Kit for quantitatively determining NGAL (neutrophil gelatinase-associated lipocalin) in urine or blood

Abstract

The invention relates to a kit for quantitatively determining the NGAL (neutrophil gelatinase-associated lipocalin) in urine or blood. The kit comprises a kit cover, a kit body, a first reagent unit and a second reagent unit, wherein the first reagent unit and the second reagent unit are arranged in the kit body; the outside of the first reagent unit and the outside of the second reagent unit aremutually independent; the first reagent unit and the second reagent unit are connected through a support frame; fixing articles are filled around the two reagent units connected through the support frame; reagents contained by the first reagent unit include phosphate buffer solution, triton, polyethylene glycol and sodium azide; reagents contained by the second reagent unit include mouse anti-human NGAL monoclonal antibody modified latex particles, goat anti-human NGAL polyclonal antibody modified latex particles, casein and sodium azide. The kit has the advantages that the use and the operation are simple and convenient; the NGAL in the urine or blood can be fast, accurately and quantitatively determined; the detection sensitivity is improved; the linear measurement range is increased; the clinical detection requirements are fully met.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to a kit for quantitatively detecting NGAL in urine or blood. The kit is a testing kit suitable for use in a fully automatic biochemical analyzer and can be used for clinical auxiliary diagnosis. Background technique [0002] Up to 50% of patients in the intensive care unit may have some degree of acute renal failure. The mortality rate of acute renal failure is at the forefront of the mortality rate of critical illness. Prediction of acute renal failure has not improved significantly over the past 40 years. At present, the most commonly used biological indicator for clinical judgment of human kidney injury is the concentration of serum creatinine, but the concentration of serum creatinine can only be detected after a certain degree of kidney injury, and serum creatinine is not an ideal indicator for early diagnosis. Although the relatively new biological index serum cystatin C that has...

AI Technical Summary

Problems solved by technology

In addition, the existing kits generally have two reagent units and the box body as an integrated structure. The integrated structure has good stability, but it cannot freely replace the reagent units (reagent bottles), which makes the kit consume a lot of money and cost.

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