Mixed freeze-dried powder of platelet-rich plasma combined with excreted factor of human mesenchymal stem cells and preparation method thereof

Abstract

The invention discloses mixed freeze-dried powders of platelet-rich plasma combined with excreted factors of human mesenchymal stem cells and a preparation method thereof. The method comprises steps:preparing platelet-rich plasma; culturing excreted factor of mesenchymal stem cells; performing freeze-drying on the platelet-rich plasma and the excreted factor of mesenchymal stem cells, and mixingthe platelet-rich plasma and the excreted factor of mesenchymal stem cells, wherein weight percentage of self platelet-rich plasma contained in the mixed freeze-dried powders is larger than that of the excreted factor of mesenchymal stem cells. Autoblood and human cells are used as materials, to improve human absorption benefit and security, and a used nutrient solution is human platelet serum butnot animal fetal calf serum, to reduce protein variation. A freeze-drying technology is used to overcome a deterioration problem caused by heat sensitivity and aging, and meanwhile ratio of the mixedfreeze-dried powders can be customized, so as to be used in different environments later.

Description

technical field [0001] The present invention relates to a freeze-dried powder and its production method, and in particular, to a mixed freeze-dried powder combined with autologous platelet-rich plasma and human mesenchymal stem cell secretion factors and its production method. Background technique [0002] Platelet rich plasma (platelet rich plasma, PRP) contains a large number of biologically active platelets. Under certain conditions, these platelets can release various cytokines stored in it to the surrounding environment through the activation process to promote blood circulation. Cell proliferation, differentiation and tissue reconstruction and repair, therefore, it is a novel and convenient technology for treatment and medical aesthetics. [0003] Autologous platelet plasma technology can not only be applied to orthopedic treatment, but also has been widely used in the treatment of chronic wounds such as diabetic foot and decubitus. At the same time, autologous platele...

AI Technical Summary

Problems solved by technology

[0006] The clinical application of PRP and cytokines secreted by mesenchymal stem cells in medical aesthetics, anti-aging, and regeneration will be a future trend. The storage method is stored for subsequent use, resulting in the limitation of the frozen storage environment and high maintenance costs, and it must be thawed and mixed for human use during use, resulting in cumbersome and inconvenient subsequent use.

[0007] It can be seen that the above-mentioned conventional technology still has many deficiencies, it is not a good design, and needs to be improved urgently

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