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Menstrual blood stem cell preparation for treating simple skin damage and preparation method thereof

A technology of stem cell preparation and skin damage, which is applied in the field of menstrual blood stem cell preparation and its preparation for the treatment of simple skin damage, which can solve the problems of obvious scar formation, unsatisfactory conditions, and unsatisfactory effects, etc., and achieve strong cell regeneration and repair , Excellent effect, good tissue repair effect

Active Publication Date: 2020-12-08
成都远山博桥生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The use of drugs in the prior art for tissue repair after simple skin damage has the problems of unsatisfactory repair and obvious scar formation
However, the effect of using menstrual blood stem cell culture fluid or cytokines alone for tissue repair is not satisfactory.

Method used

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  • Menstrual blood stem cell preparation for treating simple skin damage and preparation method thereof
  • Menstrual blood stem cell preparation for treating simple skin damage and preparation method thereof
  • Menstrual blood stem cell preparation for treating simple skin damage and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] A menstrual blood stem cell preparation for treating simple skin damage, comprising cell breaking solution, and rhEGF and KGF added to the cell breaking solution.

[0036] The preparation method of the above-mentioned cell disruption liquid is as follows:

[0037] Take 10 mL of menstrual blood, add 10 mL of human lymphocyte separation medium, centrifuge at 2500 rpm for 15-25 minutes, collect mononuclear cell layer and intima tissue, add 5 mL of serum-free medium for mesenchymal stem cells developed by CellGenix, and transfer to T25 culture Primary culture in flasks. The serum-free medium of the mesenchymal stem cells was added bFGF at a final concentration of 30 ng / mL, hLif at a final concentration of 30 ng / mL, and gentamicin at a final concentration of 400 IU / mL. The added bFGF, hLif and gentamycin all meet the GMP standard.

[0038] Change the medium after 8 hours of cultivation, and transfer the supernatant in the T25 culture bottle to continue the culture. If the ...

Embodiment 2

[0041] The difference between this example and Example 1 is that in this example there is only the cell disruption solution, and rhEGF and KGF are not added to the cell disruption solution.

Embodiment 3

[0043] This example is a comparative example of Example 1, and the preparation in this example only consists of rhEGF with a final concentration of 0.08 μg / mL and KGF with a final concentration of 0.4 μg / mL.

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Abstract

The invention discloses a menstruation stem cell preparation for treating pure skin injury and a preparation method thereof. The invention aims to solve the problems of dissatisfactory repairing and obvious cicatrization of the medicine in the prior art for repairing the tissues after the pure skin injury. The menstruation stem cell preparation comprises a cell disruption fluid and rhEGF and KGF added into the cell disruption fluid; the final concentration of rhEGF is 0.05-0.1ug / mL; the final concentration of KGF is 0.1-0.5ug / mL; the cell disruption fluid is prepared in the manner of crushing the menstruation stem cells in the concentration of 1*10<7>-2*10<9> pieces / mL and then performing filtration sterilization. The menstruation stem cell preparation has the advantage of better tissue repairing effect.

Description

technical field [0001] The invention relates to a menstrual blood stem cell preparation, in particular to a menstrual blood stem cell preparation for treating simple skin damage and a preparation method thereof. Background technique [0002] Simple skin injury means that the skin injury only accumulates the whole layer of skin and the superficial part of subcutaneous tissue. Severe skin damage will cause deep tissue necrosis. If it is not handled properly, severe ulceration will occur, and it will not heal for a long time. Skin wound healing refers to the repair process after the skin tissue is severed or damaged due to external force, including the regeneration of various tissues and the complex combination of granulation tissue hyperplasia and scar tissue formation, showing the synergistic effect of various processes. [0003] Regeneration of the epidermis and other tissues occurs within 24 hours of trauma, and the basal cells at the edge of the wound begin to proliferate...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/18A61K35/48A61P17/00
CPCA61K35/48A61K38/18A61K38/1808A61K2300/00
Inventor 杨远徐子林于洪涛李巍袁祥李昕怡吴启晓
Owner 成都远山博桥生物科技有限公司
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