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Use of oudemansiella spore dietary fiber extracts for preparing preparation for treating and/or preventing diseases associated with intestinal flora disorders

A technology for intestinal flora imbalance and dietary fiber, which is applied to health care products. In the pharmaceutical field, the dietary fiber extract of Ouddermis ooides can solve problems such as less research, achieve low operating costs, great economic and social value, The effect of inhibiting the expression of inflammatory factors

Inactive Publication Date: 2018-06-15
GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The present invention is aimed at the extract of Oudemansiellara Maflbes, which is an edible fungus with great nutrition, health care and medicinal value, and there are few studies on it at present

Method used

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  • Use of oudemansiella spore dietary fiber extracts for preparing preparation for treating and/or preventing diseases associated with intestinal flora disorders
  • Use of oudemansiella spore dietary fiber extracts for preparing preparation for treating and/or preventing diseases associated with intestinal flora disorders
  • Use of oudemansiella spore dietary fiber extracts for preparing preparation for treating and/or preventing diseases associated with intestinal flora disorders

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] The preparation method of the dietary fiber extract of Ouderia oosporus:

[0053] (1) After the sample is dried, crush and weigh it, add 95% ethanol at a material-to-liquid ratio of 1:10, boil and reflux with 95% ethanol for extraction for 2 hours, filter with suction, and retain the filter residue;

[0054] (2) Add water to the filter residue according to the material-to-liquid ratio of 1:10, boil and reflux for extraction for 2 hours;

[0055] (3) When the temperature drops to about 60°C, adjust the pH value to 6-7, add papain for enzymatic hydrolysis, the enzyme amount is 0.2% of the extract, enzymatically hydrolyze in a water bath at 60°C for 1 hour, and keep stirring during the period;

[0056] (4) After the enzymolysis is completed, add 95% ethanol in an amount 4 times the volume of the extract after enzymolysis to precipitate overnight, filter with suction, and retain the filter residue;

[0057] (5) The filter residue is gradient eluted with 78% ethanol, 95% et...

Embodiment 2

[0062] Example 2: The improvement effect of the dietary fiber extract of Ouderia oosporus on the intestinal tissue damage of obese mice induced by high-fat diet

[0063] 1. Reagents:

[0064] Total cholesterol (T-CHO) test kit: Nanjing Jiancheng Institute of Bioengineering.

[0065] Triglyceride (TG) test kit: Nanjing Jiancheng Institute of Bioengineering.

[0066] Low-density lipoprotein cholesterol (LDL-C) test kit: Nanjing Jiancheng Bioengineering Institute.

[0067] High-density lipoprotein cholesterol (HDL-C) test kit: Nanjing Jiancheng Bioengineering Institute.

[0068] Thirty female C57 mice, SPF grade, weighing 12-14 g, and 28-30 days old, were purchased from Guangdong Medical Experimental Animal Center with license number SCXK (Guangdong) 2013-0002.

[0069] Rats and mice maintained feed: Guangdong Medical Experimental Animal Center, license number SCXK (Guangdong) 2013-0002.

[0070] The high-fat and high-cholesterol feed formula is shown in Table 1:

[0071] Ta...

Embodiment 3

[0100] Example 3: The improvement effect of the dietary fiber extract of Ouderia oosporus on the diseases related to intestinal flora imbalance in obese mice induced by high-fat diet

[0101] 1. Experimental method

[0102] The feces of the mice at the end of the animal experiment in Example 2 were collected, quickly frozen with liquid nitrogen, and stored in a -80°C refrigerator. DNA was extracted from stool samples, and the quality of DNA extraction was detected by 0.8% agarose gel electrophoresis, and DNA was quantified by UV spectrophotometer. Forward primer 5'-ACTCCTACGGGAGGCAGCA-3' and reverse primer 5'-GGACTACHVGGGTWTCTAAT-3' were used to amplify the V3-V4 region gene of mouse 16S rRNA. The PCR amplification products were detected by 2% agarose gel electrophoresis, and the target fragments were recovered by cutting the gel, and the recovery was performed using the gel recovery kit of AXYGEN Company. Referring to the preliminary quantitative results of electrophoresis,...

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Abstract

The invention relates to the use of oudemansiella spore dietary fiber extracts in the pharmaceutical field, in particular to the use of the oudemansiella spore powder dietary fiber extracts for preparing a preparation for treating and / or preventing diseases associated with intestinal flora disorders. The oudemansiella spore powder dietary fiber extracts not only can obviously contribute to the structure recovery of dominant flora in intestinal tracts, contribute to the diversification of the flora, but also show obvious treatment and / or preventive effects on the diseases related to the structure of the intestinal flora; for example, the extracts can effectively inhibit the expression of inflammatory factors, effectively alleviate the deterioration of inflammation, improve various physiological and pathological indicators related to immunity, can be used in various fields such as medicines, health care products and the like, and have great economic and social value.

Description

technical field [0001] The present invention relates to the use of the dietary fiber extract of Ouderia oosporus in the fields of pharmacy, health care products, etc., and particularly relates to the use of the dietary fiber extract of Ouderia oodactana in the preparation of treatment and / or prevention of diseases related to intestinal flora imbalance use in formulations. Background technique [0002] In recent years, studies on gut microbiota have been emerging, and the role of gut microbiota in human health has attracted more and more attention. Gut flora is the normal microorganisms in the human intestinal tract. It is estimated that in a normal adult, the total weight of intestinal bacteria can reach 1-1.5kg. They can affect body weight and digestion, resist the risk of infection and autoimmune diseases, in addition, they are also associated with the occurrence of some endocrine and metabolic diseases, kidney diseases, neurological diseases, cardiovascular diseases, re...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/715A61P37/04A61P1/00A61P29/00C08B37/00A23L33/21
CPCA23L33/21A23V2002/00A61K31/715C08B37/0003A23V2200/32A23V2250/208
Inventor 陈地灵杨俭唐晓萃帅欧赖国校谢意珍吴清平杨柏华
Owner GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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