Tissue culture breeding method of cold-resistant rose

Abstract

The invention relates to a tissue-culture propagation method of cold-resistant Chinese rose and solves the propagation problems of slow propagation and low multiplication coefficient of the cold-resistant Chinese rose. The method includes the steps of firstly, screening explants; secondly, sterilizing the explants; thirdly, performing primary culture; fourthly, performing multiplication culture; fifthly, performing strong-seedling culture; sixthly, performing rooting culture; seventhly, transplanting and performing acclimation. The method is characterized in that newly grown top buds or lateral buds of plants are used as the explants, MS is used as the basic culture medium, naphthylacetic acid (NAA), 6-benzylamino purine (6BA) and indolebutyric acid (IBA) are utilized to perform hormonal regulation, and accordingly the regeneration system of the cold-resistant Chinese rose is built. The method has the advantages that one multiplication subculture cycle of the method is 4-5 weeks, multiplication times is controlled to be 6-8 times, the method is unaffected by seasons, seedling propagation coefficient can be increased greatly, a scientific and effective propagation method is providedfor the cultivation and application of the cold-resistance Chinese rose, production requirements are satisfied, industrial propagation can be performed, and reference can be provided for the propagation of other cold-resistant Chinese rose varieties.

Description

technical field [0001] The invention relates to a method for tissue culture and breeding of cold-resistant roses. Background technique [0002] The present invention relates to a cold-resistant rose which belongs to the genus Rosaceae (Rosaceae) Rosa L. ) Perennial woody flowers, thorny shrubs. Leaves alternate, odd-pinnate compound leaves. The flowers are solitary or in panicles, with many petals, rich and bright colors, and a long flowering period. In addition to being used as cut flowers, it is also an important material for flower beds, flower borders, foundation planting and flower hedges. It is often found in lawns, gardens, park green spaces, etc. Planting is widely used in landscaping. [0003] However, due to the cold and long winter in Northeast China and the short growing season in summer, there are few types of plants available for urban greening, and the cost of greening is relatively high. Especially in Heilongjiang Province, there are very few species that ...

AI Technical Summary

Problems solved by technology

[0004] The object of the present invention is to provide a kind of cold-resistant rose tissue culture breeding method, to solve the problem of low seed reproduction rate, large variation, slow propagation speed of cuttings and ramets, difficulty in inducing adventitious buds in the early stage of tissue culture, browning, etc., which cannot meet the needs of industrialization The problem of seedling needs

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