Method for homogeneously detecting mutation index of exon of EGFR allele 19

Abstract

The invention relates to a method for homogeneously detecting a mutation index of an exon of an EGFR allele 19. The method comprises the following steps: amplifying a specific reagent; then performing FAM and R110 fluorescence polarization value detection on an amplified product; and judging the mutation index of the exon of the EGFR allele 19 of a to-be-detected specimen according to fluctuation and specific value of FAM and R110 fluorescence polarization values. The invention creatively provides the method for detecting the mutation index of the exon of EGFR allele 19 and the mutation index of the exon of the EGFR allele 19. The mutation index of the exon of EGFR allele 19 is a molecular identifier which is highly related to and more sensitive and specific to drug reactivity compared with absolute quantity and anomaly of the exon of EGFR allele 19. The method for detecting the mutation index of the exon of the EGFR allele 19 is more scientific and accurate compared with that of detecting anomaly and absolute quantity only, and the method performs detection of criterion and quantity and with a predictive value.

Description

technical field [0001] The invention relates to the technical field of detection of EGFR allele exon 19 mutation index, in particular to a method for homogeneously detecting the EGFR allele exon 19 mutation index. Background technique [0002] Allelic variation is the variation of a gene that controls a certain trait at the same position in the double-stranded DNA of a pair of homologous chromosomes in a cell. In the individual genome, allelic variation and wild type exist at the same time, mutually restricting the balance, affecting gene expression and function together, affecting biological responses, and determining phenotypes such as cell fate; allelic variation within individuals has heterogeneity and dosage Effect, different individuals have different relative quantitative levels of allelic variation and wild type at different stages, and the relative quantitative level of variation directly affects gene function and expression. EGFR allelic exon 19 variation and wild...

AI Technical Summary

Problems solved by technology

[0003] The object of the present invention is to provide a method for homogeneous detection of EGFR allele 19 exon mutation index by fluorescence polarization, which is used for EGFR allele 19 exon mutation index, that is, EGFR allele 19 exon wild type and The detection of the content ratio of the mutant type overcomes the problems of one-sidedness, high cost, cumbersome operation steps and easy pollution in the prior art