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Preparation method of label-free photoelectrochemical sensor for zearalenone

A zearalenone and photoelectrochemical technology, applied in the direction of material electrochemical variables, can solve the problems of low photoelectric conversion efficiency and large band gap, and achieve the effect of improving sensitivity and enhancing transmission speed

Inactive Publication Date: 2018-03-02
FUJIAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, TiO 2 The band gap is large and can only be excited by ultraviolet light, so the photoelectric conversion efficiency is low in the visible light region

Method used

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  • Preparation method of label-free photoelectrochemical sensor for zearalenone
  • Preparation method of label-free photoelectrochemical sensor for zearalenone
  • Preparation method of label-free photoelectrochemical sensor for zearalenone

Examples

Experimental program
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Effect test

Embodiment 1

[0028] A TiO-based 2 The preparation method of the label-free photoelectrochemical sensor of mesoscopic crystal (such as figure 1 shown):

[0029] (1) Pretreatment of the glassy carbon electrode: the glassy carbon electrode is first mechanically polished on the suede covered with alumina powder, washed with secondary water to remove the residual powder on the surface, and then moved into an ultrasonic water bath for cleaning until it is cleaned. Wash thoroughly with ethanol, dilute acid and water;

[0030] (2) Add dropwise 4 μL of RTM suspension with a concentration of 3 mg / mL on the surface of a clean glassy carbon electrode, dry under infrared light, and cool to room temperature;

[0031] (3) Then drop-coat 4 μL of 1 mg / mL Cu-Co-Fe solution onto the surface of the modified electrode, dry it under an infrared lamp, and then cool it at room temperature;

[0032] (4) Then, drop-coat 4 μL of 3 mg / mL polylysine solution onto the surface of the modified electrode, dry and cool ...

Embodiment 2

[0035] A TiO-based 2 The photoelectrochemical sensor of mesoscopic crystal is used for the detection method of zearalenone, and the steps are as follows:

[0036] (1) The three-electrode system was used for measurement, the PL / Cu-Co-Fe / RTM / Ab / ZEN modified electrode prepared in Example 1 was used as the working electrode, Ag / AgCl was used as the reference electrode, and the platinum wire electrode was used as the counter electrode , use the photoelectrochemical workstation to detect, set the voltage to 0.1V, switch the lamp every 10s, and the monochromatic light emitted by the xenon lamp to excite the light source is filtered by the monochromator before use;

[0037] (2) In PBS buffer solution with pH 6.5, detect 1×10 by photoelectrochemical workstation -6 ng / mL-10ng / mL series of zearalenone standard solutions with different concentrations, by recording the different current signals before and after switching the lamp, draw the working curve. Figure 2 A is different concentr...

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Abstract

The invention discloses a preparation method of a label-free photoelectrochemical sensor for zearalenone. According to a construction method of a sensing interface, a nano copper-cobalt-iron material,polylysine and nano-rutile type TiO2 mesoscopic crystals are taken as construction elements, and a zearalenone antibody (Ab) is further immobilized; due to the surface plasma effect of the nano copper-cobalt-iron material and the excellent conductivity of the polylysine, the sensing interface can accelerate the photo-induced electron transfer rate of the nano-rutile type TiO2 mesoscopic crystalsand improve photocurrent signals of the nano-rutile type TiO2 mesoscopic crystals; furthermore, the polylysine is rich in amino, thus being conducive to the load of the antibody; when the zearalenoneand the immobilized zearalenone antibody generate an immunoreaction, the photoelectric signals at the sensing interface are significantly weakened due to a steric hindrance effect. A label-free photoelectric sensing method established based on the phenomenon can realize high sensitive detection of the zearalenone having a concentration within a range of 1*10<-6>-10ng / mL.

Description

technical field [0001] The invention belongs to the technical field of novel functional materials and biosensing detection, and in particular relates to a label-free photoelectrochemical sensor for the detection of zearalenone. Background technique [0002] Zearalenone (Zearalenone), also known as F-2 toxin, is a metabolite of Gibberella zeae. It is widely distributed in contaminated grains, agricultural by-products, and dairy products, especially corn and its processed products. Zearalenone has reproductive and developmental toxicity, immunotoxicity and strong teratogenic toxicity, etc. It can also affect endocrine and may induce tumors. At present, the bioassay methods for the detection of zearalenone mainly include enzyme-linked immunosorbent assay, electrochemistry, and fluorescence spectroscopy. However, the further enhancement of the sensitivity of these methods and the exploration of a sensing platform with low background signal, good reproducibility, and wide respo...

Claims

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Application Information

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IPC IPC(8): G01N27/26
CPCG01N27/26
Inventor 戴宏陈四红高利红林燕语
Owner FUJIAN NORMAL UNIV
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