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Method for screening single bacterium for promoting dopamine components

A screening method and dopamine technology, applied in the field of microorganisms, can solve the problems of heavy screening work, long cycle, low screening accuracy, etc., and achieve the effects of excellent stability of viable bacterial count, short cycle, and high bacterial cell centrifugal collection rate.

Pending Publication Date: 2020-05-15
四川垚芯生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The screening methods in the prior art are mostly based on their biological and functional characteristics. These methods can be used for preliminary screening, but the screening work is heavy, the cycle is long, and the screening accuracy is low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 2

[0036] A screening method for a single bacterium that promotes dopamine composition of the present invention, the realization steps are as follows:

[0037] S1. Sample collection: dissolve the sample containing actinomycetes in sterile water, and treat it in a water bath at 65°C for 10 minutes to obtain an aqueous solution of mixed bacteria;

[0038] S2. Conditional preliminary screening: After the sample is uniformly mixed and diluted, transfer the liquid to the screening medium to coat and separate, and culture at an anaerobic constant temperature at 45°C for 72 hours; then select colonies with smooth edges and milky white bumps, and repeatedly detect them with a streaking microscope The bacteria are rod-shaped and bifurcated Gram-positive colonies. After repeated purification, continue anaerobic incubation at a constant temperature of 50°C for 36 hours;

[0039] S3. Re-screening for stress resistance: add the fungus slime to the diluent and mix evenly, then place it in a co...

Embodiment 3

[0047] A screening method for a single bacterium that promotes dopamine composition of the present invention, the realization steps are as follows:

[0048] S1. Sample collection: dissolve the sample containing actinomycetes in sterile water, and treat it in a water bath at 60°C for 8 minutes to obtain an aqueous solution of mixed bacteria;

[0049] S2. Conditional preliminary screening: After the sample is uniformly mixed and diluted, transfer the liquid to the screening medium to coat and separate, and incubate at an anaerobic constant temperature at 35°C for 50 hours; then select colonies with smooth edges and milky white bumps, and repeatedly detect them with a streaking microscope The bacteria are rod-shaped and bifurcated Gram-positive colonies. After repeated purification, continue anaerobic culture at 48°C for 32 hours;

[0050] S3. Re-screening for stress resistance: add the fungus slime to the diluent and mix evenly, then place it in a constant temperature water bath...

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Abstract

The invention discloses a method for screening a single bacterium for promoting dopamine components. The method disclosed by the invention comprises steps of sample collection, conditional primary screening and secondary screening for stress resistance. The preparation method comprises steps of strain activation and culture amplification, anaerobic fermentation and bacterium collection. The singlebacterium for promoting dopamine components has high activity, has remarkably improved stability within a normal temperature or high temperature shelf life, in addition, has good outdoor acid resistance and saline-alkaline tolerance, and has good adhesiveness to root systems of planted crops.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to a method for screening single bacteria that promote dopamine components. Background technique [0002] Actinomycetes are closely related to human production and life, and about 70% of widely used antibiotics are produced by various actinomycetes. Some types of actinomycetes can also produce various enzymes (protease, amylase, and cellulase, etc.), vitamins (B12) and organic acids. Actinomycetes are widely distributed in nature, and mainly exist in soil, air and water in the form of spores or mycelia, especially in soils with low water content, rich organic matter, neutral or slightly alkaline soils. Actinomycetes are only morphologically classified and belong to the phylum Actinomycetes of the Bacteria Kingdom. The peculiar muddy smell of the soil is mainly caused by the metabolites of actinomycetes. [0003] Dopamine (C6H3(OH)2-CH2-CH2-NH2) is secreted by the brain and can affec...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/04
CPCC12Q1/04
Inventor 赵西韩
Owner 四川垚芯生物科技有限公司
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