Method for homogenously detecting exon mutation indexes of EGFR gene 21

Abstract

The invention relates to a method for homogenously detecting exon mutation indexes of an EGFR gene 21, and the method is suitable for a fluorescence polarization detection technology. The method comprises the following steps: separately detecting a negative control standard substance, a positive control standard substance as well as FAM of a final amplification product of an exon target gene of ato-be-detected sample EGFR allele 21 and a R110 fluorescence polarization value, and calculating an exon mutation index of the to-be-detected sample EGFR allele 21. The invention creatively disclosesthe method for detecting the exon mutation index of the EGFR allele 21 and the exon mutation index of the to-be-detected sample EGFR allele 21, namely a method for detecting a proportion of exon anomaly to a exon wild type of the EGFR allele in a genome, wherein the exon mutation index of the EGFR allele 21 is a molecular marker which is highly correlated with drug responsiveness relative to absolute quantity of exon anomaly of the EGFR allele 21 and is more sensitive and specific. Detection for the exon mutation index of the EGFR allele 21 is more scientific, accurate and standard for detection for variation type and absolute quantity, and has a better predication value.

Description

technical field [0001] The invention relates to the technical field of detection of mutation index of exon 21 of EGFR allele, in particular to a method for homogeneous detection of mutation index of exon 21 of EGFR gene. Background technique [0002] Allelic variation is the variation of a gene that controls a certain trait at the same position in the double-stranded DNA of a pair of homologous chromosomes in a cell. In the individual genome, allelic variation and wild type exist at the same time, mutually restricting the balance, affecting gene expression and function together, affecting biological responses, and determining phenotypes such as cell fate; allelic variation within individuals has heterogeneity and dosage Effect, different individuals have different relative quantitative levels of allelic variation and wild type at different stages, and the relative quantitative level of variation directly affects gene function and expression. EGFR allelic exon 21 variation a...

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to provide a method for homogeneous detection of EGFR gene exon 21 mutation index, which is suitable for fluorescence polarization detection technology, and is used for EGFR allele 21 exon mutation index, that is, EGFR allele 21 exon The detection of the content ratio of wild type and mutant type overcomes the problems of one-sidedness, high cost, cumbersome operation steps and easy pollution in the prior art

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