Tamoxifen treatment effect-related gene (CYP2D6, CYP3A5 and RRAS2) polymorphism detection kit and tamoxifen treatment effect-related gene (CYP2D6, CYP3A5 and RRAS2) polymorphism detection method

A gene polymorphism and detection kit technology, applied in the fields of biotechnology and medicine, can solve problems such as high price and reduce pollution

Inactive Publication Date: 2018-03-27
宁波美丽人生医药生物科技发展有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, related kits on the market includ

Method used

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  • Tamoxifen treatment effect-related gene (CYP2D6, CYP3A5 and RRAS2) polymorphism detection kit and tamoxifen treatment effect-related gene (CYP2D6, CYP3A5 and RRAS2) polymorphism detection method
  • Tamoxifen treatment effect-related gene (CYP2D6, CYP3A5 and RRAS2) polymorphism detection kit and tamoxifen treatment effect-related gene (CYP2D6, CYP3A5 and RRAS2) polymorphism detection method
  • Tamoxifen treatment effect-related gene (CYP2D6, CYP3A5 and RRAS2) polymorphism detection kit and tamoxifen treatment effect-related gene (CYP2D6, CYP3A5 and RRAS2) polymorphism detection method

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Experimental program
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Embodiment 1

[0031] 1. Sample Processing and Nucleic Acid Extraction

[0032] Use a commercial DNA extraction kit to process the sample. For specific operations, refer to the kit instruction manual. The concentration and purity of the extracted DNA need to be measured with a UV spectrophotometer. The DNA OD 260 / OD 280 The value should be between 1.8 and 2.0, and the concentration should be between 5 and 50 ng / μL. If the sample DNA quality is unqualified, it should not be used for testing. It is recommended that those whose DNA quality is lower than 5 ng / μL be re-taken for nucleic acid extraction, and those higher than 50 ng / μL Properly dilute to the specified concentration range. It is recommended to test the extracted DNA immediately, otherwise, please store it below -20°C, and the storage time should not exceed 6 months. The quality control product of the kit was thawed at room temperature before use, vortexed for 10 seconds, and centrifuged at 2000rpm for 15 seconds before use.

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Abstract

The invention provides a drug tamoxifen treatment effect-related gene (CYP2D6, CYP3A5 and RRAS2) polymorphism detection kit and a drug tamoxifen treatment effect-related gene (CYP2D6, CYP3A5 and RRAS2) polymorphism detection method, wherein the kit comprises a PCR buffer solution, specific ARMS detection primers and quality control primers. The present invention further provides the tamoxifen treatment effect-related gene (CYP2D6, CYP3A5 and RRAS2) polymorphism detection method. According to the present invention, with the method, the tamoxifen treatment effect-related gene (CYP2D6, CYP3A5 andRRAS2) polymorphism can be rapidly and accurately detected; and the kit has characteristics of high sensitivity, strong specificity, simple method, accurate result and the like.

Description

Technical field: [0001] The invention relates to the fields of biotechnology and medicine, in particular to a detection kit and a detection method for gene polymorphisms of tamoxifen curative effect related genes (CYP2D6, CYP3A5 and RRAS2). Background technique: [0002] Adjuvant endocrine therapy is an important way in the comprehensive treatment of breast cancer. Breast cancer chemotherapy is closely related to hormone levels in the body. Tamoxifen (TAM), also known as tamoxifen, is a synthetic non-cholesterol anti-estrogen drug and is the basic drug for endocrine adjuvant therapy for breast cancer. After TAM binds to estrogen receptors, it induces changes in the three-dimensional spatial configuration of the receptors, prevents the receptors from combining with estrogen-related reaction components on DNA, prevents normal processes such as transcription, prevents estrogen from functioning, and inhibits hormone dependence. The growth of sexual mammary tumor cells achieves ...

Claims

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Application Information

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IPC IPC(8): C12Q1/6876C12Q1/6858
CPCC12Q1/6858C12Q1/6876C12Q2600/106C12Q2600/156C12Q2600/16C12Q2535/137C12Q2563/107
Inventor 田晓丽
Owner 宁波美丽人生医药生物科技发展有限公司
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