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Active substance of cordyceps cicadae/sobolifera and use thereof for reducing intraocular pressure

An active substance, the technology of cicadae, which is applied in the field of cicadae active substances and compositions containing it, can solve the problems of few natural cicadae fruiting bodies, restrictions on large-scale use, and reduction of natural Cordyceps sinensis production, etc.

Active Publication Date: 2018-05-04
GRAPE KING
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

And the output of natural Cordyceps sinensis is decreasing day by day, and there are not many natural cicadae fruiting bodies, which limit a large amount of use

Method used

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  • Active substance of cordyceps cicadae/sobolifera and use thereof for reducing intraocular pressure
  • Active substance of cordyceps cicadae/sobolifera and use thereof for reducing intraocular pressure
  • Active substance of cordyceps cicadae/sobolifera and use thereof for reducing intraocular pressure

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0065] Preparation of Active Substances of Cicada Flower

[0066] Cicada mycelium source

[0067] The used cicadae (Cordyceps cicadae) mycelium of the embodiment of the present invention is obtained by collecting the wild cicadae fruiting body of Taiwan, China, and obtains its mycelium through separation, and subculture is preserved on the plate culture medium, passed through Taiwan, China The identification of the Food Industry Development Research Institute confirmed that its gene sequence is Cordyceps cicadae. Deposited in the General Microbiology Center of China Committee for Culture Collection of Microorganisms, No. CGMCC No.10486). But the cicadae active substance described in the present invention is not limited to obtain by this strain.

[0068] liquid culture

[0069] Liquid fermentation culture can have different degrees of control equipment according to the scale, including shake flasks, tanks, stirring control, temperature control, pH control, dissolved oxygen c...

Embodiment 1

[0078] Cicada flower liquid fermentation culture and preparation of active substances

[0079] Mycelium strain: BCRC MU30106

[0080] Plate culture: inoculate the mycelium of cicadae on a plate medium, the medium is potato dextrin medium (Potato Dextrose Agar, PDA), and cultivate it at 25° C. for about 5 days.

[0081] Flask culture: Scrape the mycelia on the plate and inoculate into the flask, use the culture medium in Table 2, at about 25°C, pH 4.5, shake and culture on a shaker at a speed of 120rpm for 3 days.

[0082] Table 2 medium formula

[0083] Element

Content (weight%)

sucrose

2.0

yeast extract

0.5

soy flour

1.0

[0084] Fermentation tank culture: the culture medium is the same as in Table 2, inoculate the flask culture into the fermentation tank culture medium, at 25°C, the tank pressure is 0.5-1.0kg / cm 2, pH 4.5, 10-150rpm stirring speed or no stirring (air lift) situation, feed air with 0.5-1.0VVM aeration rate...

Embodiment 2

[0088] Analysis of Animal Models and Correlative Indicators for Lowering IOP

[0089] experimental animals

[0090] The experimental animals are adult, 8-12 weeks old, female New Zealand White rabbits (New Zealand White (NZW) Rabbit), the test number is marked with the ear to distinguish the individual experimental animals, and the cage number, species, and week are marked on the breeding cage. Age, animal test number, test group, entry date and test period. The light time in the breeding area is automatically controlled to be 12 hours bright and 12 hours dark, the room temperature is 23±2°C, and the relative humidity is 40-70%. Animals had free access to adequate food and drinking water. During the period of quarantine and experiment, the clinical symptoms of experimental animals were observed and recorded by veterinarians and experimenters respectively to ensure the health status of experimental animals. Experiments can only begin after the experimental animals have been ...

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Abstract

The invention provides an active substance of cordyceps cicadae / sobolifera and the use thereof for reducing intraocular pressure. The active substance of cordyceps cicadae / sobolifera is prepared by the steps of (a) culturing the cordyceps cicadae / sobolifera mycelium on a flat plate culture medium at 15 to 35 DEG C for 5 to 14 days; (b) inoculating the cordyceps cicadae / sobolifera mycelium culturedin the step (a) into a flask to be cultured at 15 to 35 DEG C and pH 2 to 8 for 3 to 10 days; (c) inoculating the cordyceps cicadae / sobolifera mycelium cultured in the step (b) into a fermentation tank to be cultured at the conditions of 15 to 35 DEG C and pH 2 to 8 for 3 to 10 days to form a cordyceps cicadae / sobolifera mycelium fermentation liquid; (d) freeze-drying the cordyceps cicadae / sobolifera mycelium fermentation liquid, then grinding into powder to form a cordyceps cicadae / sobolifera mycelium freeze-dried powder; and (e) performing solvent extraction on the cordyceps cicadae / sobolifera mycelium freeze-dried powder to form a cordyceps cicadae / sobolifera mycelium extract containing the active substance of cordyceps cicadae / sobolifera.

Description

technical field [0001] The present invention relates to a cicadae active substance and a composition containing it, in particular to the use of the cicadae active substance and its composition for lowering intraocular pressure. Background technique [0002] Aqueous humor [0003] Eyes are made of soft yet strong tissue that allows for light and rapid movements. Aqueous humor circulates in the eyes, carrying oxygen, sugar, and other essential nutrients needed to nourish the eyes. The composition of aqueous humor is related to the metabolites produced by the tissues that flow through it. For example, the lens body consumes glucose, potassium ions, and amino acids in the aqueous humor to produce metabolites of lactic acid; the cornea is similar, and its metabolism will also consume Nutrients supplied by water. Therefore, the aqueous humor in the anterior chamber contains a lower concentration of glucose and a higher concentration of lactic acid than the posterior chamber of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/066A61P27/02A61P27/06A23L31/00A23L33/105C12N1/14A61K35/64
CPCA23V2002/00A61K36/066A61K2236/19A61K35/63C12N1/14A23V2200/30A23V2250/208
Inventor 陈劲初叶淑幸李丽雅徐瑞霞陈彦博
Owner GRAPE KING
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