A kind of veterinary traditional Chinese medicine immunopotentiator and application thereof
An immune enhancer and traditional Chinese medicine technology, applied in veterinary vaccines, vaccines, antiviral agents, etc., can solve the problems of rising breeding costs, physiological metabolism restrictions, loss, etc., to restore the body's normal immunity, improve vaccine quality, improve effect of immunity
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Embodiment 1
[0015] Embodiment 1: A traditional Chinese medicine immunopotentiator for veterinary use is characterized in that it is prepared from the following raw materials in parts by weight: 10 grams of angelica polysaccharide, 6 grams of lentinan, 4 grams of aloe polysaccharide, hydrogenated polyoxyethylene 20 grams of castor oil, 10 grams of absolute ethanol, 30 grams of ethyl acetate, and 20 grams of water for injection.
[0016] Under sterile conditions, first mix 20 grams of polyoxyethylene hydrogenated castor oil and 10 grams of absolute ethanol, then add 30 grams of ethyl acetate, mix well, and obtain a mixed solution 1, take 10 grams of angelica polysaccharide, 6 grams of mushroom Polysaccharide, 4 grams of aloe polysaccharide and 20 grams of water for injection were mixed evenly to obtain a mixed solution 2, and the mixed solution 2 was added to the mixed solution 1, and the stirring was continued during the addition process, and the stirring was continued for 15 minutes until ...
Embodiment 2
[0017] Example 2: Screening of Angelica polysaccharide, Lentinan, and Aloe Polysaccharide Optimal Ratio
[0018] (1) Grouping and disposal
[0019] 3-factor 3-level orthogonal design L9 (3 4 ) grouping, each polysaccharide was taken as a factor, and different concentrations were taken as level items. The results of the orthogonal design grouping are shown in Table 1. Kunming mice weighing 18-22 g were randomly divided into 11 groups, 10 mice in each group, and labeled with picric acid. Among them, groups 1-9 are polysaccharide groups with orthogonal design: the corresponding polysaccharide prescriptions of traditional Chinese medicine in each group are administered by intragastric administration; normal control group and cyclophosphamide control group: normal saline is administered intragastrically. The intragastric volume of mice in each group was 0.2 ml, and the drug was administered once a day for 14 consecutive days. Except for the normal control group, mice in other gr...
Embodiment 3
[0051] Example 3: Antibody effect of traditional Chinese medicine enhancer on chicken Newcastle disease vaccine (La Sota strain)
[0052] 50 7-day-old AA white-feathered broilers were randomly and equally divided into ① normal saline group, ② Newcastle disease inactivated antigen group, ③ traditional Chinese medicine enhancer group, ④ traditional Chinese medicine enhancer + inactivated antigen group, ⑤ commercially available Newcastle disease inactivated vaccine ( La Sota strain) group was immunized twice at the age of 7 days and 14 days by chest intramuscular injection, respectively, at 14, 21, 28, and 35 days after immunization, the blood was collected to separate the serum, and the content of the corresponding Newcastle disease antibody was detected by the HI method. The test results are shown in Table 3.
[0053] Table 3 Antibody detection results of chicken Newcastle disease vaccine (La Sota strain)
[0054] group 14 days 21 days 28 days 35 days sa...
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