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Novel Torulaspora delbrueckii strain and application

A technology of extracting yeast and yeast powder, which is applied to a new strain of Saccharomyces spp. Delb and its application field, and can solve the problem of no detection of spice substances, etc.

Active Publication Date: 2018-06-08
WILMAR SHANGHAI BIOTECH RES & DEV CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In this report, Saccharomyces cerevisiae was only used to ferment beer, but no fragrance substances such as phenylethyl acetate, phenylethyl propionate, and butyric acid-β-phenylethyl ester were detected. In the optimal treatment, Saccharomyces cerevisiae and Spores of Spores Delbui was used at a ratio of 1:1, and only a maximum of 0.008mg / L of phenylethyl acetate was detected in the sample

Method used

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  • Novel Torulaspora delbrueckii strain and application
  • Novel Torulaspora delbrueckii strain and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] Embodiment 1: the isolation of bacterial strain

[0065] The soybean molasses samples collected from Yihai (Fangchenggang) Soybean Industry Co., Ltd. were collected and stored for strain isolation, and a colony was screened to obtain a spherical protrusion with a smooth, opaque and creamy surface (see figure 1 ). The microscopic observation of the thalline is round and contains spores in the Delbu spore yeast (see figure 2 ), its 18S rDNA base sequence is shown in SEQ: ID: NO: 1. Screening method refers to (Gao Yurong, Liu Yang et al. Screening and growth conditions of soybean molasses high-yielding single-cell protein strain [J]. China Brewing, 2010, 8:33-36).

[0066] The above-mentioned isolated non-engineering wild Torulaspora delbrueckii WBRD1.16083001 has been preserved in the General Microorganism Center of China Committee for Microbial Culture Collection (CGMCC, West Beichen Road, Chaoyang District, Beijing) on ​​October 21, 2016 No. 1, No. 3, Institute of M...

Embodiment 2

[0067] Embodiment 2: Activation of bacterial strain and preparation of seed solution

[0068] A. Activation of strains:

[0069] The isolated Saccharomyces delbui WBRD1.16083001 was diluted with sterile water and spread on the YPD solid plate medium, and cultured at a constant temperature of 28°C for 1-2 days, until the colonies on the plate were clear and easy to see.

[0070] The composition of YPD solid plate medium includes (unit 1L): yeast extract powder (OXOID Co., Ltd.) 10g, tryptone (OXOID Co., Ltd.) 20g, glucose (Sinopharm Chemical Reagent Co., Ltd.) 20g, agar powder (Aladdin Industry Co., Ltd.) Co., Ltd.) 10-20g, make up to 1L with distilled water, and the pH value is natural.

[0071] B. Preparation of seed solution:

[0072] Pick a large single colony from the above plate and inoculate it in liquid YPD medium, and culture it at 28°C and 220r / min for 20-24h. Bacterial concentration can reach 1-3×10 8 CFU / ml.

[0073] The composition of YPD liquid seed medium in...

Embodiment 3

[0074] Example 3: Analysis of volatile flavor substances in uninoculated YPD fermentation medium

[0075] YPD fermentation medium is the same as the YPD liquid seed medium described in embodiment 2.

[0076] Analysis of flavor components: directly analyze the YPD that was not inoculated with the wild Saccharomyces delbui of the present invention. The flavor components were analyzed by HS-SPMEGC-MS. GC-MAS detection method: GC-MS, Agilent 6890N / 5973Agilent (US company); gas chromatographic conditions: HP-5MS (30×0.25mm×0.25μm film thickness), programmed temperature rise: initial temperature 60°C, hold for 2min, Then rise to 150°C at 5°C / min, keep for 1min, then rise to 300°C at 5°C / min, and keep for 10min. The carrier is high-purity He. 1μl split 50:1, wherein, mass spectrometry conditions: interface temperature 280°C, EI source, ionization voltage 70eV, ion source temperature 230°C, scan range 29-400amu.

[0077] And through the built-in NIST spectral library to search the ...

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Abstract

The invention provides a novel Torulaspora delbrueckii strain and an application. The preservation number of Torulaspora delbrueckii is CGMCC NO.13125. The Torulaspora delbrueckii can be used for producing aroma substances, especially one or more of phenethyl acetate, 2-phenylethyl propionate and beta-phenethyl butyrate.

Description

technical field [0001] The invention relates to a new strain of Spores yeast delbu and its application. Background technique [0002] S. cerevisiae is a non-Saccharomyces cerevisiae. The colony shape is spherical protrusions with smooth, opaque and creamy surface. This type of yeast has the ability to withstand high osmotic pressure, can survive in grape juice with high sugar content, does not produce a large amount of acetic acid, and also has a strong ability to produce esters, improving the aroma of wine such as floral and fruity . For example, it is widely used in the brewing process of noble rot wine and ice wine. [0003] 2-Phenylethyl acetate, also known as phenylethyl acetate, phenylethyl acetate, benzyl methyl acetate, is a colorless liquid with a honey-sweet bottom-sweet rose aroma. It can be used as a substitute for methyl heptynoate in the preparation of scented soap and daily cosmetic essence. It is often used in the deployment of rose, orange blossom, viole...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/16C12P7/06C12P7/16C12P7/22C12P7/62C12R1/645
CPCC12P7/06C12P7/16C12P7/22C12P7/62C12P7/6436C12R2001/645C12N1/145Y02E50/10
Inventor 石兴利姜翠红宋鹏
Owner WILMAR SHANGHAI BIOTECH RES & DEV CENT
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