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Phaseolin polypeptide preparation method and obtained phaseolin polypeptide

A protein polypeptide and conditional technology, which is applied in the field of protein polypeptide and protein polypeptide preparation, can solve the problems of uncontrollable hydrolysis degree, unfavorable absorption and utilization, amino acid destruction, etc., and achieve the effect of short preparation time, easy control and small molecular weight

Inactive Publication Date: 2018-04-20
GUANGDONG ZHENGDANGNIAN BIO TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Oysters, leeches, and deer blood are all very precious medicinal raw materials. Currently, there are a lot of macromolecular proteins in health care products, which are not conducive to the absorption and utilization of the human body, and greatly reduce the efficacy of the medicine.
In order to make the drug easier for the human body to absorb, acid, alkali or enzymes are generally used to hydrolyze macromolecular proteins. The acid-base hydrolysis is more thorough and fast, but the degree of hydrolysis cannot be controlled, and it will cause damage to some amino acids.
Although enzymatic hydrolysis will not destroy amino acids, the current complex protein hydrolysis takes a long time, and the content of oligopeptides after hydrolysis is low, which cannot meet the needs of modern society.

Method used

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  • Phaseolin polypeptide preparation method and obtained phaseolin polypeptide
  • Phaseolin polypeptide preparation method and obtained phaseolin polypeptide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Embodiment 1, the preparation of a kind of protein polypeptide

[0033] Step 1. Grinding 10 parts of oysters, 15 parts of deer blood, 6 parts of leeches and 30 parts of water to obtain a mixed slurry;

[0034] Step 2, pass AC current into the mixed solution obtained in Step 1, the frequency of the AC current is 8200Hz, the voltage is 2000V, process for 8min, cut off the power supply, then after 20min by constant voltage reverse current treatment, the constant voltage The voltage of the reverse current is 500V, and the constant voltage forward current is passed through for 16min, and the voltage of the constant voltage forward current is 500V to obtain the mixed solution I;

[0035] Step 3, place the mixed solution I obtained in step 1 at room temperature and stir for 20min, then add elastase, the addition of the elastase is 0.3‰ of the total weight of the mixed solution I, and after 1h under neutral greenhouse conditions, heat up to Inactivate the enzyme at 75°C, centr...

Embodiment 2

[0038] Embodiment 2, the preparation of a kind of protein polypeptide

[0039] Step 1, grinding 16 parts of oysters, 15 parts of deer blood, 8 parts of leeches and 35 parts of water to obtain a mixed slurry;

[0040] Step 2, pass AC current into the mixed solution obtained in Step 1, the frequency of the AC current is 9000Hz, the voltage is 2500V, process for 6min, cut off the power supply, then after 15min by constant voltage reverse current treatment, the constant voltage The voltage of the reverse current is 530V, and the constant voltage forward current is passed through for 10min, and the voltage of the constant voltage forward current is 530V to obtain the mixed solution I;

[0041] Step 3, place the mixed solution I obtained in step 1 at room temperature and stir for 25 minutes, then add elastase, the amount of elastase added is 0.5‰ of the total weight of the mixed solution I, after 1.5h under neutral greenhouse conditions, heat up Inactivate the enzyme at 90°C, centr...

Embodiment 3

[0044] Embodiment 3, the preparation of a kind of protein polypeptide

[0045] Step 1, grinding 20 parts of oysters, 15 parts of deer blood, 10 parts of leeches and 40 parts of water to obtain a mixed slurry;

[0046] Step 2, pass AC current into the mixed solution obtained in Step 1, the frequency of the AC current is 9500Hz, the voltage is 3000V, process for 4min, cut off the power supply, then after 10min by constant voltage reverse current treatment, the constant voltage The voltage of the reverse current is 600V, and the constant voltage forward current is passed through for 16 minutes, and the voltage of the constant voltage forward current is 600V to obtain the mixed solution I;

[0047] Step 3, place the mixed solution I obtained in step 1 at room temperature and stir for 30 minutes, then add elastase, the addition of the elastase is 0.8‰ of the total weight of the mixed solution I, and after 2 hours under neutral greenhouse conditions, heat up to Inactivate the enzyme ...

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Abstract

The invention relates to the technical field of biological engineering and in particular to a phaseolin polypeptide preparation method and obtained phaseolin polypeptide. The phaseolin polypeptide preparation method provided by the invention comprises the following steps: performing cell and protein degradation with current, and performing enzymolysis with elastin, thereby obtaining phaseolin polypeptide of which the molecular weight is less than 1000Da. The phaseolin polypeptide preparation method provided by the invention has the advantages of being simple in process, easy to control and short in preparation cycle, and the prepared phaseolin polypeptide is high in yield and high in purity. In addition, the phaseolin polypeptide of which the molecular weight is less than 1000Da, which isprepared by using the method, has a remarkable improvement function on male sexual functions, and benefits can be made for massive males.

Description

technical field [0001] The invention relates to the technical field of bioengineering, in particular to a method for preparing a protein polypeptide and the obtained protein polypeptide. Background technique [0002] Oyster, also known as oyster, oyster yellow, sea oyster, is a kind of bivalve mollusk, belonging to the mollusk phylum, valve branch class, oyster family. Oyster is the largest cultured shellfish in the world, mainly distributed along the coasts of temperate and tropical oceans, and it is also one of the four major cultured shellfish in my country. "Supplements to Materia Medica" records that peony has the effect of "cooking and eating raw in ginger vinegar, controlling erysipelas, irritability after drinking, and quenching thirst". However, due to the strong production season of oysters and strong fishy smell, its application fields are limited. [0003] Leeches, also known as leeches, are a traditional Chinese medicine with a long history. Chinese medicine b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K2/00C07K1/34
CPCC07K2/00C12P21/06
Inventor 江小元
Owner GUANGDONG ZHENGDANGNIAN BIO TECH CO LTD
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