Preparation method and application of high-activity anti-aging component with natural source

A natural source, anti-aging technology, applied in the field of ginsenosides, can solve the problems of high production cost, cumbersome process, and complicated products, and achieve the effect of low production cost and few process steps

Inactive Publication Date: 2017-11-07
肖永坤
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Using mineral acid to heat in a microwave reactor, hydrolyze the total saponins of Panax notoginseng to prepare Rg3 and Rh2, but the author did not explain the configuration of the generated Rg3 and Rh2; Chen, Yingjie reported the hydrolysis of ginsenosides using alkali energy[Chen Yingjie ,Chemical&Pharmaceutical Bulletin 1987,35(4),1653-5], the hydrolysis product is 20(S)Rg2 to generate 20(S)Rh1 and aglycone, but the acid-base hydrolysis method cannot prepare saponin F1
It is difficult to make a breakthrough on this method, and related reports in recent years have mainly focused on the treatment after hydrolysis (invention patent, preparation method of ginsenoside Rh1, publication number CN 101671384A)
[0022] The acid-base hydrolysis method has obvious disadvantages: the reaction conditions are severe, the pollution is large, the energy consumption is high, and it does not meet the current environmental protection requirements; the product is complicated and difficult to separate and purify; Unknown ingredients, efficacy and safety are not clear; the reaction process is difficult to control, and the ingredients of different batches are different; it is harmful to operators and is not suitable for industrial production
[0030] In summary, the method of preparing rare saponins by biotransformation has outstanding advantages, but the methods reported so far have disadvantages such as high production cost, low conversion rate, large volume of fermentation broth, cumbersome process, complicated operation, and difficult separation and purification.
Existing reports about the preparation of F1 also fail to get rid of these shortcomings, and for the preparation of F1, 20-(R, S) Rg2, Rg4, Rg6, 20-(R, S) Rh1, Rk3, Rh4, aglycone mixture The method has not been reported yet

Method used

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preparation example Construction

[0065] A method for preparing natural-derived high-activity anti-aging ingredients is carried out according to the following steps:

[0066] 1) Screen a single aerobic strain that can produce triol ginsenoside hydrolase;

[0067] 2) Select the plant tissue of the genus Ginseng as the main raw material, and add at least one of wheat bran, wheatgrass, grain flour, and an appropriate amount of inducer; stir well and add an appropriate amount of water; the water content of the solid medium after stirring is uniform Between 30 and 70%;

[0068] 3) After mixing, quickly heat to above 100°C and keep it for 2-30 minutes for sterilization, and then quickly cool to below 45°C; after sterilization, insert the strains, which are the only bacteria, molds and yeasts One, the inoculation amount is 0.001% to 50% of the total weight of the culture medium; the culture medium is fully stirred by rotating the fermentor, and sterile air is introduced; warm water is introduced into the interlayer of the ...

Embodiment 1

[0075] First prepare fermented bacteria seeds: take 100g of ginseng stems and leaves and crush them to about 10 mesh, mix with 900g of wheat bran, add 1L of water, stir evenly, put into a triangular flask, sterilize at 120°C for 20 minutes, and then join the research team after cooling A selected Aspergillus oryzae (Dongming Wang, Process Biochemistry, 2012, 47(1), 133-138), cultured at 28°C for 7-8 days, is the fermented seed.

[0076] Large-scale fermentation in a fermenter: crush 30kg ginseng stems and leaves to about 10 meshes, add 50kg wheat bran, 5kg soybean meal, and 80kg water to mix evenly, put them into the fermenter, steam to 100℃ for sterilization, 12 minutes later, cool To 35°C, then insert the prepared seeds for fermentation culture. Filtered sterile air is always introduced during the cultivation process, the temperature of the fermenter interlayer is set to 30°C, the temperature is kept for 24h, and then the product temperature is always kept between 28-30°C, and ...

Embodiment 2

[0078] The fermented seeds were prepared according to the method in Example 1.

[0079] Take 20kg American ginseng fibrous root, crush it to 10-20 mesh, mix it with 70kg wheat bran and 10kg Sophora japonica, mix it with 50kg water, stir evenly, put it into the fermenter, heat it with steam to 100℃ for 10 minutes, and quickly cool it to 40℃. Then insert the seed. Sterile air is always introduced into the fermenter, and the temperature of the interlayer is set to 30℃. After keeping the temperature for 24 hours, keep the product temperature between 28~30℃. After 12 days of fermentation, the fermentation product is rich in F1, such as figure 2 Shown.

[0080] Then add 500L methanol to the fermentor, extract 3 times at 50℃, 3h each time, concentrate the extract under reduced pressure at 25~70℃, -0.01Mpa~1Mpa until there is no methanol residue, add 50L cyclohexane for degreasing twice, Put the degreased solution on a 30LAB-8 macroporous adsorption resin column, and repeatedly load the...

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Abstract

The invention discloses a preparation method of a high-activity anti-aging component with a natural source. The preparation method comprises the following steps of screening out a proper aerobic single strain; using the plant tissue of a ginseng plant as a main raw material, adding at least one of wheat bran, wheat straw, grain flour and a rice husk, water and other inductors, sufficiently agitating, and sterilizing; inoculating the strain, and fermenting, wherein a fermentation product is a ginseng product which is enriched in F1 and has high anti-aging activity; adding lower alcohol to extract a ginsenoside mixture; extracting a hydrolytic enzyme from an extracted filter residue by using water or a buffer solution; converting ginsenoside by utilizing the hydrolytic enzyme, so that the high-activity anti-aging component with the natural source can be obtained. By using the preparation method, the total ginsenoside does not need to be divided into diol saponin PPD (Protopanaxadiol) and triol saponin PPT (Protopanaxatriol); the ginsenoside glycosidase also does not need to be specially prepared; an anti-aging component F1 is directly prepared through a fermentation method.

Description

Technical field [0001] The invention relates to the technical field of ginsenosides, in particular to a preparation method and application of a natural-derived highly active anti-aging component. Background technique [0002] Wild ginseng plants are intermittently distributed in East Asia and North America, and more than ten species have been discovered. In my country, the ginseng plants grown are mainly ginseng from the three northeast provinces, panax notoginseng from Yunnan and Guangxi, and American ginseng from northeast and Shandong. A small amount of bamboo joint ginseng and pearl ginseng are also used for planting, but the planting area is very small. [0003] Ginsenoside is the most important active ingredient in ginseng plant. Saponins are glycosides connected by glycosidic bonds by glycosylated and non-glycosyl ligands. Saponins are divided into different types according to the difference between glycosylated and ligands and the position of glycosidic bonds. According ...

Claims

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Application Information

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IPC IPC(8): C12P33/20C12P33/00A61K8/63A61Q19/02A61Q19/08A61Q17/04A61K36/258A61P39/06A23L33/105
CPCA23L33/105A23V2002/00A61K8/63A61K36/258A61Q17/04A61Q19/02A61Q19/08C12P33/00C12P33/20A23V2200/302A23V2250/2124
Inventor 肖永坤王东明栾桂花
Owner 肖永坤
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