Preparation method of beef cattle feed
A technology for feed and beef cattle, which is applied in the field of beef cattle breeding, can solve the problems of stimulating the weight growth of beef cattle and improving the quality of beef cattle, and the nutrition of beef cattle feed is not comprehensive, and achieves the effects of improving disease resistance, improving nutritional value, and increasing the content of active ingredients.
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preparation example Construction
[0028] A preparation method for beef cattle feed, comprising the steps of:
[0029](1) According to the number of parts by mass, take 290-300 parts of wheat grains, 5-10 parts of Polygonum, 155-200 parts of cassava dregs, 0.5-0.8 parts of calcium carbonate, and 100-120 parts of nutrient salt solution, and adjust the pH to 5.0~5.2, stir evenly, sterilize at 121°C for 20 minutes to obtain a sterilized mixture, take the culture solution of Aspergillus niger, Saccharomyces cerevisiae and Bacillus licheniformis and mix it according to the mass ratio of 5:5:1 to obtain a mixed bacterial solution, and inoculate at 13% Inoculate an appropriate amount into the sterilized mixture, ferment and cultivate at 28-35°C for 4-6 days, take the fermented product and dry it, crush it through a 100-mesh sieve, collect the sieved particles, and set aside;
[0030] (2) Take the pretreated Trichosanthes seeds and mix them with n-hexane at a mass ratio of 1:9, ultrasonicate at 58~60°C and 150~180W for...
Embodiment 1
[0034] Aspergillus niger, Saccharomyces cerevisiae, Bacillus licheniformis: Stored on slopes at -80°C in the laboratory.
[0035] Aspergillus niger culture medium: inoculate Aspergillus niger into PDA liquid medium, culture at 25°C for 5 days, pick the culture medium and add 0.1% Tween-80 at a mass ratio of 1:10 to elute spores to obtain Aspergillus niger culture medium; Yeast culture medium: inoculate Saccharomyces cerevisiae into YPD liquid medium, culture at 25°C, 180rpm for 12 hours to obtain Saccharomyces cerevisiae culture medium; Bacillus licheniformis culture medium: inoculate Bacillus licheniformis into liquid LB medium, 30°C, 180rpm Shake culture for 12 hours to obtain Bacillus licheniformis culture solution.
[0036] Nutrient salt solution: in parts by mass, 50 parts of glucose, 75 parts of urea, 25 parts of potassium dihydrogen phosphate, and 100 parts of water are mixed.
[0037] Pretreatment of Trichosanthes seeds: Powder the dried Trichosanthes seeds and pass t...
Embodiment 2
[0046] Aspergillus niger, Saccharomyces cerevisiae, Bacillus licheniformis: Stored on slopes at -80°C in the laboratory.
[0047] Aspergillus niger culture solution: inoculate Aspergillus niger into PDA liquid medium, culture at 27°C for 6 days, pick the culture solution and add 0.1% Tween-80 at a mass ratio of 1:10 to elute spores to obtain Aspergillus niger culture solution; Yeast culture medium: inoculate Saccharomyces cerevisiae into YPD liquid medium, culture at 30°C, 180rpm for 13 hours to obtain Saccharomyces cerevisiae culture medium; Bacillus licheniformis culture medium: inoculate Bacillus licheniformis into liquid LB medium, 33°C, 180rpm Shake culture for 17 hours to obtain Bacillus licheniformis culture solution.
[0048] Nutrient salt solution: in parts by mass, 55 parts of glucose, 78 parts of urea, 27 parts of potassium dihydrogen phosphate, and 120 parts of water are mixed.
[0049] Pretreatment of Trichosanthes seeds: Powder the dried Trichosanthes seeds and ...
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