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A strain of Lactobacillus plantarum ua-416 and its application

A technology of UA-416 and Lactobacillus plantarum, applied in the field of Lactobacillus plantarum UA-416 strain, can solve the problem of lactic acid bacteria reducing uric acid, etc., and achieve the effect of huge market space, broad application prospects and wide audience

Active Publication Date: 2019-01-01
JILIN MINGZHIYUAN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few reports about lactic acid bacteria reducing uric acid and anti-gout

Method used

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  • A strain of Lactobacillus plantarum ua-416 and its application
  • A strain of Lactobacillus plantarum ua-416 and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Embodiment 1 The preparation of plant lactobacillus (Lactobacillus plantarum) UA-416 strain of the present invention

[0051] 1. Isolation of Lactobacillus plantarum strains

[0052] 1. Sample material: traditional fermented food chili sauce.

[0053] Take 5g of solid hot sauce, add 45mL of normal saline (10-fold dilution) and mix well, take 0.5mL of diluent, and dilute to 10 times with normal saline again -3 , take 100μL liquid (10 -1 、10 -2 、10 -3 ) in solid BCP medium for cultivation (cultivation temperature 37°C, cultivation time 48-72h). Pick a single colony that produces a yellow circle and inoculate it on a solid MRS medium plate to continue culturing (cultivation temperature 37°C, culture time 48h), and repeatedly streak culture and purify on a solid MRS medium plate (cultivation temperature 37°C, culture time 48h) , to obtain multiple strains of pure culture strains.

[0054] 2. Inoculate the multiple strains of purely cultured strains obtained above into...

Embodiment 2

[0088] Example 2 Effect of plant lactobacillus (Lactobacillus plantarum) UA-416 strain of the present invention on hyperuricemia in mice induced by oxonic acid potassium salt

[0089] 1. Establishment and grouping of animal models

[0090] 60 male Kunming mice, weighing 22-25g, were randomly divided into 3 groups, 20 in each group, and were treated as follows: the model group and the UA-416 group were treated with canned stomach yeast extract (15g / kg) twice a day for 7 consecutive days, ethylamine Butanol (250mg / kg) once a day, the concentration of UA-416 group was 1.0×10 9 CFU / mL Lactobacillus plantarum UA-416 bacterial solution (12ml / kg body weight). 2 hours after the last administration, a one-time intraperitoneal injection of oxonate potassium (300 mg / kg). The blank control group was given the same dose of gastric or intraperitoneal injection of sterilized normal saline. After the experiment, the mice in each group were removed from the eyeballs to collect blood, centri...

Embodiment 3

[0096] Example 3 Preparation of fermented uric acid-lowering medicine and food homologous composition

[0097] 1. Preparation of Lactobacillus plantarum UA-416 starter

[0098] Cultivate Lactobacillus plantarum UA-416 to 20L, centrifuge at 6000r / min for 20min, resuspend in normal saline, centrifuge again at 6000r / min for 20min, resuspend and wash in normal saline, centrifuge again at 6000r / min for 20min, collect the sludge, dissolve in In 500mL of freeze-drying protectant, freeze-dry to get Lactobacillus plantarum UA-416 fermentation agent, the number of bacteria is about 1.0×10 9 CFU / g.

[0099] The formula of the lyoprotectant used: 90g of fructooligosaccharide and 10g of trehalose were dissolved in tertiary water, and the volume was adjusted to 500mL.

[0100] 2. Preparation of fermented uric acid-lowering medicine and food homologous composition

[0101] Mix 30 to 50 parts of corn silk, 20 to 40 parts of celery seed, 10 to 30 parts of mulberry, and 10 to 20 parts of ace...

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Abstract

A strain of Lactobacillus plantarum UA‑416 and its application belong to the field of functional food microorganisms and fill in the blank of Lactobacillus plantarum in reducing uric acid and anti-ventilation. A strain of Lactobacillus plantarum UA-416 of the present invention has been deposited in the China Center for Type Culture Collection on July 25, 2017, and the preservation number is: CCTCC NO: M2017419. The Lactobacillus plantarum UA‑416 strain of the present invention can effectively reduce blood uric acid levels, increase blood urea nitrogen and creatinine levels, inhibit xanthine oxidase activity in serum, and have preventive and therapeutic effects on uricemia or gout.

Description

technical field [0001] The invention belongs to the technical field of functional food microorganisms, and in particular relates to a strain of Lactobacillus plantarum UA-416 and an application thereof. Background technique [0002] Gout is a group of heterogeneous diseases that cause tissue damage caused by long-term purine metabolism disorders or elevated blood uric acid. The biochemical marker of gout is hyperuricemia, which refers to the supersaturated state of urate in the extracellular fluid. It is generally believed that hyperuricemia occurs when the blood uric acid is 416 μmol / L, and about 5-12% of patients with hyperuricemia will develop into hyperuricemia. gout. The acute attack of gout is an acute inflammatory reaction caused by the deposition of sodium urate in the form of crystals in joints and tissues around the joints. Gout can not only invade bones and joints, but also easily involve the kidneys and cardiovascular system. Hyperuricemia and primary gout are ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23L33/135A61K35/747A61P19/06C12R1/25
CPCA23L33/135C12N1/20A23V2002/00A61K2035/115C12R2001/25C12N1/205A23V2400/169A23V2200/30
Inventor 崔伟东梁铁
Owner JILIN MINGZHIYUAN BIOTECH
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