Biochemical detection micro-fluidic chip, biochemical detection micro-fluidic chip system and applications thereof
A technology of microfluidic chip and biochemical detection, which is applied in the field of biochemical detection microfluidic chip system, biochemical detection, and biochemical detection microfluidic chip, which can solve the problem of difficulty in ensuring detection accuracy, lack of reagent storage function, Increase the risk of operation and other issues to achieve the effect of strong detection repeatability, avoid detection interference, and avoid cross-infection
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preparation example Construction
[0100] The preparation method of a preferred biochemical detection microfluidic chip provided by the present invention preferably includes the following steps (taking the chip including the liquid storage unit 2 as an example):
[0101] Step 1) prepack the reagents required for the biochemical reaction into the reagent pouch 21, and seal the sealing port 212 of the reagent pouch 21 by means of thermoplastic sealing; and place it in the chip system through the positioning hole 211 At the specific liquid storage structure of the middle structure, the reagent pouches 21 for each liquid storage are further fixed on the middle structure of the chip system by means of thermoplastic sealing.
[0102] Step 2) Different freeze-dried solid powders are placed inside each different reaction unit 3 in the middle layer structure of the chip, and the weight of the solid powder placed in each reaction unit 3 is constant.
[0103] Step 3) Bonding the lower structure to the lower part of the up...
Embodiment 1
[0117] This example is used to illustrate the preparation of the chip and chip system provided by the present invention and the multi-index detection of whole blood using the chip system
[0118] The biochemical indicators in whole blood to be detected in this embodiment are: alanine aminotransferase (ALT), aspartate aminotransferase (AST), γ-glutamyltransferase (γ-GT), alkali Phosphatase (ALP), total protein (TP), albumin (Alb), total cholesterol (TC), triglyceride (TG).
[0119] Various detection reagents and sample diluents to be used in this example were purchased from a Leadman Biochemical Reagent Company in Beijing. The processing method for each detection reagent is to obtain respective solid powders after freeze-drying each liquid reagent.
[0120] (1) Assembly of chip system
[0121] The above 300 μL diluent reagent is prepacked in the reagent pouch 21, and the sealing port 212 of the reagent pouch 21 is sealed by means of thermoplastic sealing (heating time 30s); a...
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