A method for preparing composite oil nanoemulsion by low temperature dissolution of phospholipid-microfluidic technology

A low-temperature dissolving and compound oil technology, which is applied in the field of medicine, can solve the problems of active oxidative substances affecting the physiological function of the liver, increasing the content of aspartate aminotransferase, and abnormal metabolism of liver enzymes, so as to improve the uniformity of particle size distribution and reduce oxidation and hydrolysis. , to avoid the effect of phospholipid oxidation

Active Publication Date: 2021-01-12
NINGBO NINGRONG BIOPHARMA CO LTD
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Problems solved by technology

It has been reported that when the PFAT5 in the emulsion is 0.1%, the content of aspartate aminotransferase in plasma increases significantly after 72 hours of infusion in rats, the metabolism of liver enzymes is abnormal, and the liver has reached a state of pathological damage, suggesting that long-term emulsion infusion Note, lipid droplets in the liver will accumulate, initially producing fatty liver, and then turning into liver inflammation and leading to liver cell necrosis. In addition, the active oxidative substances produced will affect the normal physiological function of the liver

Method used

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  • A method for preparing composite oil nanoemulsion by low temperature dissolution of phospholipid-microfluidic technology
  • A method for preparing composite oil nanoemulsion by low temperature dissolution of phospholipid-microfluidic technology
  • A method for preparing composite oil nanoemulsion by low temperature dissolution of phospholipid-microfluidic technology

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Experimental program
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Effect test

Embodiment 1

[0062] Take 60g of refined soybean oil, 60g of medium-chain triglycerides, 50g of refined olive oil, 30g of purified fish oil and 135mg of dl-α-tocopherol, add them to No. 1 pressure-resistant preparation tank, and take the egg yolk Add 12 grams of phospholipids to it, preheat the cavity of the tank to 30° C., fill the tank with carbon dioxide gas until the pressure in the cavity is 7 MPa, and disperse the phospholipids in the mixed oil to obtain an oil phase. In No. 2 preparation tank, 25 grams of glycerin, 300 mg of sodium oleate and 10 ml of sodium hydroxide solution (2 mg / ml) were dissolved in 763 g of water for injection to prepare an aqueous phase (pH of 9.8 to 11.5), heated to 30°C. Then, the water phase is added to the oil phase, and the stator rotor type shearing machine is used for shear dissolution to prepare colostrum. After that, slowly release the gas in the No. 1 preparation tank to restore the pressure in the tank to normal atmospheric pressure. The original c...

Embodiment 2

[0064] Take 60g of refined soybean oil, 60g of medium-chain triglycerides, 50g of refined olive oil, 30g of purified fish oil and 135mg of dl-α-tocopherol, add them to No. 1 pressure-resistant preparation tank, and take the egg yolk Add 12 grams of phospholipids to it, preheat the cavity of the tank to 30° C., fill the tank with carbon dioxide gas until the pressure in the cavity is 7.5 MPa, and disperse the phospholipids in the mixed oil to obtain an oil phase. In No. 2 preparation tank, 25 grams of glycerin, 300 mg of sodium oleate and 10 ml of sodium hydroxide solution (2 mg / ml) were dissolved in 763 g of water for injection to prepare an aqueous phase (pH of 9.8 to 11.5), heated to 30°C. Then, the water phase is added to the oil phase, and the stator rotor type shearing machine is used for shear dissolution to prepare colostrum. After that, slowly release the gas in the No. 1 preparation tank to restore the pressure in the tank to normal atmospheric pressure. The original...

Embodiment 3

[0066] Take 60g of refined soybean oil, 60g of medium-chain triglycerides, 50g of refined olive oil, 30g of purified fish oil and 135mg of dl-α-tocopherol, add them to No. 1 pressure-resistant preparation tank, and take the egg yolk Add 12 grams of phospholipids to it, preheat the cavity of the tank to 31° C., fill the tank with carbon dioxide gas until the pressure in the cavity is 7.2 MPa, and disperse the phospholipids in the mixed oil to obtain an oil phase. In No. 2 preparation tank, 25 grams of glycerin, 300 mg of sodium oleate and 10 ml of sodium hydroxide solution (2 mg / ml) were dissolved in 763 g of water for injection to prepare an aqueous phase (pH 9.8 to 11.5), heated to 31° C. Then, the water phase is added to the oil phase, and the stator rotor type shearing machine is used for shear dissolution to prepare colostrum. After that, slowly release the gas in the No. 1 preparation tank to restore the pressure in the tank to normal atmospheric pressure. The original c...

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Abstract

The invention relates to a method for preparing a composite grease nanoemulsion by adopting a phospholipid low-temperature dissolution-microjet technology. The method comprises the steps of (1) dissolving phospholipid in grease to prepare an oil phase; (2) mixing and dissolving components of an aqueous phase to prepare an aqueous phase; (3) adding the aqueous phase obtained in step (2) into the oil phase obtained in step (1), and performing shearing to prepare a primary emulsion; (4) using the microjet technology for processing the primary emulsion to prepare the emulsion. By adopting the method, the stability of the prepared emulsion can be improved, the oxidation and hydrolysis of the phospholipid are reduced, the oxidation of polyunsaturated fatty acids is reduced, peroxide and methoxyaniline values are reduced, the uniformity of particle size distribution of the emulsion is improved, and the proportion of PFAT5 is reduced.

Description

technical field [0001] The invention belongs to the technical field of medicine, and relates to a method for preparing compound oil nanoemulsion by low-temperature dissolution of phospholipids and micro-fluidization technology. Background technique [0002] Fat emulsion is one of the important components of total parenteral nutrition (TPN). In addition to providing the energy required for the body's metabolism, it also provides the body with polyunsaturated fatty acids required for the metabolism of biofilms and bioactive substances, and it can be used in conjunction with amino acid and glucose infusion to provide maintenance without oral intake Various nutrients needed by the body under stress. [0003] In recent years, more and more attention has been paid to the research on the inhibition of inflammatory expression and the therapeutic effect of omega-3 unsaturated fatty acids on cardiovascular and cerebrovascular diseases, which has promoted the development of omega-3 un...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/107A61K47/24
CPCA61K9/1075A61K47/24
Inventor 甘勇王晓丽张馨欣柯琴季亮朱春柳
Owner NINGBO NINGRONG BIOPHARMA CO LTD
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