content in yeast cells is 65-70%, of which about 15% is free water; 35-45% of carbohydrates, mainly in the form of polysaccharides; 35-45% of proteins, including monomeric proteins and binding Protein; 3-5% fat, mainly lecithin and sterol; 5-10% ash, mainly potassium, phosphorus content. In addition, it also contains a variety of vitamins, with the most B vitamins. Saccharomyces cerevisiae has the characteristics of high reproduction...

Claims

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Application Information

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IPC IPC(8): C12N1/18C12Q3/00C12R1/865
CPCC12N1/18C12Q3/00
Inventor 李啸俞学锋李知洪谈亚丽伍业旭
Owner ANGELYEAST CO LTD
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