Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Biochemical detection micro-fluidic chip, biochemical detection micro-fluidic chip system and applications thereof

A technology of microfluidic chip and biochemical detection, which is applied in the field of biochemical detection microfluidic chip system, biochemical detection, and biochemical detection microfluidic chip, which can solve the problem of difficulty in ensuring detection accuracy, lack of reagent storage function, Increase the risk of operation and other issues to achieve the effect of strong detection repeatability, avoid detection interference, and avoid cross-infection

Pending Publication Date: 2018-06-15
丁锐
View PDF6 Cites 12 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After patent research, it was found that there are a small number of related patents using microfluidic technology for biochemical detection at home and abroad in recent years. To drive, the chip needs to be rotated at a high speed, which increases the risk of operation; another example is a multi-index biochemical detection microfluidic chip disclosed in Chinese patent 201210142337, but the chip does not have the reagent storage function, and there is no error reporting mechanism , so it is difficult to guarantee the accuracy of the detection. At the same time, a pneumatic microvalve is used inside the chip to isolate the liquid, so as to realize the quantification of the liquid. However, this method is difficult to control accurately in practical applications, and it is difficult to achieve accurate quantification of the liquid in actual tests

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Biochemical detection micro-fluidic chip, biochemical detection micro-fluidic chip system and applications thereof
  • Biochemical detection micro-fluidic chip, biochemical detection micro-fluidic chip system and applications thereof
  • Biochemical detection micro-fluidic chip, biochemical detection micro-fluidic chip system and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0100] The preparation method of a preferred biochemical detection microfluidic chip provided by the present invention preferably includes the following steps (taking the chip including the liquid storage unit 2 as an example):

[0101] Step 1) prepack the reagents required for the biochemical reaction into the reagent pouch 21, and seal the sealing port 212 of the reagent pouch 21 by means of thermoplastic sealing; and place it in the chip system through the positioning hole 211 At the specific liquid storage structure of the middle structure, the reagent pouches 21 for each liquid storage are further fixed on the middle structure of the chip system by means of thermoplastic sealing.

[0102] Step 2) Different freeze-dried solid powders are placed inside each different reaction unit 3 in the middle layer structure of the chip, and the weight of the solid powder placed in each reaction unit 3 is constant.

[0103] Step 3) Bonding the lower structure to the lower part of the up...

Embodiment 1

[0117] This example is used to illustrate the preparation of the chip and chip system provided by the present invention and the multi-index detection of whole blood using the chip system

[0118] The biochemical indicators in whole blood to be detected in this embodiment are: alanine aminotransferase (ALT), aspartate aminotransferase (AST), γ-glutamyltransferase (γ-GT), alkali Phosphatase (ALP), total protein (TP), albumin (Alb), total cholesterol (TC), triglyceride (TG).

[0119] Various detection reagents and sample diluents to be used in this example were purchased from a Leadman Biochemical Reagent Company in Beijing. The processing method for each detection reagent is to obtain respective solid powders after freeze-drying each liquid reagent.

[0120] (1) Assembly of chip system

[0121] The above 300 μL diluent reagent is prepacked in the reagent pouch 21, and the sealing port 212 of the reagent pouch 21 is sealed by means of thermoplastic sealing (heating time 30s); a...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
thicknessaaaaaaaaaa
thicknessaaaaaaaaaa
diameteraaaaaaaaaa
Login to View More

Abstract

The invention relates to the technical field of biochemical detection of micro-fluidic chips and in particular relates to a biochemical detection micro-fluidic chip, a biochemical detection micro-fluidic chip system and applications thereof. The chip comprises a sampling unit (1), a reaction unit (3) and a waste liquid unit (4) and a first quantifying unit (5); the first quantifying unit (5) is used for quantifying a to-be-detected sample and is composed of a first slit (51) as well as a first sample chamber (52) and a second sample chamber (53) which are separated by the first slit (51); thefirst sample chamber communicates with the sampling unit, and the second sample chamber communicates with the waste liquid unit; and a third error reporting unit (14) is arranged between the reactionunit and the waste liquid unit and is provided with a chamber for receiving the to-be-detected sample from the reaction unit. The chip system is composed of three layers including an upper layer, a middle layer and a lower layer; the middle layer is the chip; the upper layer and the lower layer are used for covering and sealing the middle layer; and a sampling hole connected with the sampling unitand a demising hole corresponding to a liquid storage unit are formed in the upper layer. The chip is simple in detection process, capable of quantifying a reaction sample, high in sensitivity and strong in repeatability.

Description

technical field [0001] The invention relates to the technical field of biochemical detection of microfluidic chips, in particular to a biochemical detection microfluidic chip and a biochemical detection microfluidic chip system, and their application in biochemical detection. Background technique [0002] Microfluidic chip technology, also known as microfluidic chip lab or chip lab, refers to a chemical or biological laboratory built on a chip of a few square centimeters. The basic operation units such as sample preparation, reaction, separation, detection, cell culture, sorting, and lysis are integrated on a small chip, and a network of microchannels is formed to run through the entire system with controllable fluids to realize biological, chemical , various functions in fields such as medical diagnosis. [0003] The basic characteristics and biggest advantages of microfluidic chip technology are: a variety of unit structures can be flexibly combined on the tiny chip platf...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): B01L3/00G01N33/50
CPCG01N33/50B01L3/5027B01L2200/10B01L2200/027
Inventor 丁锐
Owner 丁锐
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products