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Nanometer medicine for controlled photodynamic treatment and preparation method thereof

A technology of photodynamic therapy and nano-medicine, applied in the field of biomedicine, can solve the problem of high dark toxicity of hematoporphyrin, achieve the effects of improving therapeutic effect, improving solubility, and solving dark toxicity

Active Publication Date: 2018-04-20
FUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The object of the present invention is to provide a controllable preparation method and application of nano drug CPHI with low dark toxicity. Using the function of ICG switch, it not only solves the problem of high dark toxicity of hematoporphyrin, but also realizes controllable Combination of photodynamic therapy and photothermal therapy

Method used

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  • Nanometer medicine for controlled photodynamic treatment and preparation method thereof
  • Nanometer medicine for controlled photodynamic treatment and preparation method thereof
  • Nanometer medicine for controlled photodynamic treatment and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Weigh β-CD (126.4 mg) and CDI (180.56 mg) and dissolve them in 7 mL of DMSO. After fully stirring the reaction for 6 h, the fifth-generation polyamide-amine dendrimer (G5 PAMAM) (115.9 mg) in DMSO solution (3 mL) was added dropwise to the solution, and stirred at room temperature for 3 days. After the reaction is over, the reaction solution is transferred to a dialysis bag with a molecular weight cut-off of 8000-14000 Da, and dialyzed in secondary water for 3 days. Finally, freeze-dried to obtain a white powdery product (CP), stored at -20°C. Use deuterated DMSO as 1 Test solvent for H-NMR spectrum, from figure 1 It can be seen that cyclodextrin has been successfully coupled to PAMAM.

Embodiment 2

[0043] Take the aqueous solution of CP, ICG, HP to measure the UV absorption, such as figure 2 As shown, HP has characteristic ultraviolet absorption peaks at 374 nm and 500-620 nm, ICG has characteristic ultraviolet absorption peaks at 778 nm, and CP has no ultraviolet absorption peaks at 300-900 nm. Measure the fluorescence intensity of CP, ICG, HP at an excitation wavelength of 401 nm, such as image 3 The HP shown has characteristic peaks of HP at 616nm and 666nm, while CP and CPI have no fluorescence.

Embodiment 3

[0045] Weigh 10mg CP and dissolve it in 8mL DMSO, add ICG (2mg, 1mL DMSO) and HP (0.2mg, 1mL DMSO) dropwise to the CP solution, and stir for 3 days at room temperature and dark. The reaction solution was dialyzed in secondary water for 3 days and freeze-dried to obtain CPHI10. Among them, the encapsulation rate and loading rate of ICG are 15.4%±0.63% and 6.16%±0.03%, respectively, and the encapsulation rate and loading rate of HP are 82.5%±0.25% and 3.30%±0.64%, respectively.

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Abstract

The invention discloses polyamidoamine-dendrimers (PAMAM-CD) / HP(haematoporphyrin) / ICG(indocyanine green) nanometer medicine with controllable photodynamic treatment effects, and a preparation method thereof. The nanometer medicine is characterized in that the HP and the ICG are coated and loaded into PAMAM-CD; the ICG is used as a switch of the HP; the fluorescence of the HP is quenched by ICG; meanwhile, the photodynamic treatment is switched off. When the nanometer medicine is exposed in 808nm laser, the ICG is decomposed; the HP fluorescence is restored; the photodynamic treatment is opened; meanwhile, the ICG photothermal treatment is accompanied. The medicine solves the problem of dark toxicity of the HP, and also realizes the combination of the controlled photodynamic treatment and the photothermal therapy through the switch effect of the ICG.

Description

Technical field [0001] The invention belongs to the field of biomedicine, and relates to the preparation and application of a novel and controllable photodynamic therapy drug PAMAM / hemoporphyrin / indocyanine green (CPHI). Background technique [0002] Photodynamic therapy (PDT) as a minimally invasive treatment has been applied to the treatment of various cancers. The process is that under a specific wavelength of laser light, the photosensitizer molecules (PSs) in cancer cells are excited, transfer energy to the surrounding oxygen, and generate highly toxic reactive oxygen species (ROS), thereby killing tumor cells. Compared with conventional chemotherapy, invasive surgery and radiotherapy, PDT is characterized by being selective for tumor cells, leading to tumor cell necrosis or apoptosis, and no damage to surrounding normal cells and tissues (Kessel D, Luo Y. Mitochondrial photodamage and PDT) -induced apoptosis[J].Journal of Photochemistry & Photobiology B Biology, 1998, 42(2...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K41/00A61K47/69A61K47/61A61P35/00
CPCA61K41/0052A61K41/0071A61K2300/00
Inventor 高瑜李子颖吕婷婷王秀英阙榕槟马士杰陈海军
Owner FUZHOU UNIV
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