Construction method for applicability evaluation model of immune state of T lymphocyte of hepatic diseases of hepatitis B

Abstract

The invention provides a construction method for an applicability evaluation model of an immune state of T lymphocyte of hepatic diseases of hepatitis B. The method comprises the following steps of (1) detecting T cell activator factors and inhibitory / exhaustion molecules of peripheral blood of a patient through a flow cytometry in order to obtain levels of the T cell factors of each detection sample; (2) collecting population statistics, virology, liver function and liver hardness value data of each case and carrying out correlation analysis on clinical indexes and a T cell immunity evaluation index to find the clinical indexes having the close correlation; (3) utilizing the different clinical indexes to establish a plurality of scored ROC curve models, comparing areas under the ROC curve, flexibilities and specificities of different models and selecting the optimal ROC curve; and (4) obtaining different immuno-clinic score equations according to the optimal ROC curve in order to further optimize the optimal immuno-clinic score equation used for evaluating the overall immune state of the T cell.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to an applied assessment model of T lymphocyte immune status of hepatitis B liver disease and a construction method thereof. technical background [0002] T lymphocytes (T lymphocytes), referred to as T cells, are lymphatic stem cells derived from bone marrow. After differentiation and maturation in the thymus, they are distributed to immune organs and tissues throughout the body through lymph and blood circulation to perform immune functions. The biological functions include: directly killing target cells, assisting or inhibiting B cells to produce antibodies, responding to specific antigens and mitogens, and producing cytokines; therefore, T cells are the heroic cells formed in the body to resist disease infections and tumors fighter. The immune response produced by T cells is cellular immunity. There are two main types of effect of cellular immunity: specific binding to ta...

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Problems solved by technology

[0008] With the development of life omics, the technology related to the immune system has been continuously improved. Since the 1970s, the determination of immunoglobulin and complement can roughly and indirectly estimate the immune status of the body, and it has been gradually used by lymphocyte subsets, cytokines, etc. Representative detection methods include: 1) Biological methods based on DNA detection, such as PCR, Northern blotting, in situ hybridization and other techniques, which can detect a single immune cytokine and study its regulatory pathways , not suitable for discrimination of clinical complex cases; 2) biological activity detection methods, such as ELISA, ELISPOT, etc., these methods only detect single cells or factors secreted by a single immune cell, rather than the average cytokine level of a certain group of immune cells Similarly, it is not conducive to the discrimination of clinically complicated cases; 3) flow cytometry and liquid chip detection: the current relatively accurate detection technology can detect multiple cytokines, and clinicians can detect different cytokines according to different cytokine levels. Estimate the immune status of patients; however, it is highly subjective, quite expensive, cumbersome to operate, and cannot be widely used

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