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Low-chloropropanol microbial oil and method for preparing same

A microbial oil, chloropropanol technology, applied in the directions of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problem of high content of glycidyl ester or chloropropanol, reduce the content of chloropropanol, and reduce the applicability of chloropropanol. Good results

Active Publication Date: 2017-12-01
CABIO BIOTECH WUHAN CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention provides a low-chloropropanol microbial oil and its preparation method to overcome the above problems or at least partially solve the above problems, so as to solve the technical problem of high glycidyl ester or chloropropanol content in edible microbial oils

Method used

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  • Low-chloropropanol microbial oil and method for preparing same
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  • Low-chloropropanol microbial oil and method for preparing same

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Experimental program
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Effect test

Embodiment 1

[0047] Alpina alpina is used as the fermentation strain, and when the oil content in the fermented liquid obtained from fermentation is 5.31%, luteolin with a mass of 0.025% of the fermented liquid is added, and the fermented liquid is filtered through a plate frame to obtain concentrated thallus, and the concentrated thallus is baked. After drying, add hexane and raise the temperature to 80° C., shear and stir, and obtain arachidonic acid (ARA) crude oil after screen filtration and precipitation. The crude oil is degummed by acid method, refined by alkali, decolorized and deodorized to produce microbial oil. The microbial oil is further subjected to molecular distillation under the conditions of 0.2 Pa and 185° C. to obtain refined microbial oil. The main components in the resulting product are shown in Table 1.

[0048] Table 1 Comparison of main indicators of microbial oil

[0049]

Embodiment 2

[0051] Alpina alpina is used as the fermentation strain, and when the oil content in the fermented liquid obtained from fermentation is 4.53%, 0.01% oleanolic acid in the mass of the fermented liquid is added, and the fermented liquid is filtered through a plate frame to obtain concentrated thallus, concentrated thallus After drying, add hexane and raise the temperature to 80° C., shear and stir, and filter through a sieve to obtain arachidonic acid (ARA) crude oil. The crude oil is subjected to acid degumming, alkali refining, decolorization, and deodorization to produce microbial oil, and the microbial oil is further subjected to molecular distillation at 0.5 Pa and 195°C to obtain refined microbial oil. The main components in the obtained microbial oil are shown in Table 2.

[0052] Table 2 Comparison of main indicators of microbial oil

[0053]

Embodiment 3

[0055] Alpina alpina is used as the fermentation strain, and when the oil content in the fermented liquid obtained from fermentation is 6.27%, 0.01% oleanolic acid in the mass of the fermented liquid is added, and the fermented liquid is filtered through a plate and frame to obtain concentrated thallus, concentrated thallus After drying, add hexane and raise the temperature to 80° C., shear and stir, and filter through a sieve to obtain arachidonic acid (ARA) crude oil. The crude oil is subjected to acid degumming, alkali refining, decolorization, and deodorization to produce microbial oil, and the microbial oil is further subjected to molecular distillation at 0.4Pa and 150°C to obtain refined microbial oil. The main components in the obtained microbial oil are shown in Table 3.

[0056] Table 3 Comparison of main indicators of microbial oil

[0057]

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Abstract

The invention provides low-chloropropanol microbial oil and a method for preparing the same. Lipase inhibitors are added into fermentation broth fermented from microbial strains, and extraction, refining and deodorization treatment is carried out, so that the contents of glycidyl ester and / or chloropropanol in the obtained microbial oil can be reduced; the activity of lipases in microbial cells in the fermentation broth can be inhibited by the lipase inhibitors. Compared with the prior art, the low-chloropropanol microbial oil and the method have the advantages that the technical problems of required additional corresponding treatment procedures when the content of chloropropanol is about to be reduced and the high content of chloropropanol in final products can be solved; the microbial oil is low in chloropropanol content, treatment technologies are simple, and existing technological processes do not need to be changed.

Description

technical field [0001] The invention relates to the preparation technology of microbial oil, more specifically, relates to a low-chloropropanol microbial oil and a preparation method thereof. Background technique [0002] Chloropropanol is a general term for a class of compounds formed after the hydroxyl group in glycerol is replaced by chlorine. According to the number and position of chlorine atoms substituted, chloropropanol can be divided into two types: monochloropropanediol and dichloropropanol. Among them, 3-chloro-1,2-propanediol (3-MCPD) has the effect of interfering with the balance of sex hormones in the human body, reducing the number of sperm and weakening the reproductive capacity of male animals. In addition, it also has chronic toxicity, carcinogenicity and neurotoxicity. [0003] Microbial oil is prepared by using microorganisms such as yeast, mold, bacteria or algae under certain conditions, using carbohydrates, hydrocarbons or ordinary oil as carbon sourc...

Claims

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Application Information

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IPC IPC(8): C12P7/64C12R1/645C12R1/89
CPCC12P7/6463
Inventor 李翔宇陆姝欢陈龙周强马凡提汪志明
Owner CABIO BIOTECH WUHAN CO LTD
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