Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A method for identification of blast-resistant rice and a method for marking genes and its application

A technology of rice blast resistance gene and identification method, which is applied in the identification method of rice blast resistance rice and the marker method of genes and its application field, can solve the problems of poor accuracy and low selection efficiency of rice blast resistance genes, and speed up the progress of breeding Effect

Active Publication Date: 2021-04-13
云南省农业科学院生物技术与种质资源研究所
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Traditional field phenotyping has low selection efficiency and accuracy for rice blast resistance genes; an efficient breeding method for blast resistance rice is urgently needed

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A method for identification of blast-resistant rice and a method for marking genes and its application
  • A method for identification of blast-resistant rice and a method for marking genes and its application
  • A method for identification of blast-resistant rice and a method for marking genes and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] Example 1. Primer design and detection of rice blast resistance gene Pid2-specific CAPS marker Pid2caps

[0056] 1. Analysis of Pid2 gene sequence-specific SNP sites

[0057] The publicly published Pid2 gene and the sequenced Pid2 allelic sequences of rice varieties 93-11, Nipponbare and other 41 varieties were downloaded from public databases. SNPs for locus alleles. Such as figure 1 As mentioned above, the bases located at the +2057 and +2058 positions after the start codon of the Pid2 gene are "AT", and the genotypes of all other varieties have two types, the first type is the base "GC", and the second type is the base "GC". The base "GT". The SNP changes at these two sites just cause the Pid2 allele type of type 1 to be recognized by the restriction endonuclease Mlu1 at this position and cut at +2051, and the Pid2 allele type of type 2 can be cut at +2051. The position is recognized and cut at +2051 by the restriction endonuclease Fau1, but the Pid2 gene cannot ...

Embodiment 2

[0070] Example 2. Verification and application of rice blast resistance gene Pid2-specific CAPS marker in rice transfected with Pid2 gene coding region

[0071] (1) Using the rice variety Nipponbare that does not contain the rice blast resistance gene Pid2 as the transgenic recipient, the resistant rice

[0072] The CDS region of the blast gene Pid2 was highly expressed in the Nipponbare background, and the rice transfected with the coding region of the Pid2 gene was prepared.

[0073] (2), the genomic DNA of the transgenic rice and Nipponbare obtained in the extraction step 1 was used as a template respectively, and the primers in Example 1 were used for PCR amplification, and the obtained PCR products were digested with the restriction endonuclease Fau1 respectively, and the The digested products were detected by electrophoresis on agarose gel.

[0074] The result is as image 3 As shown, lane 1 is a non-transgenic plant of Nipponbare, which can be digested by Fau1, showin...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the field of crop molecular genetics and breeding, and specifically relates to a blast-resistant rice identification method and a gene marking method and application thereof, comprising the following steps: detecting the sequence of the Pid2 gene sequence FJ915121.1 in the No. 6 chromosome of the rice to be tested and The SNP site at the 5352nd and 5353th bases from the 5' end of its homologous sequence, through PCR amplification and restriction endonuclease digestion, the Pid2 gene sequence FJ915121.1 sequence and its homologous sequence in rice chromosome 6 The SNP types at the 5352nd and 5353rd bases from the 5' end were identified. The present invention can accurately determine whether the selected rice plants contain the rice blast resistance gene Pid2, and the method can be used to effectively select the rice blast resistance gene pid2 genotype, which can be used for identification and screening of rice resources, and can also be used for rice resistance Molecular breeding of the plague gene Pid2.

Description

technical field [0001] The invention belongs to the field of crop molecular genetics and breeding, and relates to a blast-resistant rice identification method, a gene marking method and an application thereof. Background technique [0002] Rice is an important food crop in the world, and rice blast is one of the most serious diseases in various rice regions in the world, seriously affecting the yield of rice. Due to the high variability and instability of Magnaporthe grisea, newly bred resistant varieties with a single resistance gene become susceptible varieties after a few years of popularization and planting, which has become an important obstacle to the sustainable development of rice production. Mining rice resistance gene resources is the basis of rice blast resistance breeding. Through extensive genetic analysis, 84 rice blast resistance genes have been identified and mapped, and at least 24 major genes have been isolated and cloned. With the widespread application ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/686C12Q1/6895C12N15/11
CPCC12Q1/686C12Q1/6895C12Q2600/156C12Q2521/301
Inventor 孙一丁许明辉马继琼杨奕李进斌
Owner 云南省农业科学院生物技术与种质资源研究所
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com