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Immunosuppressive cell preparation as well as preparation method and application thereof

A cell preparation and immunosuppressive technology, which is applied in the field of biomedicine, can solve the problems of the small number of Treg cells and the long expansion and culture period, and achieve the effect of solving the disorder of the body's immune system, improving the blood environment, and ensuring cell viability

Inactive Publication Date: 2017-12-29
广东颜值科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Treg cells have therapeutic effects on a variety of immune disorders, and have achieved remarkable results in animal models such as graft-versus-host reaction and autoimmune diseases. The number of cells is small and the expansion and culture period is long, thus limiting its clinical application

Method used

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  • Immunosuppressive cell preparation as well as preparation method and application thereof
  • Immunosuppressive cell preparation as well as preparation method and application thereof
  • Immunosuppressive cell preparation as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] 1. Preparation of exosomes from umbilical cord mesenchymal stem cells

[0025] (1) Primary culture of umbilical cord stem cells

[0026] Step 1: Take the umbilical cord, perform aseptic cleaning in the ultra-clean bench, peel off the umbilical cord amniotic membrane and arteries and veins, take out Wharton's glue, and cut it into pieces with surgical scissors;

[0027] Step 2: Resuspend with complete medium (DMEM+10% FBS) and inoculate into 15cm dish, transfer to 37℃, containing 5% CO 2 cultivated in an incubator;

[0028] Step 3: Replenish the liquid after 5 days until the cells climb out, discard the tissue pieces, supplement with new complete medium and continue to culture, and the cells can be passaged when they form larger clones;

[0029] (2) Subculture and identification of umbilical cord stem cells

[0030] Step 4: Take the primary cells that can be passaged, discard the original culture medium in the dish, add 10mL PBS buffer solution to gently wash the cell...

Embodiment 2

[0052] 1. 3 different concentrations of immunosuppressive cell preparations according to the present invention

[0053] According to the preparation method of the immunosuppressive cell preparation described in Example 1, according to the concentration of each component described in Table 2, three kinds of immunosuppressive cell preparations with different concentrations were prepared, corresponding to cell preparation 1, cell preparation 2 and cell preparation respectively 3.

[0054] Table 2

[0055]

[0056]

[0057] 2. SLE model mice and normal mice

[0058] SLE model mice were EB virus membrane antigen BLLF1 gene transgenic mice, 42 females, weighing 30-40 g; 6 normal mice, weighing 30-40 g, purchased from the Experimental Animal Research Center of Sun Yat-sen University Medical School.

[0059] 3. Grouping and administration of animals

[0060] The normal mice were used as the blank group, and the SLE model mice were randomly divided into 7 groups, 6 in each gro...

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PUM

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Abstract

The invention belongs to the technical field of biological medicine and particularly relates to an immunosuppressive cell preparation as well as a preparation method and an application thereof. The invention provides the immunosuppressive cell preparation and the application of the immunosuppressive cell preparation in preparation of SLE (systemic lupus erythematosus) preventing and treating drugs and further provides the preparation method of the immunosuppressive cell preparation. The immunosuppressive cell preparation comprises Treg cells, umbilical cord mesenchymal stem cell exosomes, cord blood mononuclear cells, umbilical cord blood plasma and a medically acceptable solvent. The immunosuppressive cell preparation has the beneficial effects as follows: the Treg cells and the umbilical cord mesenchymal stem cell exosomes play a role in inhibiting body immunity hyperfunction and balancing body immune system function firstly, the cord blood mononuclear cells play a role in immunoregulation, blood environment is improved, and the problem of immune system disorder is solved fundamentally.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to an immunosuppressive cell preparation and its preparation method and application. Background technique [0002] Systemic Lupus Erythematosus (SLE) is a diffuse, systemic autoimmune disease. Under the interaction of various factors such as environmental factors and estrogen levels, T lymphocytes decrease, T suppressor cell function decreases, B cells overproliferate, and a large number of autoantibodies are produced, which combine with corresponding autoantigens in the body to form corresponding immune responses. Complex, deposited in the skin, joints, small blood vessels, glomeruli and other parts. With the participation of complement, acute and chronic inflammation and tissue necrosis are caused (such as lupus nephritis), or antibodies directly interact with tissue cell antigens, causing cell destruction (such as specific antigens of red blood cells, lymphocytes...

Claims

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Application Information

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IPC IPC(8): A61K35/51A61P37/02A61K35/17A61K35/28
Inventor 马颖郑皓钧
Owner 广东颜值科技有限公司
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