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Preparation method and applications of polypyrrole functional nanoparticles stabilized with protein

A technology of functional nanoparticles and polypyrrole, which is applied in the fields of materials science and biomedicine, can solve the problems of limiting the clinical application of multifunctional photothermal agents, PPy is chemically inert, and lacks reactive sites, etc., and achieves excellent MRI effects, excellent Effect of photothermal performance, high clinical research value

Active Publication Date: 2018-02-09
HUBEI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the strong chemical inertness of PPy and the lack of reactive sites, the construction of PPy-based integrated diagnostic and therapeutic nanoparticles usually requires cumbersome reaction steps and harsh reaction conditions, which largely limit the relevant multifunctional photothermal The clinical application of the agent

Method used

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  • Preparation method and applications of polypyrrole functional nanoparticles stabilized with protein
  • Preparation method and applications of polypyrrole functional nanoparticles stabilized with protein
  • Preparation method and applications of polypyrrole functional nanoparticles stabilized with protein

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] A method for preparing protein-stabilized polypyrrole functional nanoparticles, specifically achieved through the following technical solutions:

[0029] Step 1: Weigh 45 mg of bovine serum albumin powder and dissolve it in 10 mL of deionized water, add 34 mg of pyrrole monomer, and stir thoroughly to obtain a uniform and transparent solution; then weigh 56 mg of recrystallized KPS and dissolve it in 6 mL of deionized water, then slowly Dropping the above transparent solution and keeping it warm at 4°C for 18 hours is conducive to the full polymerization of pyrrole monomers. During this period, it can be found that the solution quickly turns dark green and then dark black, indicating the successful formation of polypyrrole.

[0030] Step 2: Add 350 μL of gadolinium chloride solution with a concentration of 0.025M to the black solution in step 1, mix thoroughly for 22 minutes, then adjust the pH of the system to 11 with a 2M sodium hydroxide solution, and place in a water...

Embodiment 2

[0033] A method for preparing protein-stabilized polypyrrole functional nanoparticles, specifically achieved through the following technical solutions:

[0034] Step 1: Weigh 30 mg of bovine serum albumin powder and dissolve it in 10 mL of deionized water, add 15 mg of pyrrole monomer, stir well to obtain a uniform and transparent solution; then weigh 15 mg of recrystallized KPS and dissolve it in 2.5 mL of deionized water, then slowly Dropping the above transparent solution and keeping it warm at 4°C for 12 hours is conducive to the full polymerization of pyrrole monomers. During this period, it can be found that the solution quickly turns dark green and then dark black, indicating the successful formation of polypyrrole.

[0035] Step 2: Add 300 μL of 0.025M gadolinium chloride solution to the black solution in step 1, mix thoroughly for 15 minutes, then adjust the pH of the system to 10 with a 2M sodium hydroxide solution, and place in a water bath at 37°C Incubate for 6 ho...

Embodiment 3

[0037] A method for preparing protein-stabilized polypyrrole functional nanoparticles, specifically achieved through the following technical solutions:

[0038] Step 1: Weigh 60 mg of bovine serum albumin powder and dissolve it in 15 mL of deionized water, add pyrrole monomer of the same mass as bovine serum albumin powder, and stir well to obtain a uniform and transparent solution; then weigh 90 mg of recrystallized KPS and dissolve it in 15 mL Then slowly drop into the above transparent solution in deionized water, and keep it warm at 4°C for 12 hours, which is conducive to the full polymerization of pyrrole monomers. During this period, it can be found that the solution quickly turns dark green and then dark black, indicating the successful formation of polypyrrole. .

[0039] Step 2: Add 400 μL of 0.025M gadolinium chloride solution to the black solution in step 1, mix thoroughly for 30 minutes, then adjust the pH of the system to 12 with a 2M ammonia solution, and keep wa...

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Abstract

The invention discloses a preparation method and applications of polypyrrole functional nanoparticles stabilized with a protein, and belongs to the field of material science and biomedical application. According to the preparation method, water soluble bovine serum albumin (BSA) is taken as a stabilizing agent, potassium peroxydisulfate (KPS) is taken as an initiator, initiation of pyrrole monomerpolymerization is carried out, gadolinium ions (Gd3+) are introduced into a polypyrrole nanoparticle dispersion liquid stabilized by the protein, simulative biomineralization is adopted so as to realize in-suit generation of gadolinium oxide (Gd2O3) in polypyrrole nanoparticles stabilized by the protein. Synchronous diagnosis and treatment is realized, the polypyrrole functional nanoparticles stabilized with the protein possess excellent Ti modal MRI effect and light-heat properties, the preparation method is simple, reaction conditions are mild, the repeatability is high, and popularizationis convenient.

Description

technical field [0001] The invention belongs to the field of material science and biomedicine, and in particular relates to a preparation method and application of functional nanoparticles integrating nuclear magnetic resonance imaging (MRI) and photothermal therapy (PTT). Background technique [0002] In recent years, photothermal therapy has become a research hotspot in the field of tumor treatment due to its advantages of less trauma and less toxic side effects. PTT is the use of related photothermal therapeutic agents to absorb near-infrared light with low energy and strong tissue permeability and convert it into sufficient heat. The local high temperature (>43°C) in the tumor area directly leads to tumor cell apoptosis and tumor growth. A treatment method for tissue necrosis, in which the performance of photothermal agents plays a decisive role in the therapeutic effect. Polypyrrole (PPy) stands out in the research of photothermal agents for its excellent phototherma...

Claims

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Application Information

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IPC IPC(8): C08L89/00C08L79/04C08K3/22C08J3/07C08G73/06A61K49/18A61K49/12A61K49/08A61K41/00
CPCA61K41/0052A61K49/08A61K49/12A61K49/1824C08G73/0611C08J3/07C08J2389/00C08J2479/04C08K2003/221C08L89/00C08L79/04C08K3/22
Inventor 徐祖顺阳哲郑子威
Owner HUBEI UNIV
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