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Enzymatic production technology of chitosan oligosaccharide

A production process and technology of chitosan oligosaccharide, applied in the field of chitosan oligosaccharide enzymatic production process, can solve the problems of high cost, poor chitosan degradation effect, unfavorable large-scale production and the like, and achieve improved quality, uniform size, Yield-enhancing effect

Inactive Publication Date: 2017-09-19
赵景卫
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, enzymatic hydrolysis is more commonly used. For example, chitinase, chitosanase and lysozyme can be used for specific enzymatic degradation. However, specific enzymatic degradation is limited by high cost, which is not conducive to large-scale production.
Non-specific enzymatic degradation can solve the problem of high cost of specific enzymatic methods, but the existing non-specific enzymatic degradation, such as the use of commercial lipase, protease, pectinase, etc., has a poor degradation effect on chitosan , to be further improved

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] a Add chitosan to acetic acid solution to prepare chitosan solution, the mass percent concentration of chitosan solution is 15%; the mass percent concentration of acetic acid solution is 1%.

[0022] B adds hydrogen peroxide solution in the chitosan solution that step a makes, the mass percentage concentration of hydrogen peroxide solution is 40%, and the mass ratio of chitosan and hydrogen peroxide solution is 1: 0.5; Stir evenly, carry out For the degradation reaction, control the degradation temperature to 80° C., and the degradation time to 4 hours. After the degradation is completed, a degradation liquid is obtained.

[0023] c Cool the degradation solution obtained in step b to room temperature, then add saturated sodium bicarbonate solution to adjust the pH value to 6.2, then add β-glucosidase for the first step of enzymolysis, the amount of β-glucosidase added is 1% of the mass of the polysaccharide; the temperature of the enzymolysis reaction is controlled at 3...

Embodiment 2

[0029] a Add chitosan to acetic acid solution to prepare chitosan solution, the mass percent concentration of chitosan solution is 20%; the mass percent concentration of acetic acid solution is 2%.

[0030] B adds hydrogen peroxide solution in the chitosan solution that step a makes, the mass percent concentration of hydrogen peroxide solution is 35%, and the mass ratio of chitosan and hydrogen peroxide solution is 1: 0.3; Stir evenly, carry out For the degradation reaction, the degradation temperature is controlled to be 60° C., the degradation time is 4 hours, and the degradation solution is obtained after the degradation is completed.

[0031] c Cool the degradation solution obtained in step b to room temperature, then add saturated sodium bicarbonate solution to adjust the pH value to 6.5, then add β-glucosidase for the first step of enzymolysis, the amount of β-glucosidase added is 1.2% of the mass of the polysaccharide; the temperature of the enzymolysis reaction is cont...

Embodiment 3

[0037] a Add chitosan to acetic acid solution to prepare chitosan solution, the mass percent concentration of chitosan solution is 18%; the mass percent concentration of acetic acid solution is 1.5%.

[0038] B adds hydrogen peroxide solution in the chitosan solution that step a makes, and the mass percent concentration of hydrogen peroxide solution is 40%, and the mass ratio of chitosan and hydrogen peroxide solution is 1: 0.3; Stir evenly, carry out For the degradation reaction, control the degradation temperature to 70° C., and the degradation time to 4 hours. After the degradation is completed, a degradation liquid is obtained.

[0039] c Cool the degradation liquid obtained in step b to room temperature, then add saturated sodium bicarbonate solution to adjust the pH value to 6.3, then add β-glucosidase for the first step of enzymolysis, the amount of β-glucosidase added is 1.2% of the mass of the polysaccharide; the temperature of the enzymolysis reaction is controlled a...

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Abstract

The invention discloses an enzymatic production technology of chitosan oligosaccharide. The technology comprises steps as follows: a, chitosan is added to an acetate solution, and a chitosan solution is prepared; b, a hydrogen peroxide solution is added to the chitosan solution and stirred uniformly, a mixed solution is subjected to a degradation reaction, and a degraded solution is obtained after degradation; c, the degraded solution is cooled to the room temperature, pH value is regulated to 6.2-6.5, then beta-glucosidase is added for primary enzymolysis, and a primary enzymatic hydrolysate is obtained; d, alpha-1, 4-glucan glucohydrolace is added to the primary enzymatic hydrolysate for secondary enzymolysis, and a secondary enzymatic hydrolysate is obtained; e, pH value of the secondary enzymatic hydrolysate is regulated to 6-6.5, the secondary enzymatic hydrolysate is subjected to rough filtration, ultrafiltration, nanofiltration, spray drying and sterilization sequentially, and a finished chitosan oligosaccharide product is obtained. According to the enzymatic production technology of chitosan oligosaccharide, the yield of chitosan oligosaccharide is greatly increased, molecular weight of the finally prepared chitosan oligosaccharide product can be smaller than 1500, the size is relatively uniform, and besides, the product contains no monosaccharide, therefore, the quality of the chitosan oligosaccharide product is effectively improved.

Description

technical field [0001] The invention relates to a production process of chitosan oligosaccharides, in particular to an enzymatic production process of chitosan oligosaccharides. Background technique [0002] Chitosan oligosaccharide is also called chitosan oligosaccharide and oligochitosan. It is an oligosaccharide product with a polymerization degree between 2 and 20 obtained by degrading chitosan. Its molecular weight is ≤3200Da. A low molecular weight product with great functional effects and high biological activity. It has many unique functions such as high solubility that chitosan does not have, fully soluble in water, easy to be absorbed and utilized by organisms, and its effect is 14 times that of chitosan. Oligochitosan is the only positively charged cationic basic amino oligosaccharide in nature, and it is animal cellulose. [0003] Studies have proved that chitosan oligosaccharides can improve immunity, inhibit the growth of cancer cells, promote the formation o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/26C12P19/14C08B37/08
CPCC12P19/26C08B37/003C12P19/14
Inventor 赵景卫
Owner 赵景卫
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