Method for extracting, separating and purifying four main anthocyanins from blackcurrant residue

Abstract

The invention relates to a method for extracting, separating and purifying four main anthocyanins from the blackcurrant residue. The object is to provide a simple, economic, green, eco-friendly, and high-yield method for extracting, separating and purifying four main high-purity anthocyanins from the blackcurrant residue. According to the method, the blackcurrant waste residue produced from industrial production is taken as a raw material, and homogenized pulverized coupling enzyme hydrolysis technology, negative-pressure cavitation reinforced extraction technology, macroporous adsorption resin concentration technology, medium-pressure quick-flash reverse chromatographic separation technology and low-temperature crystallization and recrystallization technology are adopted to obtain four anthocyanin monomer ingredients including delphinidin 3-O-glucoside, delphinidin 3-O-rutinoside, cyanidin 3-O-glucoside, and cyanidin 3-O-rutinoside and having the purity reach 95%. The method is abundant and available in raw materials, short in time consumption, less in solvent consumption, high in purity of obtained anthocyanins, low in production cost, and suitable for massive industrial production.

Description

technical field [0001] The invention relates to a method for extracting, separating and purifying four high-purity anthocyanins from blackcurrant pomace, and belongs to the field of separation and purification of plant secondary metabolites. Background technique [0002] Black currant, the scientific name of black currant (Ribes nigrruu L.), commonly known as black bean fruit, alias black summer fruit, is a small deciduous shrub of the saxifrage family (saxifragaceae) and the genus Ribes, mainly produced in my country In Northeast China and northern Xinjiang, the fruit is small dark purple-brown berries, which can be eaten or processed into fruit juice, jam and other foods. Black currant is rich in sugar, organic acids, multivitamins, anthocyanins, active minerals and special aromatic components, and has high nutritional and medicinal value. Wherein the total anthocyanin content is 3.5mg / g fresh fruit weight (Zhang Zhidong 2002). The currently known curative functions of bl...

AI Technical Summary

Problems solved by technology

So far, the methods for separating and purifying anthocyanin monomers generally use semi-preparative high-performance liquid chromatography or high-speed countercurrent chromatography. These methods generally have shortcomings such as expensive equipment and cumbersome methods, and cannot carry out large-scale separation and purification. It is difficult to be applied in actual production, which makes anthocyanin products lack in large quantities, and the price is very expensive

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