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Two-phase aqueous extraction combined with forward osmosis condensation and extraction technology for blueberry leaf anthocyanidin

A forward osmosis and anthocyanin technology, applied in solvent extraction, organic chemistry, chemical instruments and methods, etc., can solve the problems of anthocyanin waste, low extraction rate degradation rate, anthocyanin degradation, etc., to improve extraction Efficiency, the effect of reducing extraction time

Active Publication Date: 2018-03-27
菏泽天蓝果蔬专业合作社
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the extraction time of this method is short, the extraction rate of anthocyanins in blueberries is low
The extraction rate of anthocyanins is low, which will cause waste of anthocyanins
When the extraction time is too long, it is easy to cause the degradation of anthocyanin, which is not conducive to the improvement of the extraction rate of anthocyanin, and is not conducive to improving the purity of the anthocyanin product, so there is an urgent need for a high extraction rate, low degradation rate, high Extraction method for purity

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] A blueberry leaf anthocyanin two-phase extraction combined with forward osmosis concentration extraction technology, comprising the following steps:

[0041] 1) Grind the dried blueberry leaves to 200 mesh, weigh 1g of blueberry leaf powder, mix and extract the blueberry leaf powder with 2ml of water to obtain a crude extract of anthocyanins, add ascorbic acid to the crude extract to increase its mass The concentration is 0.2%, inhibiting the activity of polyphenol oxidase;

[0042] 2) Add polyethylene glycol and magnesium chloride to the crude extract prepared in step 2), the mass ratio of polyethylene glycol, magnesium chloride and crude extract is 3:2:15, mix and extract for 1 hour, after equilibrium, separate the top phase and the bottom phase, and the separated top phase was repeatedly extracted 3 times with the two-phase extraction system to remove the sugar in the crude extract, and then the polyethylene glycol in the top phase was removed by chloroform extractio...

Embodiment 2

[0047] A blueberry leaf anthocyanin two-phase extraction combined with forward osmosis concentration extraction technology, comprising the following steps:

[0048]1) Grind the dried blueberry leaves to 100 mesh, weigh 1g of blueberry leaf powder, mix and extract the blueberry leaf powder with 3ml of water to obtain a crude extract of anthocyanins, add TBA to the crude extract to increase the mass The concentration is 0.3%, inhibiting the activity of polyphenol oxidase;

[0049] 2) Add polyethylene glycol and magnesium chloride to the crude extract prepared in step 2), the mass ratio of polyethylene glycol, magnesium chloride and crude extract is 3:2:16, mix and extract for 1 hour, after equilibrium, separate the top phase and the bottom phase, and the separated top phase was extracted twice using the two-phase extraction system to remove the sugar in the crude extract, and then the polyethylene glycol in the top phase was removed by chloroform extraction to obtain a mixture ...

Embodiment 3

[0054] A blueberry leaf anthocyanin two-phase extraction combined with forward osmosis concentration extraction technology, comprising the following steps:

[0055] 1) Crush the dried blueberry leaves to 100 mesh, weigh 1g of blueberry leaf powder, mix and extract the blueberry leaf powder with 2ml of water to obtain a crude extract of anthocyanins, add cysteine ​​to the crude extract, Make its mass concentration 0.3%, inhibit the activity of polyphenol oxidase;

[0056] 2) Add polyethylene glycol and magnesium chloride to the crude extract prepared in step 2), the mass ratio of polyethylene glycol, magnesium chloride and the crude extract is 3.5:2:16, mix and extract for 1 hour, after equilibrium, separate the top phase and the bottom phase, and the separated top phase was extracted twice using the two-phase extraction system to remove the sugar in the crude extract, and then the polyethylene glycol in the top phase was removed by chloroform extraction to obtain a mixture li...

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PUM

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Abstract

The invention discloses two-phase aqueous extraction combined with forward osmosis condensation and extraction technology for blueberry leaf anthocyanidin. The technology comprises the following steps: (1) dried blueberry leaves are crushed, blueberry leaves and an extraction solvent are mixed for extraction, a crude extract of anthocyanidin is obtained, and a polyphenol oxidase active inhibitor is added into the crude extract; (2) polyethylene glycol and magnesium chloride are added into the crude extract prepared in the step (2), mixed extraction time is set, after balancing, a top phase anda bottom phase are separated, a two-phase aqueous extraction system is used for carrying out multitime repeat extraction of the top phase obtained by separation in order to remove sugar in the crudeextract, polyethylene glycol in the top phase is removed, and mixed liquor is obtained; (3) membrane permeation distillation and condensation is carried out for the mixed liquor in the step (2), a penetration solution is a calcium chloride solution, the volume ratio of material liquid to the penetration solution is 1:3-5, and the material liquid is the mixed liquor in the step (2); (4) an ultrafiltration method is used for clarifying the crude extract product of anthocyanidin, and a forward osmosis solution is a sodium chloride solution.

Description

technical field [0001] The technical field of extraction of active components in blueberry leaves of the present invention specifically relates to a blueberry leaf anthocyanin two-phase extraction combined with forward osmosis concentration extraction technology. Background technique [0002] Blueberry is a shrub, with 25-30 fruiting branches per clump. It blooms and bears fruit every 2 years, but the yield is small. There is a proper yield in the 3rd year, and it enters the high yield period in the 5th year. Its life span is 50 years. Anthocyanin is a pure natural anti-aging nutritional supplement. Studies have shown that it is the most effective antioxidant found by humans today. Its antioxidant performance is 50 times higher than vitamin E and 200 times higher than vitamin C. It is 100% bioavailable to the human body and can be detected in the blood twenty minutes after ingestion. Anthocyanins are known as "oral skin cosmetics" in Europe, especially blueberry anthocyanin...

Claims

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Application Information

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IPC IPC(8): C07D311/62
CPCC07D311/62B01D11/0288
Inventor 孙锐孙蕾白瑞亮韩晓光解小锋林相恺李雪彤
Owner 菏泽天蓝果蔬专业合作社
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