Heat-resisting type bifidobacterium longum, screening method thereof and preparation method for bacterial powder

Abstract

The invention relates to the field of probiotics and especially relates to a heat-resisting type bifidobacterium longum, a screening method thereof and a preparation method for bacterial powder. The heat-resisting type bifidobacterium longum is preserved at China General Microbiological Culture Collection Center with the preservation number of CGMCC NO.10452. The screening method thereof comprisesthe steps of collecting of samples, conditional preliminary screening and secondarily screening for stress resistance. The preparation method thereof comprises the following steps: activating bacterial strain, expanding culture, anaerobically fermenting, collecting and emulsifying thalli, freeze-drying emulsion, crushing and then packaging. The heat-resisting type bifidobacterium longum providedby the invention has high activity, is capable of obviously promoting the stability within the shelf life under normal temperature or high temperature and has excellent acid resistance, cholate resistance and intestinal tract adhesion in human gastrointestinal tracts.

Description

technical field [0001] The invention relates to the field of probiotics, in particular to a heat-resistant bifidobacterium longum, a screening method thereof, and a preparation method of bacterial powder. Background technique [0002] Bifidobacterium longum belongs to probiotics that can be added to food, and mainly involves the application of food, health care products, daily chemical products, feed and other products. Probiotic powder, especially probiotic powder related to lactic acid bacteria, is common in most probiotic fermented foods, health product preparations, biological feed, and pharmaceutical preparations. [0003] Probiotics generally have high environmental requirements and poor tolerance to the outside world. It has been difficult to maintain high activity in long-term storage at room temperature, which limits product development and application. Although the probiotics currently on the market are supplied in the form of freeze-dried bacteria powder, the sto...

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Problems solved by technology

[0003] Probiotics generally have high requirements on the environment and poor tolerance to the outside world. It has been difficult to maintain high activity in long-term storage at room temperature, which limits product development and application.

Although the probiotics currently on the market are supplied in the form of freeze-dried bacteria powder, the storage stability of the probiotics generally shows a serious decline in the shelf life, especially for products stored at room temperature or at a high external temperature. Significant decline or sudden drop in the number of viable bacteria in a short period of time

[0004] At present, most domestic products of microecological preparations are stored at room temperature or at room temperature. In some areas or there are local periods of high temperature or long-term harsh weather, the storage conditions of the products cannot be guaranteed, or some products that require special processes need to go through high temperature for a long time. For example, in the manufacturing process of capsules, soft candies, chocolates and other products, the live bacteria in the products will be greatly lost, so a screening method that can improve the tolerance of the strain itself is needed to deal with the development and application of different products.

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