Method of inhibiting penicillium expansum and application of method
A technology to expand Penicillium and light intensity, applied in the direction of microorganism-based methods, biochemical equipment and methods, and treatment of microorganisms with electricity/wave energy, which can solve problems such as unsuitability for industrial applications, high processing costs, and radiation pollution. Achieve the effects of no radiation pollution risk, simple operation, and no secondary pollution
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Embodiment 1
[0048] The first step: Prepare PDA solid medium: glucose 20g, agar 15g, water 1000ml, weigh 200g of peeled potatoes and fresh potatoes according to the medium formula, cut the potatoes into small pieces and put them in the pot, add 1000ml of water, and heat on the heater Bring to a boil, keep it for 20-30 minutes, filter it on the measuring cup with 2 layers of gauze while it is hot, discard the filter residue, add water to the filtrate to 1000ml, heat to dissolve, put the filtrate into the pot, add 20g of glucose, 150g of agar, heat on low heat , and use a glass rod to stir continuously to prevent the agar from sticking to the bottom or overflowing. After the agar is completely dissolved, add water to the required amount. While hot, put 25ml of the prepared medium into a flat petri dish, and heat it at 121℃ Autoclaved, ready to use;
[0049] The second step: take out the Cerium sp. strains stored in the refrigerator at 4°C and inoculate them on the PDA solid medium;
[0050]...
Embodiment 2
[0052] Carry out the first step and the second step according to the method for embodiment 1, the difference is that the third step is implemented in this way: the PDA solid culture medium inoculated with the expanded Cerium strain is placed in an intelligent incubator to cultivate, and the blue light of the blue LED light Adjust the light intensity to 750lx, and irradiate at 37°C for 60 hours.
Embodiment 3
[0054] Carry out the first step and the second step according to the method for embodiment 1, the difference is that the third step is implemented in this way: the PDA solid culture medium inoculated with the expanded Cerium strain is placed in an intelligent incubator to cultivate, and the blue light of the blue LED light Adjust the light intensity to 1000lx, and irradiate at 20°C for 40 hours.
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