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Preparation method of biological soil solidifier

A biological soil and curing agent technology, applied in the field of biological soil curing agent preparation, can solve the problems of poor soil porosity and permeability, low compressive strength, etc., and achieve the effect of improving soil strength and hardness and promoting sedimentation

Inactive Publication Date: 2018-01-19
朱东洋
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] 6. The technical problem to be solved by the present invention: Aiming at the low compressive strength of the current soil curing agent and the poor effect on soil porosity and permeability, a preparation method of biological soil curing agent is proposed

Method used

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  • Preparation method of biological soil solidifier

Examples

Experimental program
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Effect test

example 1

[0036] Bacteria: the freeze-dried powder of Bacillus pasteurianus and Bacillus subtilis used in the present invention.

[0037] Preparation of solid medium: by mass parts, get 6 parts of glucose, 15 parts of tryptone, 5 parts of yeast extract, (NH4) 2 HPO 4 0.2 parts, KH 2 PO 4 0.1 parts, MgSO 4 •7H 2 0.04 parts of O, 12 parts of NaCl, 10 parts of agar, 0.05 parts of phosphate buffer, 1000 parts of water, sterilized at 121°C for 20 minutes, pH 5.4.

[0038] Preparation of liquid medium: remove the agar components and keep the other components unchanged.

[0039] Preparation of catalytic enzyme fermentation medium: by mass parts, 0.5 parts of peptone, 10 parts of glucose, KH 2 PO 4 0.05 parts, Na 2 HPO 4 2 parts MgSO 4 •7H 2 0.02 parts of O, 5 parts of urea, pH 6.8, urea was sterilized under ultraviolet light for 30 minutes and then mixed.

[0040] Quartz sand pretreatment: use standard quartz sand (containing 99.7% quartz), uniform gradation (gradation coefficien...

example 2

[0047] Bacteria: the freeze-dried powder of Bacillus pasteurianus and Bacillus subtilis used in the present invention.

[0048] Preparation of solid medium: by mass parts, get 8 parts of glucose, 20 parts of tryptone, 10 parts of yeast extract, (NH4) 2 HPO 4 0.4 parts, KH 2 PO 4 0.4 parts, MgSO 4 •7H 2 0.1 part of O, 15 parts of NaCl, 20 parts of agar, 0.1 part of phosphate buffer, 1000 parts of water, sterilized at 121°C for 20 minutes, pH 5.8.

[0049] Preparation of liquid medium: remove the agar components and keep the other components unchanged.

[0050] Preparation of catalytic enzyme fermentation medium: by mass parts, 0.8 parts of peptone, 12 parts of glucose, KH 2 PO 4 0.1 parts, Na 2 HPO 4 5 parts, MgSO 4 •7H 2 0.05 parts of O, 10 parts of urea, pH 7.2, urea was sterilized under ultraviolet light for 30 minutes and then mixed.

[0051] Quartz sand pretreatment: use standard quartz sand (containing 99.7% quartz), uniform gradation (gradation coefficient ...

example 3

[0058] Bacteria: the freeze-dried powder of Bacillus pasteurianus and Bacillus subtilis used in the present invention.

[0059] Preparation of solid medium: by mass parts, get 7 parts of glucose, 17.5 parts of tryptone, 7.5 parts of yeast extract, (NH4) 2 HPO 4 0.3 parts, KH 2 PO 4 0.25 parts, MgSO 4 •7H 2 0.07 parts of O, 13.5 parts of NaCl, 15 parts of agar, 0.75 parts of phosphate buffer, 1000 parts of water, sterilized at 121°C for 20 minutes, pH 5.6.

[0060] Preparation of liquid medium: remove the agar components and keep the other components unchanged.

[0061] Preparation of catalytic enzyme fermentation medium: by mass parts, 0.65 parts of peptone, 11 parts of glucose, KH 2 PO 4 0.07 parts, Na 2 HPO 4 3.5 parts, MgSO 4 •7H 2 0.35 parts of O, 7.5 parts of urea, pH7, urea was sterilized under ultraviolet light for 30 minutes and then mixed.

[0062] Quartz sand pretreatment: use standard quartz sand (containing 99.7% quartz), uniform gradation (gradation ...

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Abstract

The invention discloses a preparation method of a biological soil solidifier and belongs to the field of soil solidifiers. According to the preparation method disclosed by the invention, a high-activity catalyzing enzyme is extracted from strains; the enzyme can be used for efficiently catalyzing urea to be hydrolyzed into ammonia and carbon dioxide and is dispersed into a solution through cell walls; calcium ions in the solution can be adsorbed on surfaces of bacterium cells; after the urea is hydrolyzed, generated carbonate radicals are precipitated with the calcium ions to generate calciumcarbonate sediment for covering bacteria; ammonium ions generated by decomposing the urea in a culture solution are good for the generation of the calcium carbonate sediment; generated calcium carbonate crystals are filled into soil grains to adhere the adjacent soil grains; except a calcium carbonate crystal with a cohering effect, part of soil grains cover the surface of the sandy soil grains orare freely distributed in a sample, so that soil properties are also reduced (the soil porosity and the permeability are reduced). By adopting the preparation method of the biological soil solidifier, the problems of a current soil solidifier that the compressive strength is reduced and the soil porosity and the permeability effect are poor are solved.

Description

technical field [0001] The invention belongs to the field of soil curing agents, and in particular relates to a preparation method of biological soil curing agents. Background technique [0002] Soil curing agent refers to adding to the soil at room temperature, which can directly cement the surface of soil particles in the soil or can undergo hydration reaction, ion combination, biological action and other processes with clay minerals, and can improve soil compactness and strength to a certain extent. , elasticity and water resistance properties. For the soil to be reinforced, according to the physical and chemical properties of the soil, it only needs to be mixed with a certain amount of curing agent, and the required performance index can be achieved after mixing and compacting. In the 1940s, foreign countries first applied soil stabilizers in road engineering to obtain good results and developed rapidly. In the 1990s, soil stabilizer technology entered China and has...

Claims

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Application Information

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IPC IPC(8): C09K17/40C12N1/20C12N9/00C12R1/125C12R1/07C09K103/00
Inventor 朱东洋李璐刘沁
Owner 朱东洋
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