Kit for detecting alpha and beta globin gene sequences in same tube

Abstract

The invention discloses a kit for detecting alpha and beta globin gene sequences in the same tube. The kit comprises an amplification primer and probes for identifying deletion of the loci including -SEA, -alpha <3.7> and -alpha <4.2> of the alpha globin gene sequence, the mutation of the loci including CD30, CD31, CD59, WM, QS and CS of the alpha globin gene sequence, and the mutation of the loci including -82, -80, -79, -78, Cap+1, Cap40-43, Int, CDs14 / 15, CD17, CD19, CD26, CDs27 / 28, CD30, IVSI-1, CD31, CD37, CDs41 / 42, CD43, CDs71 / 72, CD95, IVSII-5 and IVSII-654 of the beta globin gene sequence.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a kit for detecting alpha and beta globin gene sequences in one tube. Background technique [0002] Thalassemia (thalassemia, referred to as thalassemia) is a kind of monogenic genetic hemolytic disease caused by the imbalance of globin chain synthesis. This disease was first discovered in the peoples along the Mediterranean Sea, and it is one of the most common blood genetic diseases that have the greatest impact on human health in the world. The globin in normal adult hemoglobin is composed of 4 peptide chains, namely α, β, γ, and δ chains. Each chain has a corresponding gene code, and point mutations, deletions or insertions of these genes will cause the peptides to varying degrees. Chain synthesis disorders, resulting in changes in hemoglobin components, resulting in anemia. [0003] Thalassemias are usually divided into four types: α, β, δβ, and δ, of which α and β thalassemia...

AI Technical Summary

Problems solved by technology

This method has simple equipment, but the detection time is long, the detection sensitivity is low, and the operation is cumbersome, so it is not suitable for the detection of large sample volume.

[0008] Although the fluorescent PCR melting curve method is easy to operate, it is limited by the instrument itself and is not suitable for the test of direct expansion samples, nor is it suitable for the detection of multiple sites in the same sample or the detection of large sample volumes.

In contrast, the microarray chip method has a high detection throughput, but the required equipment is expensive and difficult to promote

Nucleic acid sequencing is the gold standard, and it is also the method that obtains the most information in one reaction, but its operation is complicated, the detection time is long, and the equipment and reagents are expensive. It is not conducive to popularization at this stage.

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