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Peony pollen phaseolin polypeptide prepared by using fermentation method

A technology of protein polypeptide and fermentation method, applied in the direction of fermentation, peptide source, plant peptide, etc., can solve the problems of weak activity of functional polypeptide, low extraction rate of peony protein polypeptide, limited application prospects, etc., to improve physical and chemical Absorptive power, significant blood pressure lowering effect, remarkable effect

Inactive Publication Date: 2018-01-05
磐安县派普特生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above products have a delicate taste, moderate sweet and sour, no bitter smell, but the extraction rate of peony protein polypeptide is not high, and the activity of the extracted functional polypeptide is not strong, so the application prospect is limited

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] The peony pollen protein polypeptide prepared by the fermentation method has the effect of losing weight and has an excellent effect of lowering blood pressure.

[0022] The method for preparing peony pollen protein polypeptide by fermentation method comprises the following steps:

[0023] 1) Pollen wall breaking: freeze the peony pollen at -40~-50°C for 2~24 hours, mechanically crush and break the wall, and obtain the broken peony pollen;

[0024] 2) Essential oil extraction: Add absolute ethanol to the peony petal powder according to the ratio of material to liquid: 1g: 2~6 mL, and place it in a microwave extraction apparatus for extraction. The microwave power is 200~400W, and the microwave action time is 15~35s. The number of times is 2 to 6 times, filter the extract and let it stand, remove the water layer after the layers are stable, add anhydrous sodium sulfate and let stand overnight, filter the filtrate, and rotary steam to obtain the peony essential oil;

[0...

Embodiment 2

[0029] The peony pollen protein polypeptide prepared by the fermentation method has the effect of losing weight and has an excellent effect of lowering blood pressure.

[0030]The most preferred method for preparing peony pollen protein polypeptide by fermentation method comprises the following steps:

[0031] 1) Pollen wall breaking: freeze peony pollen at -45°C for 12 hours, mechanically crush and break the wall, and obtain broken peony pollen;

[0032] 2) Essential oil extraction: Add absolute ethanol to the peony petal powder according to the material-to-liquid ratio of 1g: 4mL, and extract in a microwave extraction apparatus with a microwave power of 300W, a microwave action time of 25s, and extraction times of 3 times. The extract was left to stand, and after the layers were stable, the water layer was removed, anhydrous sodium sulfate was added to stand overnight, the filtrate was filtered, and the peony essential oil was obtained by rotary steaming;

[0033] 3) Fermen...

Embodiment 3

[0037] The peony pollen protein polypeptide prepared by the fermentation method has the effect of losing weight and has an excellent effect of lowering blood pressure.

[0038] The method for preparing peony pollen protein polypeptide by fermentation method comprises the following steps:

[0039] 1) Pollen wall breaking: freeze the peony pollen at -50°C for 12 hours, mechanically crush and break the wall, and obtain the broken wall peony pollen;

[0040] 2) Essential oil extraction: Add absolute ethanol to the peony petal powder according to the ratio of material to liquid: 1g: 6mL, and extract in a microwave extraction apparatus with a microwave power of 400W, a microwave action time of 15s, and extraction times of 3 times. The extract was left to stand, and after the layers were stable, the water layer was removed, anhydrous sodium sulfate was added to stand overnight, the filtrate was filtered, and the peony essential oil was obtained by rotary steaming;

[0041] 3) Fermen...

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PUM

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Abstract

The invention discloses peony pollen phaseolin polypeptide prepared by using a fermentation method. The phaseolin polypeptide has the weight losing function and excellent blood pressure reduction function. A method for preparing the peony pollen phaseolin polypeptide by using the fermentation method comprises the steps of pollen wall-breaking, essential oil extraction, fermentation, degreasing andspray drying. Peony pollen is frozen and subjected to mechanical pulverization, then lactobacillus plantarum and saccharomyces cerevisiae are adopted for anaerobic fermentation, wall-breaking continues, and then bacillus subtilis is adopted for fermentation; neutral protease generated by fermentation is used for conducting enzymolysis on peony pollen protein, the peony pollen phaseolin polypeptide with active intensity is obtained, and then chitosan immobilized lipase is adopted for degreasing fermentation liquid.

Description

technical field [0001] The invention relates to the field of biologically active protein polypeptides, in particular to a peony pollen protein polypeptide prepared by a fermentation method. Background technique [0002] peony( Paeonia suffruticosa Andr. ) belongs to the genus Paeoniae, Ranunculaceae, deciduous shrubs with high ornamental value and medicinal value. For a long time, the development and utilization of peony deep-processing products have been seriously lagging behind, the added value of production is very low, and no industrial chain has been formed. Peony pollen is not only nutritious but also contains a variety of biologically active substances. The amino acid content is more than 25.0%, rich in 17 kinds of amino acids, rich in vitamin B and vitamin C, and also contains biologically active substances such as peony polysaccharides and peony flavonoids. The protein content is 39.3%, which is much higher than that of general pollen. It is called the protein co...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/415C12P21/02A61P3/04A61P9/12C12N11/10A23L33/18C11B9/02C12R1/25C12R1/865
Inventor 陈雨诗
Owner 磐安县派普特生物科技有限公司
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