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Method for extracting juglanin from apple flowers

A technology of apple blossom and juglantin, which is applied in chemical instruments and methods, preparation of sugar derivatives, sugar derivatives, etc., to achieve good selectivity, stable chemical properties, and easy elution and regeneration

Active Publication Date: 2018-01-19
周静宜
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there is no report about the extraction and isolation of juglanin from apple flowers

Method used

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  • Method for extracting juglanin from apple flowers

Examples

Experimental program
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Effect test

Embodiment 1

[0023] A method for extracting jugranin from apple flowers, its steps are as follows:

[0024] (1) Take the dried apple blossoms, crush them, and degreas them twice by cold soaking in petroleum ether at room temperature, each time is 2 d, the mass ratio of the dried apple blossoms to the volume of petroleum ether is 1:10, g / mL, residue Volatilize the petroleum ether, add ethanol, the volume fraction of ethanol is 60%, the mass ratio of the residue to the ethanol is 1:8, g / mL, cold immersion extraction twice, 1.5 d each time, the ethanol is recovered under reduced pressure. Obtain the total ethanol extract;

[0025] (2) Disperse the total ethanol extract in water. The mass ratio of the total ethanol extract to water is 1:5. Extract with petroleum ether. The volume ratio of petroleum ether to water is 1:1. After static stratification, remove the lower layer. The ethanol-water solution after petroleum ether extraction is extracted with ethyl acetate. The volume ratio of the ethanol-w...

Embodiment 2

[0028] A method for extracting jugranin from apple flowers, its steps are as follows:

[0029] (1) Take the dried apple blossoms, pulverize, and defatted by cold soaking in petroleum ether at room temperature for 3 times, each time is 3 days, the mass of the dried apple blossoms and the volume ratio of petroleum ether are 1:12, g / mL, residue Volatilize the petroleum ether, add ethanol, the volume fraction of ethanol is 85%, the mass of the residue and the volume ratio of ethanol is 1:14, g / mL, cold soak extraction 3 times, 3 days each time, decompression recovery ethanol, get 70% ethanol total extract;

[0030] (2) Disperse the total ethanol extract in water. The mass ratio of total ethanol extract to water is 1:10. Extract with petroleum ether. The volume ratio of petroleum ether to water is 1:3. After static layering, take the upper layer Petroleum ether layer, the solvent is recovered under reduced pressure to obtain the petroleum ether extract, and then the ethanol-water solut...

Embodiment 3

[0033] A method for extracting jugranin from apple flowers, its steps are as follows:

[0034] (1) Take the dried apple blossoms, pulverize, and defatted by cold soaking in petroleum ether at room temperature for 3 times, each time is 3 days, the mass of the dried apple blossoms and the volume ratio of petroleum ether are 1:12, g / mL, residue Volatilize the petroleum ether and add ethanol. The volume fraction of ethanol is 60-85%, and the mass ratio of residue to ethanol is 1:10, g / mL. Cold immersion extraction is performed twice, 2 days each time, and ethanol is recovered under reduced pressure. , Get the total ethanol extract;

[0035] (2) Disperse the total ethanol extract in water, the mass ratio of the total ethanol extract to water is 1:8, extract with petroleum ether, the volume ratio of petroleum ether to water is 1:2, after static stratification, take the upper layer Petroleum ether layer, the solvent is recovered under reduced pressure to obtain the petroleum ether extrac...

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Abstract

The invention discloses a method for extracting juglanin from apple flowers. The method comprises the following steps of (1) obtaining ethanol total extracts of dried apple flowers; (2) performing extraction on the ethanol total extracts to obtain apple flower normal butanol positions; (3) performing normal pressure column chromatography separation on the apple flower normal butanol positions through silica gel; performing gradient elution and TLC thin layer chromatography tracking detection merging to obtain six ingredients; performing normal pressure silica gel column chromatography separation on the fifth ingredient; performing gradient elution; collecting two ingredients; performing Sephadex LH-20 gel column chromatography separation and recrystallization on the first ingredient to obtain a pure ingredient of juglanin. The used separation materials include the silica gel and sol column chromatography, and cannot be dissolved in water and any solvents; no toxicity and no smell exist; the chemical properties are stable; the selectivity on organic matters is better; the influence by the existence of inorganic salts and strong ion low molecular compounds is avoided; in addition, the elution regeneration is easy; the benefits to multi-time utilization can be realized.

Description

Technical field [0001] The invention belongs to the technical field of plant extraction and separation, and specifically relates to a method for efficiently and rapidly extracting jugranin from apple flowers. Background technique [0002] Rosaceae apple Malus Pumila Mill of dry flowers. The main components of apple blossoms are dihydrochalcones such as phloretin and phlorizin; other flavonoids such as quercetin, kaempferin, rutin and so on. It has the effects of curing diabetes, metabolic syndrome and neurological diseases, anti-oxidation, improving memory, beauty and whitening. The processed apple blossom tea has the effects of nourishing blood, improving eyesight, detoxifying, treating neuralgia, and removing acne. [0003] At present, there is no report on the extraction and separation of jugranin from apple flowers. Summary of the invention [0004] The purpose of the present invention is to overcome the defects of the prior art, provide a simple process flow for separating ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H17/07C07H1/08
Inventor 周静宜
Owner 周静宜
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