Endophytic bacterium with antagonistic effect on plant pathogenic fungi

A technology of bacilli and strains, applied in the directions of plant growth regulators, bacteria, botanical equipment and methods, etc., to achieve the effect of safe biocontrol and stable biocontrol

Active Publication Date: 2018-01-26
广州市林业和园林科学研究院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there is no report on the application of endophytic bacteria to control fungal diseases such as tree brown root disease, and the corresponding selection of bacteria and their antagonistic bacteria is also an important factor and index for the selection of endophytic bacteria for biocontrol

Method used

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  • Endophytic bacterium with antagonistic effect on plant pathogenic fungi
  • Endophytic bacterium with antagonistic effect on plant pathogenic fungi
  • Endophytic bacterium with antagonistic effect on plant pathogenic fungi

Examples

Experimental program
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Effect test

Embodiment 1

[0036] Example 1 Isolation and identification of Bacillus amyloliquefaciens strain BSY3

[0037] 1. Isolate strains from marigold plants collected in Guangzhou Baiyun Nursery. The specific isolation method is as follows:

[0038] (1) Single colony isolation

[0039] 1) Clean the collected fresh marigold leaf material with tap water;

[0040] 2) After rinsing once with sterile water, soak in 75% alcohol for 5 minutes, soak in 2% sodium hypochlorite solution for 2 minutes, and then rinse with sterile water 4 times, and spread the sterile water after the last rinse as a blank control ;

[0041] 3) Put the sterilized fresh plant material in a sterilized mortar, add a small amount of sterile water to grind it into a homogenate, and then dilute it into different gradients of the grinding solution and spread it on a flat plate;

[0042] 4) Place the plate in a 30°C incubator for culture, observe the growth of the colonies, then pick colonies of different shapes, purify and cultivate...

Embodiment 2

[0062] Example 2 Optimum culture condition optimization of Bacillus amyloliquefaciens strain BSY3

[0063] 1. The most suitable medium for strain growth

[0064] (1) Optimization method: Strain BSY3 was streaked and activated on LB medium (30°C), then inoculated into LB liquid medium, and activated at 280 rpm and 30°C. The activated strain BSY3 was inoculated into the shake flask fermentation basal medium YGN [10 g of yeast extract (with different nitrogen sources), 10 g of glucose (with different carbon sources), 5 g of NaCl (with different inorganic salts) at a 0.5 % inoculation amount. time), dilute to 1 L, pH 7.0]. After inoculation, culture in a shaker at 30 °C and 160 rpm for 2 to 5 days, and then centrifuge the fermentation broth of the above-mentioned different substances (carbon source, nitrogen source and inorganic salt) at 12000 rpm for 15 min, and the supernatant was filtered through 0.22 μm The original fermentation liquid is obtained after filtration by the por...

Embodiment 3

[0078]Example 3 Detection of the antagonistic effect of Bacillus amyloliquefaciens strain BSY3 on common pathogenic fungi

[0079] 1. The BSY3 strain was co-cultured with several pathogenic fungi, and its antagonism to pathogenic fungi was detected.

[0080] Tested strains: Harmful Phylonotus xylinum ( P. noxius ), white butterfly anthracnose pathogen ( Colletotrichum sp.), canna blast pathogen ( Pyricularia cannaecola ), black pine leaf spot pathogen ( Pestalotiopsis sp.), the pathogen of banana wilt ( Fusarium oxysporum f. sp. cubes ) and Sclerotinia sclerotiorum pathogen ( Sclerotinia sclerotiorum ).

[0081] 2. Persistence culture detection method:

[0082] (1) Inoculate a 1% (v / v) ratio of the bacterial solution into a 150 mL Erlenmeyer flask filled with 40 mL LB medium, and incubate at 28°C and 200 rpm for 2.5 days;

[0083] (2) Centrifuge at 8000 rpm for 10 min to collect the supernatant, and then pass through a 0.22 μm filter membrane to obtain the crud...

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Abstract

The invention discloses an endophytic bacterium with an antagonistic effect on plant pathogenic fungi. Particularly, the endophytic bacterium is a bacillus amyloliquefaciens strain BSY3, is preservedat the CCTCC (China Center for Type Culture Collection) on August 31, 2017 with the preservation number being CCTCC NO: M 2017463. The strain BSY3 has the obvious antagonistic effect on common plant fungi such as phellinus noxius, pyricularia cannaecola hashioka, sclerotinia sclerotiorum, colletotrichum sp., pestalotiopsis sp., fusarium oxysporumf.sp.cubense and the like, and has a remarkable biocontrol function on fungi diseases caused by the pathogens. Besides, the strain is the endophytic bacterium derived from plants, and has a safer biocontrol effect on fungal diseases compared with a traditional chemical agent control method; the endophytic bacterium can establish a survival relationship of harmonious coexistence and stable development with the plants, and the biocontrol function ofthe endophytic bacterium can be played more effectively and stably.

Description

technical field [0001] The invention belongs to the technical field of plant disease biological control. More specifically, it relates to an endophytic bacterium that is antagonistic to phytopathogenic fungi. Background technique [0002] Tree fungal diseases are forest diseases caused by pathogenic fungi, such as brown root disease, which are caused by harmful Phylopores ( Phellinus noxius ) is a soil-borne disease that causes devastating damage to commercial crops, fruit trees, ancient and famous trees, and landscaping plants. It is known as "tree cancer". At present, prevention and control measures such as cutting down diseased trees, digging ditches to block the spread of pathogens, fumigation of diseased soil, and planting disease-free seedlings are mainly taken, but there is no ideal control agent and control method that can effectively control the occurrence of tree brown root disease. . In addition, although there are currently some chemical agents that can effect...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/02A01P3/00C12R1/07
Inventor 孙龙华黄华枝阮琳毕可可邓嘉茹张劲蔼吴超唐立鸿胡彦辉邓从双候亚会
Owner 广州市林业和园林科学研究院
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