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Method for simultaneously determining four components in herba cynomorii

A technology of Cynomorium cynomorium and chemical composition, which is applied in the field of quantitative analysis of the quality of Chinese medicinal materials, can solve the problems of large difference in solubility and no quantitative analysis, etc., and achieve the effect of simple method, short time consumption and good repeatability

Inactive Publication Date: 2017-10-24
CHINESE RES ACAD OF ENVIRONMENTAL SCI +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The 2015 edition of the Pharmacopoeia only stipulates the qualitative identification of Cynomorium cynomorium, but there is no quantitative analysis method
However, how to realize the simultaneous determination of various flavonoids with large differences in solubility has not been investigated by scholars

Method used

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  • Method for simultaneously determining four components in herba cynomorii
  • Method for simultaneously determining four components in herba cynomorii
  • Method for simultaneously determining four components in herba cynomorii

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0040] Preparation of the test solution: Take about 2 g of crushed Cynomorium medicinal material powder (place of origin Gansu) (passed through a No. 4 sieve), accurately weighed, place in a 50 mL centrifuge tube, add 10 mL of pure water, and sonicate for 30 min ( Control the temperature at about 55°C), shake well, centrifuge at 10,000rpm for 10min, pipette the supernatant into a 25mL brown volumetric flask, add 8mL and 5mL of water to the residue in turn, shake on a vortex oscillator for 2min, and treat twice as above, Combine the supernatants from three centrifugations into the same 25mL brown volumetric flask, add water to make up to the mark, shake well, filter, and take the filtrate to obtain the final product.

[0041] Preparation of reference substance solution; accurately weigh gallic acid, protocatechuic acid, catechin, and phlorizin in an appropriate amount in the same volumetric flask, add an appropriate amount of 50% methanol to dissolve, and dilute with ultrapure w...

Embodiment 1

[0042] Embodiment 1 Cynomorium medicinal material content determination

[0043] Blank test: Considering the treatment process of the test product in this experiment, it is sufficient to use ultrapure water as a blank sample. The blank sample chromatogram is attached figure 1 shown, compare figure 1 , figure 2 Visible, in measuring range, blank sample does not influence the mensuration of need testing product, Cynomorium cynomorium need testing product solution HPLC collection of illustrative plates is as follows: image 3 shown.

[0044] Preparation of standard curve: Accurately weigh 10.97 mg of gallic acid (110-831-201605, 90.8%, China Institute for Food and Drug Control), 4.75 mg of protocatechuic acid (110.809-200604, China Institute for Food and Drug Control), pediatric Tea element (877-200001, China Institute for Food and Drug Control) 10.52mg, phlorizin (Y-029-150705, 98.96%, Chengdu Ruifensi Biotechnology Co., Ltd.) 7.16mg in a 25mL brown volumetric flask, add A...

Embodiment 2

[0087] The mensuration of multiple components in the Chinese patent medicine of embodiment 2

[0088] This embodiment is basically the same as Example 1, the difference is that the Chinese patent medicine Shenkangning Tablets (batch number: 1601002) is used to replace Cynomorium cynomorium medicinal material, the preparation method of the test solution is slightly changed, and the chromatogram of the test solution of Shenkangning is as follows Figure 8 shown. (Pictures are not results, measurement results are data)

[0089] Preparation of the test solution: take 20 capsules of Shenkangning tablets, a Chinese patent medicine containing Cynomorium, remove the coating from the sugar-coated tablets, accurately weigh, grind, take about 2g, after accurately weighing, place in a 50mL centrifuge tube, Add 10mL of pure water, sonicate for 30min (the temperature is controlled at about 55°C), shake well, centrifuge at 10000rpm for 10min, transfer the supernatant to a 25mL brown volumet...

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Abstract

The invention provides a method for simultaneously determining four components in a herba cynomorii-containing sample, in particular to a method for simultaneously determining gallic acid, protocatechuic acid, catechin and phlorizin in the herba cynomorii-containing sample by using a high performance liquid chromatography (HPLC). According to the method, a standard product solution and a sample solution are determined by using the HPLC, and multi-gradient elution is carried out by using acetonitrile and 0.05% of a glacial acetic acid solution as mobile phases, so that the four components can be determined. Compared with the chemical composition determination method in the prior art, the method provided by the invention is simple in operation process, good in repeatability and high in accuracy, avoids the consumption of time and cost, can be used as an effective method for quantitatively controlling the quality of herba cynomorii, and is also used for detecting herba cynomorii-related effective components in Chinese patent medicines.

Description

field of invention [0001] The invention belongs to the field of traditional Chinese medicines, and in particular relates to a quantitative analysis technology for the quality of Chinese medicinal materials. Background technique [0002] Cynomorium is commonly known as "the elixir", also known as rusty iron stick, ground hair ball, and sheep lock. It is the dry fleshy stem of Cynomorium songaricum Rupr. [1] . Distributed in Gansu, Xinjiang, Inner Mongolia, Qinghai and other places, produced in the sandy land of Hexi Corridor, Tengger Desert, west of Hunshandake Sandy Land [2] . It has the functions of invigorating kidney yang, nourishing essence and blood, moistening intestines and laxative. It is clinically used for deficiency of kidney yang, deficiency of essence and blood, flaccidity of waist and knees, impotence and slippery essence, intestinal dryness and constipation [3] . The research work of modern phytochemists shows that Cynomorium contains many types of active ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 关潇任梦云李俊生魏静娜刘征辉赵志平肖能文罗遵兰
Owner CHINESE RES ACAD OF ENVIRONMENTAL SCI
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