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Method for preparing bone-derived alkaline phosphatase detection kit

A detection kit and phosphatase technology, applied in the field of medical testing, can solve problems such as insufficient sensitivity, and achieve high detection sensitivity, simple operation, and good stability

Inactive Publication Date: 2018-02-09
ANHUI IPROCOM BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But because the clinical critical value of bone-derived alkaline phosphatase is very low, when the content of bone-derived alkaline phosphatase in the sample is in the normal physiological range, that is, when the antigen concentration is very low, the amount of antigen-antibody polymer produced is small, The change in turbidity of the reaction solution is very small, and the change in absorbance cannot be detected, which means that the sensitivity of this method is not enough

Method used

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  • Method for preparing bone-derived alkaline phosphatase detection kit
  • Method for preparing bone-derived alkaline phosphatase detection kit
  • Method for preparing bone-derived alkaline phosphatase detection kit

Examples

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Comparison scheme
Effect test

Embodiment 1

[0043] The preparation method of a kind of bone-derived alkaline phosphatase detection kit of the present embodiment comprises the following steps:

[0044] (1) Prepare reagent R1 according to the following component contents:

[0045] Reagent R1:

[0046]

[0047] ① Dissolve disodium hydrogen phosphate, sodium dihydrogen phosphate, potassium chloride, and sodium chloride in purified water according to the component content of the above reagent R1, stir evenly, and prepare R1 buffer after fully dissolved;

[0048] ② Dissolve polyethylene glycol 6000, sodium azide, and Tween-20 in the R1 buffer according to the component content of the above reagent R1, stir evenly, and wait until all the raw materials are fully dissolved to prepare the reagent R1;

[0049] (2) Prepare reagent R2 according to the following component contents:

[0050] Reagent R2:

[0051]

[0052] ① Dissolve disodium hydrogen phosphate, sodium dihydrogen phosphate, potassium chloride, and sodium chlori...

Embodiment 2

[0060] The preparation method of a kind of bone-derived alkaline phosphatase detection kit of the present embodiment comprises the following steps:

[0061] (1) Prepare reagent R1 according to the following component contents:

[0062] Reagent R1:

[0063]

[0064] ① Dissolve disodium hydrogen phosphate, sodium dihydrogen phosphate, potassium chloride, and sodium chloride in purified water according to the component content of the above reagent R1, stir evenly, and prepare R1 buffer after fully dissolved;

[0065] ② Dissolve polyethylene glycol 6000, sodium azide, and Tween-20 in the R1 buffer according to the component content of the above reagent R1, stir evenly, and wait until all the raw materials are fully dissolved to prepare the reagent R1;

[0066] (2) Prepare reagent R2 according to the following component contents:

[0067] Reagent R2:

[0068]

[0069]

[0070] ① Dissolve disodium hydrogen phosphate, sodium dihydrogen phosphate, potassium chloride, and s...

Embodiment 3

[0078] The preparation method of a kind of bone-derived alkaline phosphatase detection kit of the present embodiment comprises the following steps:

[0079] (1) Prepare reagent R1 according to the following component contents:

[0080] Reagent R1:

[0081]

[0082] ① Dissolve disodium hydrogen phosphate, sodium dihydrogen phosphate, potassium chloride, and sodium chloride in purified water according to the component content of the above reagent R1, stir evenly, and prepare R1 buffer after fully dissolved;

[0083] ② Dissolve polyethylene glycol 6000, sodium azide, and Tween-20 in the R1 buffer according to the component content of the above reagent R1, stir evenly, and wait until all the raw materials are fully dissolved to prepare the reagent R1;

[0084] (2) Prepare reagent R2 according to the following component contents:

[0085] Reagent R2:

[0086]

[0087]

[0088] ① Dissolve disodium hydrogen phosphate, sodium dihydrogen phosphate, potassium chloride, and s...

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Abstract

The invention discloses a method for preparing a bone-derived alkaline phosphatase detection kit. The method includes a step (1) of dissolving sodium hydrogen phosphate, sodium dihydrogen phosphate, potassium chloride and sodium chloride in purified water according to the component content of a reagent R1, to prepare an R1 buffer solution; dissolving polyethylene glycol 6000, sodium azide and Tween-20 in the R1 buffer solution, to obtain a reagent R1; a step (2) of dissolving sodium hydrogen phosphate, sodium dihydrogen phosphate, potassium chloride and sodium chloride in purified water according to the component content of a reagent R2, to prepare an R2 buffer solution; dissolving the Tween-20, polyoxyethylene-polystyrene copolymer and sodium azide in the R2 buffer solution, to obtain a dispersion liquid; preparing bone-derived alkaline phosphatase specific antibody latex particles; using the dispersion liquid to dissolve the bone-derived alkaline phosphatase specific antibody latex particles, and performing ultrasonic dispersion to obtain a reagent R2. The method has the advantages that the prepared kit has higher sensitivity and stability and the operation is simple and rapid.

Description

technical field [0001] The invention relates to the technical field of medical testing, in particular to a preparation method of a bone-derived alkaline phosphatase detection kit. Background technique [0002] Bone-derived alkaline phosphatase is secreted from the bone. When the calcium salt precipitation in the bone is insufficient, the secretion of the enzyme increases, and when the calcium salt in the bone is sufficient, the secretion decreases; therefore, the bone-derived alkaline phosphatase can be used for Check the body for insufficient calcium absorption. [0003] Bone alkaline phosphatase (NBAP) is one of the phenotypic markers of osteoblasts. It can directly reflect the activity or function of osteoblasts. The reference index is also the best index currently used to evaluate human bone mineralization disorders. To detect the catalytic activity of bone-derived alkaline phosphatase in children's blood, and to screen or assist in the diagnosis of bone calcification ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N35/00G01N33/52G01N1/38
CPCG01N35/00G01N1/38G01N33/52
Inventor 吴泽东庄庆华张金东吴铮朱雨周珍珍
Owner ANHUI IPROCOM BIOTECH CO LTD
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