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Bacillus amyloliquefaciens with effects of inorganic phosphorus degradation and disease prevention

A technology for dissolving starch spores and inorganic phosphorus is applied in the field of agricultural microorganisms, which can solve the problems of single function of Bacillus, and achieve the effects of being difficult to drug resistance, reducing the amount of phosphorus fertilizer application, and reducing production costs.

Active Publication Date: 2018-06-22
齐永新
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The functions of the above-mentioned known bacillus are relatively single, or they only have the function of dissolving phosphorus, or only have the function of preventing and controlling diseases; secondly, with the change of environmental conditions or the evolution of pathogenic bacteria, the function of bacillus may also evolve and change accordingly Therefore, separating and screening Bacillus with high-efficiency phosphorus-dissolving function and wide antibacterial spectrum from soil can adjust the contradiction between supply and demand of soil phosphorus, improve the effect of plant disease prevention, reduce production costs, protect the ecological environment, and promote agricultural production. The sustainable development of

Method used

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  • Bacillus amyloliquefaciens with effects of inorganic phosphorus degradation and disease prevention
  • Bacillus amyloliquefaciens with effects of inorganic phosphorus degradation and disease prevention
  • Bacillus amyloliquefaciens with effects of inorganic phosphorus degradation and disease prevention

Examples

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Effect test

Embodiment 1

[0048] Example 1 Isolation and screening process and classification identification of bacterial strain WKPHO-12 of the present invention

[0049] (1) the separation and screening process of bacterial strain of the present invention:

[0050] In September 2015, 5 soil samples were collected at five points in the Fanshan phosphate mine area, Zhulu County, Zhangjiakou City, Hebei Province, each 200g. Weigh 1.0g of air-dried soil samples and add them to the Erlenmeyer flask with sterilized glass beads, then add 99mL of sterile water, let it stand for 20min, fully shake it on the shaker at 30°C and 180r / min for 30min, and then proceed according to the 10-fold dilution method Gradient dilution, respectively take 10 -3 、10 -4 、10 -5 100 μL of the diluted solution of the above solution was applied on the inorganic phosphorus solution medium, and each concentration was repeated three times. 7d. The strains capable of degrading inorganic phosphorus were screened by the transparent ...

Embodiment 2

[0059] Embodiment 2 The present invention contains the preparation of the microbial bacterial agent of WKPHO-12 bacterial strain

[0060] Follow the steps below:

[0061] (1) Strain activation: activate the Bacillus amyloliquefaciens strain WKPHO-12 (its preservation number is CGMCC No.14954) stored at -80°C on LB plate medium (30°C), pick a single colony on Cultivate 12 hours at 30°C on LB slant medium to obtain activated bacterial strains; the composition and weight ratio of LB plate medium or LB slant medium are: tryptone 10g, yeast extract 5g, sodium chloride 5g, Agar powder 15g, water 1000mL;

[0062] (2) Preparation of seed liquid: 100 mL of LB liquid culture medium (its composition and weight ratio: tryptone 10 g, yeast extract 5 g, sodium chloride 5 g, water 1000 mL) was placed in a 250 mL conical flask, and high pressure damp heat Sterilize, after the temperature drops to room temperature, insert an inoculation loop into each bottle of the activated strain in step (...

Embodiment 3

[0066] Example 3 Qualitative determination test of bacterial strain WKPHO-12 of the present invention to degrade inorganic phosphorus

[0067] Proceed as follows:

[0068]With sterilized toothpick, the WKPHO-12 bacterial strain activated in the embodiment 2 step (1) is spot-inoculated on the solution inorganic phosphorus plate medium (its composition and weight ratio are: glucose 10.0g, (NH ) 2 SO 4 0.5g, MgSO 4 ·7H 2 O 0.5g, NaCl 0.2g, Ca 3 (PO 4 )2 5.0g, KCl 0.2g, MnSO 4 0.03g, FeSO 4 0.003g, 20.0g agar, 1000mL distilled water, pH: 7.0-8.0), and then placed in a constant temperature incubator at 30°C for 5 days to measure the diameter of the transparent circle and the diameter of the colony.

[0069] Results The strain WKPHO-12 of the present invention contained Ca 3 (PO 4 ) 2 A transparent circle with a diameter of 13.6 millimeters is produced on the inorganic phosphorus plate medium, indicating that the WKPHO-12 bacterial strain of the present invention can wel...

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Abstract

The invention provides a bacillus amyloliquefaciens strain WKPHO-12. A collection number of the strain is CGMCC No. 14954. The invention further discloses a microbial agent comprising the strain. Thestrain WKPHO-12 has the effects of degradation of inorganic phosphorus in soil and disease prevention, can efficiently degrade the inorganic phosphorus in the soil, increases a utilization ratio of the inorganic phosphorus in the soil, reduces an application amount of a phosphate fertilizer, and has a high prevention effect on plant diseases such as eggplant verticillium wilt; the average prevention effect is 70% or higher; the strain WKPHO-12 has a wide antibacterial spectrum and a good inhibiting effect on eggplant verticillium wilt bacteria, cucumber fusarium wilt bacteria, fusarium wilt bacteria, potato black scurf bacteria and the like; in addition, the strain WKPHO-12 is strong in specialty, long in biocidal effect duration and environmentally friendly, and can greatly lower production cost and promote agricultural sustainable development.

Description

technical field [0001] The invention belongs to the field of agricultural microbes, and in particular relates to a bacillus amyloliquefaciens with dual functions of degrading inorganic phosphorus and preventing diseases, and also relates to microbial agents containing the bacillus amyloliquefaciens, and their effects in preventing and controlling crop diseases and promoting crop growth Applications. Background technique [0002] Phosphorus is not only one of the three major elements in plant nutrition, but also an important component of organic compounds in plants, which plays an indispensable role in the growth and development of plants. Although the phosphorus content in the soil is relatively high, about 95% of it is in an invalid form, and the inorganic phosphorus that can be directly absorbed and utilized by plants only accounts for 2-3% of the total phosphorus. 74% of the cultivated land in my country is deficient in phosphorus. If chemical fertilizers are used for a ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/21A01N63/00A01P3/00C09K17/14C09K101/00C12R1/07
CPCA01N63/00C09K17/14C09K2101/00C12N1/20C12N1/205C12R2001/07
Inventor 马毅楠李博超孔令佳
Owner 齐永新
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